Exosome Separation & Purification Service
- Removal of cellular debris and apoptotic bodies: Low-speed centrifugation + 0.22 μm filtration.
- Concentration of large-volume samples (e.g., ultrafiltration centrifugation) to enhance downstream efficiency.
- Primary Enrichment: Initial capture via ultracentrifugation (100,000×g) or PEG precipitation.
- High-Precision Purification: Advanced removal of contaminants (e.g., lipoproteins) using IEC or SEC.
- Physical Characterization: NTA (size distribution and concentration), TEM (morphological verification).
- Biochemical Marker Detection: Western Blot/ELISA for exosome surface markers (CD9/CD63/TSG101).
- Functional Validation (Optional): e.g., scratch assay to assess exosome-induced wound healing activity.
- Liquid Biopsy: High-purity exosome isolation enables reliable detection of circulating tumor DNA/RNA for early cancer screening.
- Cell Therapy Optimization: Purified mesenchymal stem cell (MSC)-derived exosomes for anti-inflammatory and tissue repair drug development.
- Disease Mechanism Studies: Exosome isolation from cerebrospinal fluid (e.g., Parkinson’s or Alzheimer’s studies) to uncover disease-associated biomarkers.
Exosomes, critical mediators of intercellular communication, carry proteins, nucleic acids, and lipids from their parent cells, demonstrating immense potential in disease diagnosis, prognosis evaluation, and targeted therapy. However, the efficient isolation and high-purity purification of exosomes remain major technical barriers to their clinical application.
Services at MtoZ Biolabs
MtoZ Biolabs’ Exosome Separation & Purification Service integrates advanced methodologies to provide tailored solutions for isolating intact exosomes from complex biological samples (e.g., blood, cell culture supernatant, urine). Through differentiated separation strategies (combining traditional and emerging techniques) and stringent quality control, our service ensures optimized exosome yield, purity, and bioactivity, laying a reliable foundation for downstream functional studies, drug development, and clinical translation.
MtoZ Biolabs offers Exosome Separation & Purification Service designed to deliver high-yield, high-purity exosome preparations using advanced methods customized for diverse sample types and research objectives. Our services include:
1. Multi-Sample Compatibility: Supports exosome isolation from serum, plasma, cell culture medium, cerebrospinal fluid, urine, and more.
2. Flexible Methodological Framework: Utilizes ultracentrifugation (UC), density gradient centrifugation, ion-exchange chromatography (IEC), size-exclusion chromatography (SEC), polymer-based precipitation (e.g., PEG), and microfluidic chip technology, depending on client needs.
3. End-to-End Quality Control: Validates exosome integrity and functionality through nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), Western Blot (CD9/CD63/CD81 marker detection), and functional assays (e.g., cell uptake experiments).
Analysis Workflow
1. Sample Preprocessing
2. Core Isolation Steps
3. Purity and Integrity Validation
Chen, J. et al. Front Bioeng Biotechnol. 2022.
Figure 1. Schematic Representation of Common Exosomal Separation Techniques
Why Choose MtoZ Biolabs?
1. Guaranteed High Purity
Incorporates ion-exchange chromatography (IEC) to minimize protein contamination (<5% residual impurities), achieving 30% higher purity than traditional UC methods.
2. High Recovery Rate & Bioactivity Retention
Optimized protocols preserve membrane integrity, ensuring reliability for downstream RNA/proteomic analysis; functional validation supports therapeutic efficacy evaluation.
3. Flexibility and Scalability
Customizable solutions for micro-volume clinical samples (100 μL serum) to industrial-scale production (e.g., serum-free culture medium).
4. Rigorous Quality Control
ISO 9001-certified workflows with triple validation (physical + biochemical + functional) before delivery.
Applications
Deliverables
1. Exosome suspensions (cryopreserved or liquid form in PBS buffer)
2. Comprehensive QC report (NTA profiles, Western Blot results, TEM images)
3. Exosome quantification (BCA protein concentration analysis)
4. Exosomal RNA/protein extraction and sequencing (via partner collaboration)
In the era of "exosome-driven science," MtoZ Biolabs combines technical innovation and stringent quality control to deliver end-to-end exosome separation and purification services. Whether for basic research or clinical translation, we commit to providing high-purity, bioactive, and consistent exosome preparations to overcome experimental challenges and accelerate breakthroughs. Contact us today and let MtoZ Biolabs become your trusted partner in exosome research!
How to order?
