Kidney Disease Exosome Research Service
Kidney diseases are characterized by complex pathogenesis, subtle early symptoms, and narrow clinical intervention windows, requiring more sensitive, non-invasive diagnostic tools and precise disease classification strategies. Leveraging advanced multi-omics platforms and exosome research capabilities, MtoZ Biolabs introduces Kidney Disease Exosome Research Service offering a comprehensive research solution that focuses on the dual roles of exosomes in kidney disease development and progression. Our service provides end-to-end solutions, including exosome isolation and purification, functional exploration, omics interpretation, and biomarker screening.
Exosomes are widely present in urine, plasma, and cell culture supernatants. As essential mediators of intercellular communication, the proteins, lipids, miRNAs, and other active molecules they carry can regulate tissue homeostasis, inflammation, apoptosis, and other vital biological processes. In diseases such as acute kidney injury (AKI), chronic kidney disease (CKD), diabetic nephropathy (DN), lupus nephritis (LN), glomerulosclerosis, and kidney fibrosis, urine-derived exosomes have been shown to play a significant role in disease mechanism research, diagnostic biomarker discovery, and intervention target screening. They also exhibit potential regulatory effects in kidney stone formation and tissue repair.
Jin, C. et al. STEM CELLS INT. 2021.
Figure 1. Exosome-Mediated Therapeutic Pathways in Kidney Disease Models
Services at MtoZ Biolabs
MtoZ Biolabs focuses on both fundamental and translational kidney disease research needs, offering a customizable, modular exosome research service platform. Our services include, but are not limited to, the following key components:
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Comprehensive Exosome Analysis Services by MtoZ Biolabs |
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Ultracentrifugation and Microfiltration based Exosome Purification |
Classical method for enriching exosomes from large-volume samples; suitable for preparative applications. |
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Size Exclusion Chromatography (SEC) based Exosome Purification |
Gentle, size-based separation preserving exosome integrity; ideal for downstream functional studies. |
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High-purity isolation targeting specific exosome surface markers using antibody-conjugated platforms. |
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Physical Characterization |
Measures particle size distribution and concentration; widely used for routine exosome quality assessment. |
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Provides direct visualization of exosome morphology; considered the gold standard for structural validation. |
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Enables single-particle analysis of size, concentration, and zeta potential with high precision. |
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Confirms exosome identity and purity by detecting classical (e.g., CD63) and negative (e.g., Calnexin) markers. |
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Quantifies surface proteins and distinguishes subpopulations using fluorescent antibody labeling. |
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High-sensitivity single-particle analysis of exosome size, concentration, and surface marker expression. |
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Mass spectrometry-based profiling of exosomal proteins to reveal functional content and biomarkers. |
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Identifies low-molecular-weight metabolites in exosomes to explore their metabolic roles and signatures. |
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Comprehensive analysis of lipid species involved in membrane structure and signaling. |
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High-throughput sequencing of total RNA to reveal lncRNA, miRNA, and mRNA content. |
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Fluorescent or functional labeling for exosome tracking and uptake studies. |
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Custom engineering of exosomes for targeted delivery to disease-specific tissues or cells. |
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Incorporates therapeutic cargos such as RNA, proteins, or small molecules into exosomes. |
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Exosome-Associated Adeno-Associated Virus (AAV) Vector Production |
Combines exosomes with AAV vectors to enhance gene delivery and targeting efficiency. |
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Assesses cellular-level effects such as proliferation, migration, and immune modulation. |
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Evaluates biodistribution and biological activity of exosomes in animal models. |
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These services represent only a subset of our offerings. For customized analysis plans, please contact our technical team for expert consultation.
Analysis Workflow
Why Choose MtoZ Biolabs?
MtoZ Biolabs offers high-precision and reliable research support for kidney disease exosome studies, leveraging the following core strengths:
✅ Non-invasive sample sources suitable for clinical translation: We support urine and serum samples, avoiding the invasive risks of tissue biopsy.
✅ Cross-platform multi-omics integration: Our Kidney Disease Exosome Research Service integrates mass spectrometry, NGS, and high-throughput imaging, enabling collaborative analysis of proteins, nucleic acids, and metabolites.
✅ Flexible, customized service workflow: We offer both standardized processes and personalized designs tailored to diverse research objectives.
✅ Comprehensive data delivery system: We provide raw data, differential analysis, pathway enrichment, functional annotation, and visualized reports for easy interpretation.
Sample Submission Suggestions
Note: Detailed SOPs for sample collection, storage, and shipment are available upon request. Please contact us to receive your customized sample handling kit.
Applications
The Kidney Disease Exosome Research Service from MtoZ Biolabs is broadly applicable in basic research, translational medicine, and early diagnostic biomarker development. Core applications include:
· Exosome biomarker screening and validation: Identifying potential biomarkers for AKI, DN, CKD, LN, and other kidney diseases using exosomal components from urine and plasma to improve early diagnostic accuracy.
· Pathological mechanism exploration: Analyzing exosomes released from renal tubular cells, podocytes, and immune cells involved in immune activation, inflammatory response, and apoptosis.
· Tissue repair and therapeutic target discovery: Exploring exosome roles in kidney damage regeneration, such as inducing angiogenesis and inhibiting apoptosis, promoting regenerative therapy research.
· Non-invasive prognosis monitoring and efficacy assessment: Tracking changes in exosome composition to monitor disease progression and therapeutic responses, optimizing individualized interventions.
FAQ
Q1: What advantages do exosomes offer over traditional biomarkers in kidney disease research?
Exosomes, as naturally occurring nanoscale vesicles derived from cells, carry specific proteins, RNA, and lipids from their origin cells. They reflect the true pathological state of tissues and can be easily obtained from non-invasive body fluids like urine. In kidney disease research, their dynamic changes offer higher sensitivity and specificity, addressing the limitations of traditional markers like creatinine or urine protein in early diagnosis and classification. MtoZ Biolabs can assist researchers in exploring and validating novel kidney disease biomarkers based on exosomes.
What Could be Included in the Report?
1. Comprehensive experimental details (materials, instruments, and methods)
2. Raw data
3. Processed omics data files
4. Quality control reports (NTA, Western blot, TEM images)
5. Functional assay results
6. Bioinformatics analysis
7. Customized report based on project requirements
MtoZ Biolabs is committed to creating an integrated exosome research platform for kidney diseases, offering end-to-end services from exosome isolation and purification to omics analysis and functional validation. We help scientists explore the application value of exosomes in kidney disease research. We also provide exosome research services for other diseases, such as tissue regeneration, infectious disease, cardiovascular disease, and more. Please feel free to contact us for customized technical support solutions.
Related Services
Tissue Regeneration and Repair Exosome Research Service
Infectious Disease Exosome Research Service
Cardiovascular Disease Exosome Research Service
Cancer Exosome Research Service
How to order?
