Iodoacetamide (IAM) is a thiol-alkylating reagent commonly used in pre-digestion processing for proteomics. It selectively blocks cysteine thiol groups, stabilizes the reduced state by preventing disulfide bond reformation, thereby improving digestion consistency and data reproducibility, and is widely applied in proteomics sample preparation and LC-MS/MS analysis.
Product Overview
In proteomics sample preparation, insufficient pre-digestion treatment often becomes a key limiting factor affecting data quality, mainly reflected in the following aspects:
1. Disulfide Bond-Restricted Exposure of Proteolytic Sites
Disulfide bonds in native proteins stabilize higher-order structures, limiting access of proteases such as trypsin.
2. Re-Pairing of Thiols After Reduction
Cysteine residues may reform disulfide bonds during digestion, leading to conformational heterogeneity and inconsistent results.
3. Insufficient Digestion Reproducibility and Quantitative Stability
Unstable thiol states amplify sample-to-sample variation, reducing quantitative accuracy and batch comparability.
4. Increased Complexity of Downstream MS Data
Nonspecific reactions and structural heterogeneity complicate database searching and data interpretation.
Advantages
✅ High-Specificity Thiol Alkylation
Selectively blocks cysteine thiols, prevents disulfide bond reformation, and stabilizes protein structural states.
✅ Neutral Modification with High MS Compatibility
Introduces no additional charge, facilitating peptide ionization and quantitative analysis.
✅ Significantly Improved Digestion Consistency
Enables more uniform and reproducible digestion, increasing protein sequence coverage.
✅ Broad Compatibility with Mainstream Proteomics Workflows
Highly compatible with label-free, TMT, iTRAQ, and DIA/DDA strategies.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
