Immunoaffinity Capture based Exosome Purification Service

Exosomes are extracellular vesicles (EVs) with diameters of approximately 30–150 nm, secreted by various cell types and enriched with proteins, nucleic acids, lipids, and other bioactive molecules. They are widely found in biological fluids such as serum, plasma, urine, and cerebrospinal fluid, serving as critical mediators of intercellular communication and participating in essential biological processes, including disease progression, signal transduction, and immune regulation.
Importantly, exosome membranes are rich in specific surface proteins (e.g., CD9, CD63, CD81) and receptor molecules, providing a solid foundation for the development of highly efficient immunoaffinity capture-based exosome isolation strategies.

 

Immunoaffinity Capture based Exosome Purification Service utilizes highly specific interactions—such as antibody-antigen and receptor-ligand binding—to selectively capture target exosomes from complex biological fluids or cell culture supernatants. This approach effectively eliminates non-specific contaminants commonly encountered in traditional isolation methods, achieving exosome isolation with high purity and specificity.


Leveraging advanced technology platforms and extensive expertise in exosome purification, MtoZ Biolabs offers efficient and customizable Immunoaffinity Capture based Exosome Purification Service to support exosome functional studies, biomarker discovery, and drug delivery research.

 

Technical Principles

The Immunoaffinity Capture based Exosome Purification Service takes advantage of abundant surface markers (such as CD9, CD63, CD81) and receptor molecules on exosome membranes. Antibodies or ligands immobilized on magnetic beads or solid-phase carriers specifically bind to exosomes, enabling precise isolation of target populations.

 

Through incubation, capture, and washing steps, non-exosomal particles and impurities are effectively removed. Exosomes are then gently eluted under mild conditions to preserve their integrity and biological activity, avoiding damage associated with ultracentrifugation. This approach is particularly well-suited for isolating exosomes from specific sources or subtypes for targeted research.

 



Yang, D. et al. Theranostics. 2020.

Figure 1. Schematic of Immunoaffinity-based Exosome Isolation

 

Analysis Workflow

To ensure optimal separation efficiency and functional integrity of exosomes, MtoZ Biolabs' Immunoaffinity Capture based Exosome Purification Service follows a standardized, high-precision workflow:

1. Sample Pretreatment: Remove cell debris and large-particle impurities to enhance sample purity.

2. Immunomagnetic Bead Incubation: Incubate samples with magnetic beads coated with target antibodies (or ligands) for specific exosome capture.

3. Washing: Eliminate unbound components to ensure high-purity exosome collection.

4. Gentle Elution: Release exosomes under mild conditions to maintain structural and functional integrity.

5. Quality Verification (Optional): Comprehensive characterization including particle size analysis, morphological observation, and marker protein detection.

 

Why Choose MtoZ Biolabs?

·  High Specificity: Enables targeted isolation of specific exosomes (e.g., tissue-specific or marker-positive), supporting precise research needs.

·  High Purity and Bioactivity: Effectively removes impurities and non-exosomal vesicles, yielding highly active exosomes suitable for sensitive functional studies.

·  Flexible Customization: Supports a broad range of antibodies (e.g., CD9, CD63, CD81, EpCAM, HER2) and ligands tailored to different disease models.

·  Broad Sample Compatibility: Applicable to various biological fluids and cell culture supernatants, including serum, urine, milk, and more.

·  Automated Platform: Integrated workstations minimize manual errors and improve efficiency and batch consistency.

·  One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

 

Applications

Our Immunoaffinity Capture based Exosome Purification Service is widely applicable in basic and translational research, drug development, and clinical diagnostics, including:

· Biomarker Screening and Validation: Identification and validation of exosome-associated biomarkers for cancer, neurodegenerative diseases, cardiovascular diseases, etc.

· Targeted Drug Delivery Development: Isolation of specific exosomes for use in drug delivery system design and functional validation.

· Exosome Functional Studies: Investigations into exosome roles in tumor metastasis, immune regulation, and intercellular communication.

· Microenvironment and Disease Mechanism Analysis: Isolation of microenvironment-associated exosomes to explore disease mechanisms, including tumor progression and inflammatory processes.

 

Case Study

Case 1: Immunoaffinity capture-based isolation of neuron-derived exosomes (NDEs)

In a recent study, immunoaffinity capture was successfully employed to isolate neuron-derived exosomes (NDEs), offering new insights into biomarker discovery for neurological diseases. Researchers used functionalized magnetic beads conjugated with CD63 and L1CAM antibodies to selectively isolate NDEs from serum. The size, morphology, and integrity of isolated exosomes were confirmed via dynamic light scattering (DLS) and transmission electron microscopy (TEM).
Furthermore, antibodies targeting AMPA receptors (GluR2/3) were utilized to isolate NDEs from the serum of stroke patients, and the expression of GluR2/3 proteins was verified through Western blot analysis. This study demonstrated that immunoaffinity capture is a powerful and precise method for isolating NDEs, supporting both biomarker discovery and mechanistic research in neurological diseases.

 



Yousif, G. et al. Neuromolecular Med. 2021.

 

MtoZ Biolabs' Immunoaffinity Capture based Exosome Purification Service based on this advanced technology, helps clients efficiently isolate specific exosome populations from complex biological samples, facilitating disease mechanism exploration and biomarker identification.

 

FAQ

Q1: Which exosomal markers are available for immunoaffinity capture?

Our Immunoaffinity Capture based Exosome Purification Service supports exosome separation based on common markers including CD9, CD63, CD81. We also offer customized protocols for specific markers such as EpCAM, HER2, tailored to various disease models.

 

Deliverables

1. High-purity exosome samples (cryopreserved, suitable for long-term storage and downstream applications)

2. Exosome particle size and concentration analysis report (e.g., NTA data)

3. Exosome marker protein detection report (e.g., CD9, CD63, CD81 expression analysis)

4. Comprehensive project summary report (including detailed workflows, operational parameters, and analytical data)

5. Optional downstream analysis services, such as exosomal RNA/proteomics profiling and functional assays

 

MtoZ Biolabs is dedicated to delivering efficient and high-quality Immunoaffinity Capture based Exosome Purification Services to clients worldwide. In addition to immunoaffinity capture, we offer multiple exosome isolation solutions, including Size Exclusion Chromatography (SEC), ultracentrifugation, and microfiltration, allowing for flexible and customized purification strategies based on diverse sample types and research objectives. Our comprehensive services support a full range of needs, from basic research to translational applications.


Contact us today to discuss your project and receive tailored solutions.