Subcellular Analysis Services
Subcellular structures refer to the functional units within a cell, such as mitochondria, endoplasmic reticulum, nuclei, lysosomes, and Golgi apparatus. These organelles play a central role in maintaining cellular functions, executing metabolic activities, regulating signal transduction, and supporting cell growth and division. The proteins within each organelle have unique distributions and functions. Studying the composition, interactions, and dynamic changes of these proteins is essential for understanding cellular life activities, exploring disease mechanisms, and developing therapeutic targets.
Subcellular analysis involves systematically analyzing proteins, metabolites, lipids, and other biomolecules from various cellular structures (organelles) using high-throughput techniques. Through organelle isolation and identification, along with quantitative and post-translational modification (PTM) analysis, subcellular analysis helps to deepen our understanding of cellular functions and the changes that occur in different physiological and pathological states.
MtoZ Biolabs offers comprehensive subcellular analysis services with the support of advanced mass spectrometry platforms and mature organelle separation technologies, providing efficient support for your research.
Services at MtoZ Biolabs
MtoZ Biolabs' Subcellular Analysis Service is designed to help researchers comprehensively analyze the protein composition, expression patterns, post-translational modifications, and more in cellular organelles using high-resolution mass spectrometry technology combined with organelle separation strategies. This service supports research in areas such as disease studies, drug development, immunology, and cell biology. Our Subcellular Analysis Services include:
Leveraging cutting-edge multi-omics platforms, robust bioinformatics pipelines, and stringent quality control systems, MtoZ Biolabs has established a comprehensive Exosome Analysis Services covering exosome separation and purification, exosome profiling, exosome characterization, exosomics analysis, and more, offering an all-in-one solution for researchers and biotech innovators worldwide.
2. Extracellular Vesicle Profiling Services
Extracellular vesicle profiling services are widely used in the basic research and clinical translation of cancer, neurodegenerative diseases, metabolic diseases, cardiovascular diseases, immune diseases, and more. By systematically analyzing the molecular characteristics of EVs, researchers can screen disease-related biomarkers, explore disease mechanisms, and promote the development of novel diagnostic tools and therapeutic strategies, particularly in cutting-edge fields such as liquid biopsy, biomarker discovery, and precision medicine.
3. Membrane Proteomics Service
MtoZ Biolabs employs Thermo Fisher's Orbitrap Exploris 240 mass spectrometer platform combined with Nano-LC chromatography to provide comprehensive subcellular proteomics analysis services, including qualitative and quantitative analysis of cellular, mitochondrial, and chloroplast membrane proteomes.
4. Endoplasmic Reticulum Analysis Services
Endoplasmic Reticulum Analysis Services are comprehensive research solutions centered on the functional and compositional characteristics of the endoplasmic reticulum (ER). These services encompass ER isolation and purification, membrane protein extraction, proteomic profiling, and metabolomic analysis. By integrating high-purity organelle enrichment techniques, high-resolution mass spectrometry platforms, and multidimensional bioinformatics analysis, these services enable systematic exploration of the ER’s molecular composition, functional status, and potential associations with disease under specific physiological or pathological conditions.
5. Chloroplast Analysis Services
MtoZ Biolabs offers high-quality Chloroplast Analysis Services powered by high-resolution mass spectrometry platforms and advanced bioinformatics. Covering both chloroplast proteomics and metabolomics, our Chloroplast Analysis Services are applicable to a wide range of plant species and experimental conditions. We help researchers uncover dynamic regulatory networks in chloroplasts, accelerating discoveries in plant biology, crop improvement, and environmental stress research.
At MtoZ Biolabs, we have developed a dedicated nuclear omics platform that combines state-of-the-art instrumentation with expert bioinformatics, offering end-to-end Nuclear Analysis Services to support both basic research and translational applications.
7. Peroxisome Analysis Services
MtoZ Biolabs offers comprehensive Peroxisome Analysis Services that integrate high-resolution mass spectrometry with multi-omics strategies such as proteomics, metabolomics and lipidomics and are further supported by advanced bioinformatics. These services enable in-depth molecular investigation of peroxisome biology under both physiological and pathological conditions, driving discoveries in systems cell biology, precision medicine and metabolic research.
