Ultracentrifugation and Microfiltration based Exosome Purification Service

Ultracentrifugation and Microfiltration based Exosome Purification Service is a specialized solution that integrates ultracentrifugation and microfiltration techniques to efficiently isolate and purify exosomes from plasma and other body fluids. As nanoscale vesicles (30–150 nm), exosomes have been widely acknowledged as promising biomarkers with significant clinical value in early disease diagnosis, personalized medicine, and biomarker discovery. Nevertheless, the complexity of protein backgrounds and heterogeneity of extracellular vesicles in body fluids make exosome purification a technical challenge in biomedical research.

 

To address this, MtoZ Biolabs provides a robust Ultracentrifugation and Microfiltration based Exosome Purification Service that significantly reduces non-exosomal contaminants and improves exosome purity and integrity, ensuring high-quality samples for downstream research.

 

Technical Principles

Ultracentrifugation and Microfiltration based Exosome Purification Service relies on the synergistic application of these two core techniques to maximize exosome purity and yield, ensuring optimal performance in a variety of biological fluid samples.

1. Ultracentrifugation

Ultracentrifugation separates exosomes from other components in biological fluids based on their differential sedimentation rates, primarily influenced by particle size and density. Regarded as the "gold standard" for exosome isolation, ultracentrifugation is often combined with sucrose density gradients to achieve higher purity. The standard workflow includes:

(1) Low-speed centrifugation (300×g): to remove cell debris and large impurities.

(2) Medium-speed centrifugation (1,000–20,000×g): to eliminate larger extracellular vesicles, including apoptotic bodies and large microvesicles.

(3) High-speed ultracentrifugation (100,000×g): to efficiently pellet exosomes and small microvesicles.

 

Despite being labor-intensive and time-consuming, ultracentrifugation remains a highly effective and well-validated method for isolating high-quality exosomes from complex biological fluids.

 



Liu, W. Z. et al. Anal Bioanal Chem. 2022.

Figure 1. Schematic Diagram of Differential Ultracentrifugation-Based Exosome Purification

 

2. Microfiltration

Microfiltration utilizes membranes with defined pore sizes to physically remove large particles and aggregates. Serving as a critical complementary step to ultracentrifugation, microfiltration enhances sample clarity, reduces co-purification of non-exosomal proteins, and improves the overall purity and biological activity of exosomes. Hydrophilized polyvinylidene fluoride (PVDF) membranes are commonly employed to achieve optimal filtration across a variety of body fluid samples.

 



Davis, R. H. Current Trends and Future Developments on (Bio-) Membranes. 2019.

Figure 2. Schematic Diagram of Microfiltration-Based Exosome Purification

 

Service Advantages

Clients who choose MtoZ Biolabs' Ultracentrifugation and Microfiltration based Exosome Purification Service benefit from a fully optimized and professional exosome purification workflow supported by advanced equipment and an experienced technical team. This approach ensures high-quality, reproducible, and scalable results for both research and industrial applications. Key advantages include:

· High-purity exosome isolation: Efficient removal of non-exosomal particles for optimal sample purity.

· Improved purification efficiency: Streamlined workflow with reduced contaminants and higher reproducibility.

· Proven technical platform: Robust, well-validated purification system backed by years of expertise.

· Customized solutions: Tailored protocols to meet diverse research and application needs.

· High-throughput capacity: Suitable for both small-scale studies and large-scale production.

 

FAQ

Q1: Besides ultracentrifugation and microfiltration, what other methods are available for exosome purification?

In addition to ultracentrifugation and microfiltration, MtoZ Biolabs offers advanced purification techniques such as density gradient centrifugation, affinity chromatography, and immunoaffinity capture. Density gradient centrifugation effectively separates exosomes from other vesicles and contaminants, while affinity chromatography and immunoaffinity capture utilize specific antibodies to selectively enrich exosomes, providing highly specific and pure exosome preparations. These methods can be applied flexibly depending on sample type and research objectives.

 

Q2: How is exosome purity assessed?

To ensure the highest quality, MtoZ Biolabs employs a combination of state-of-the-art characterization techniques:

· Nanoparticle Tracking Analysis (NTA): for precise measurement of exosome size distribution and concentration.

· Transmission Electron Microscopy (TEM): for detailed visualization of exosome morphology and confirmation of structural integrity.

· Western Blot or ELISA: to detect and quantify exosome-specific markers such as CD9, CD63, and CD81, confirming the identity and purity of isolated exosomes.

This multi-layered validation strategy guarantees that all delivered exosome samples meet rigorous quality standards suitable for advanced research applications.

 

MtoZ Biolabs' Ultracentrifugation and Microfiltration based Exosome Purification Service provides a high-efficiency, precise solution for isolating high-purity exosomes to support diverse research and clinical applications, including biomarker discovery, disease mechanism studies, and therapeutic development. For detailed consultation and customized solutions, please feel free to contact us.