Endoplasmic Reticulum Analysis Services
Endoplasmic Reticulum Analysis Services are comprehensive research solutions centered on the functional and compositional characteristics of the endoplasmic reticulum (ER). These services encompass ER isolation and purification, membrane protein extraction, proteomic profiling, and metabolomic analysis. By integrating high-purity organelle enrichment techniques, high-resolution mass spectrometry platforms, and multidimensional bioinformatics analysis, these services enable systematic exploration of the ER’s molecular composition, functional status, and potential associations with disease under specific physiological or pathological conditions.
Cao T. et al. Appl Microbiol Biotechnol. 2021.
The endoplasmic reticulum (ER) is one of the most structurally complex and functionally diverse organelles in eukaryotic cells, playing essential roles in protein synthesis and folding, lipid metabolism, calcium storage, and signal transduction. It is indispensable in responding to cellular stress, maintaining proteostasis, and regulating cell fate decisions such as apoptosis. ER dysfunction has been implicated in a wide range of diseases, including neurodegenerative disorders, metabolic syndrome, cardiovascular diseases, cancer, and viral infections. Therefore, comprehensive investigation of the ER's proteome, metabolome, and functional state is of great significance for uncovering disease mechanisms and developing novel targeted therapeutic strategies.
By leveraging proteomics, metabolomics, and other cutting-edge technologies, MtoZ Biolabs offers Endoplasmic Reticulum Analysis Services to systematically explore functional changes, metabolic characteristics, and signaling regulation of the ER under various cellular states and disease models. Our services provide robust data support for uncovering organelle-level biological insights and identifying new therapeutic targets.
Analysis Workflow
The main workflow of Endoplasmic Reticulum Analysis Services is as follows:
1. ER Isolation and Purification
Employ differential and density gradient centrifugation, combined with ER-specific markers for purity validation, to obtain intact and high-quality ER fractions.
2. Sample Preprocessing and Enrichment
Perform membrane protein extraction, metabolite isolation, or peptide preparation based on project requirements.
3. Analytical Detection
Conduct proteomic or metabolomic analysis, supporting qualitative profiling, quantitative measurement, and post-translational modification identification.
4. Multidimensional Bioinformatics Analysis
Deliver in-depth analysis including pathway enrichment, functional annotation, protein interaction networks, and metabolic pathway reconstruction.
Service Advantages
1. High-Purity ER Isolation: Utilizes an optimized centrifugation system combined with marker-based validation to achieve efficient isolation of the endoplasmic reticulum from tissues or cells.
2. Advanced Analysis Platform: MtoZ Biolabs established an advanced Endoplasmic Reticulum Analysis Services platform, guaranteeing reliable, fast, and highly accurate analysis service.
3. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
4. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all Endoplasmic Reticulum Analysis data, providing clients with a comprehensive data report.
5. Customizable Analytical Strategies: Offers tailored solutions to meet diverse research objectives, such as stress response analysis, metabolic profiling, and post-translational modification studies.
Services at MtoZ Biolabs
Endoplasmic Reticulum Analysis Services provided by MtoZ Biolabs include:
Endoplasmic Reticulum Isolation and ER Membrane Protein Purification Service
Efficiently isolates high-purity endoplasmic reticulum and its membrane proteins using optimized differential and density gradient centrifugation techniques.
Endoplasmic Reticulum Proteomics Services
Applies LC-MS/MS, TMT labeling, and PTM enrichment approaches to profile ER-associated protein expression and post-translational modifications.
Endoplasmic Reticulum Metabolomics Analysis Services
Employs platforms such as GC-MS and LC-MS to quantitatively analyze ER-related metabolites, including lipids, carbohydrates, and small signaling molecules.
Sample Submission Suggestions
Sample Types: Compatible with tissue samples, cell lines, and subcellular fraction extracts.
Storage and Shipping: Samples should be flash-frozen in liquid nitrogen and stored at −80°C. Avoid repeated freeze-thaw cycles. Ship using dry ice.
Additional Notes: Please contact our technical support team prior to submission for detailed and customized sample preparation guidance.
Applications
Application examples of Endoplasmic Reticulum Analysis Services:
ER Stress and Unfolded Protein Response (UPR) Mechanism Research
Investigate the regulatory role of ER stress and the UPR in disease progression.
Lipid Metabolism and Synthesis Studies
Explore metabolic pathways involving the synthesis and transport of lipids such as phospholipids and cholesterol within the ER.
Monitoring of Protein Folding and Modifications
Analyze post-translational modifications in the ER (e.g., glycosylation, acetylation, ubiquitination) and their roles in protein processing.
Disease Mechanism Investigation
Support mechanistic studies in models of neurodegenerative diseases, metabolic disorders, cancer, and more.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
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