8. Golgi Apparatus Analysis Services
MtoZ Biolabs offers comprehensive Golgi Apparatus Analysis Services that integrate advanced organelle isolation strategies with high-resolution mass spectrometry and multi-omics workflows. Our services are designed to support discovery in cell biology, pathogenesis, and biomarker research.
9. Centrosome Analysis Services
MtoZ Biolabs delivers specialized Centrosome Analysis Services powered by advanced proteomics, metabolomics, and lipidomics platforms. We provide tailored, high-throughput solutions that enable researchers to dissect the molecular composition, functional states, and dynamic regulatory mechanisms of centrosomes under various biological contexts.
Analysis Workflow
Service Advantages
1. Diverse Organelle Support System
We offer comprehensive organelle isolation and purification technologies, supporting the extraction of various subcellular structures, including mitochondria, endoplasmic reticulum, nuclei, lysosomes, and more, enabling researchers to explore cellular functions from multiple perspectives.
2. High-Resolution Mass Spectrometry Platform
MtoZ Biolabs is equipped with advanced mass spectrometry instruments (such as Orbitrap Fusion Lumos, Q Exactive HF-X) combined with stable NanoLC systems, ensuring precise detection and quantification of low-abundance proteins and complex modifications.
3. Flexible Quantitative Analysis Methods
We support multiple quantitative strategies, including Label-free, TMT, and iTRAQ labeling, to meet the needs of various experimental designs. Whether it's drug treatment, time-series research, or phenotypic comparisons, we provide accurate quantitative analysis.
4. Accurate Post-translational Modification Analysis
Proteins in organelles often undergo various post-translational modifications. MtoZ Biolabs provides PTM analysis services to help you study phosphorylation, acetylation, and other modifications, identifying key regulatory sites and advancing the study of organelle functions and disease mechanisms.
5. Transparent Pricing
MtoZ Biolabs adheres to transparent pricing principles, clearly listing all service costs before project initiation, avoiding hidden fees. We offer efficient and reliable services to ensure project completion while meeting your budget requirements.
Applications
1. Disease Research
Subcellular analysis helps reveal protein expression changes in cancers, neurodegenerative diseases, and other conditions, providing data support for early diagnosis and treatment.
2. Drug Development
During drug development, analyzing the impact of drugs on organelles helps assess their targeting and efficacy, optimizing drug design.
3. Cell Biology Research
Subcellular analysis helps study organelles and functional regions within cells, providing deeper insights into their roles during the cell lifecycle.
4. Immunology Research
By analyzing organelles within immune cells, subcellular analysis helps explore immune response, inflammation, and immune evasion mechanisms, supporting the development of immunotherapies.
FAQs
Q1: Can I choose only protein identification without quantitative analysis?
A1: Yes, we offer flexible services. You can select protein identification, quantitative analysis, or PTM analysis based on your research needs.
Q2: Do you support customized bioinformatics reports?
A2: Yes, we provide customized bioinformatics reports tailored to your research goals, including interactive visual maps.
Sample Submission Suggestions
1. Sample Types: Cell cultures, tissue homogenates, or isolated organelles.
2. Recommended Quantity: At least 3 samples per group, with protein concentration >1 µg/µL and total amount >50 µg.
3. Sample Storage: Store at -80°C, ship with dry ice, and avoid freeze-thaw cycles.
4. Special Requirements: If performing quantitative or PTM analysis, please communicate experimental conditions and preprocessing requirements in advance.
Deliverables
1. Complete raw mass spectrometry data (RAW format)
2. Protein identification and quantitative results (Excel format)
3. Enrichment analysis and functional annotation reports (PDF or interactive maps)
4. Visual maps: GO/KEGG enrichment plots, heat maps, volcano plots, PPI networks
5. Project summary report and methodology documentation
MtoZ Biolabs’ Subcellular Analysis Service provides powerful data support for cell function studies, disease mechanism analysis, and drug development. Through our services, you will gain in-depth understanding of the dynamic and functional roles of proteins within organelles, advancing your research. If you are conducting related studies, feel free to contact us for a customized analysis plan and quotation to support breakthroughs in your research.
Contact us for a free technical evaluation and quote!
How to order?
