Peptide Quantification Kits

MtoZ Biolabs Peptide Quantification Kits: Accurate Quantification and Consistent Sample Loading - A Critical Step for Standardizing Peptide Concentrations Prior to Mass Spectrometry

In mass spectrometry-based proteomics workflows, achieving accurate and consistent peptide loading is essential for reliable quantitative comparisons across samples. The MtoZ Biolabs Peptide Quantification Kits provide a sensitive, rapid, and user-friendly fluorescence microplate assay specifically designed for precise peptide quantification following proteolytic digestion and desalting purification prior to MS injection. Utilizing a unique amine-reactive fluorescent labeling strategy, the kits enable accurate determination of peptide concentrations for both synthetic peptides and complex peptide mixtures. This facilitates standardized loading among different samples, minimizes technical variability arising from loading deviations, and ultimately enhances the comparability of downstream mass spectrometry data and the reliability of resulting biological interpretations.

Advantages

1. High Sensitivity with a Broad Linear Range

MtoZ Biolabs Peptide Quantification Kits achieve a detection limit of 5 μg/mL and a linear range of 5–1000 μg/mL, enabling accurate quantification across sample types ranging from scarce and valuable materials to routine preparations.

2. Fast and Stable Performance with Minimal Handling

Operating in a “mix-and-read” format, the fluorescent labeling reaction proceeds rapidly, and the resulting signal stabilizes within 5 minutes and remains sustained for several hours, markedly reducing sample preparation time.

3. Excellent Reproducibility and Quantitation Accuracy

The kit includes high-quality peptide digestion standards for generating reliable calibration curves, serving as internal references that substantially enhance inter-batch reproducibility and quantitative accuracy.

4. Broad Compatibility with MS Sample Preparation Buffers

The detection system tolerates commonly used components such as acetonitrile and formic acid, allowing direct quantification of desalted peptide solutions without any additional processing steps.

5. Minimal Sample Consumption

Only 10 μL of sample is required for analysis, preserving limited and valuable samples.

6. A Standardized Component of MS “Equal Loading” Workflows

The kit constitutes a key step toward standardizing peptide loading prior to mass spectrometry, effectively minimizing data variability caused by inconsistent sample loading and providing a reliable analytical basis for differential proteomics, biomarker discovery, and related research applications.

Technical Support

1. Protocol Downloads

MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.

2. Customized Service Support

Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.

3. FAQ and Expert Assistance

We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.