Endoplasmic Reticulum Metabolomics Analysis Services
Endoplasmic Reticulum Metabolomics Analysis Services refers to a comprehensive service for metabolite extraction, identification, and quantitative analysis of endoplasmic reticulum (ER) subcellular components. This service focuses on analyzing the metabolic network within the ER at the subcellular level, using both targeted and untargeted metabolomics approaches to reveal the role of the ER in metabolic homeostasis and stress response.
Ventura R. Isabel Hernández-Alvarez M. Endoplasmic Reticulum: A Hub in Lipid Homeostasis. 2023.
ER is an organelle in eukaryotic cells responsible for core functions such as protein folding, lipid synthesis, and calcium ion storage. It is also a crucial hub for sensing and responding to cellular stress. With the advancement of systems biology, the metabolic state of the ER has been recognized as being closely associated with various diseases, such as cancer, metabolic syndrome, and neurodegenerative diseases. To better understand the metabolic role of the ER in maintaining cellular homeostasis and disease development, conducting metabolomic analysis at the subcellular level has become a key strategy.
Based on advanced chromatography and mass spectrometry platforms, MtoZ Biolabs offers Endoplasmic Reticulum Metabolomics Analysis Services providing high-sensitivity identification and quantitative analysis of metabolites in the ER subcellular structures. The service includes high-purity ER extraction, precise metabolite measurement, pathway enrichment analysis, and multidimensional data interpretation, helping researchers systematically explore the critical role of the ER in cellular metabolic regulation, stress response, and disease mechanisms.
Analysis Workflow
The general workflow of Endoplasmic Reticulum Metabolomics Analysis Services is as follows:
1. Sample Preparation and ER Separation
Extract high-purity ER fractions from cells or tissues.
2. Metabolite Extraction
Use a low-temperature organic solvent system to extract metabolites from the ER.
3. Detection and Data Acquisition
Select LC-MS, GC-MS, or NMR for qualitative and quantitative analysis based on the project goals.
4. Data Preprocessing
Includes quality control, peak recognition, alignment, and normalization.
5. Data and Bioinformatics Analysis
Analyze significant metabolic changes, perform pathway enrichment, and function analysis.
6. Result Integration and Report Output
Provide a comprehensive project report and visualized analytical charts.
Service Advantages
1. Advanced Analysis Platform: MtoZ Biolabs established an advanced Endoplasmic Reticulum Metabolomics Analysis Services platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all Endoplasmic Reticulum Metabolomics Analysis data, providing clients with a comprehensive data report.
4. High Purity ER Isolation: Utilize optimized differential and density gradient centrifugation protocols to ensure the purity and integrity of the target organelle components.
5. Dual Strategy Analysis Support: Offers both untargeted and targeted approaches, providing flexibility to meet various research needs.
Sample Submission Suggestions
Sample Types: Compatible with various biological materials including cell lines, primary cells, and tissue specimens.
Storage and Shipping: Immediately snap-freeze samples in liquid nitrogen after processing and store at −80°C. Avoid repeated freeze-thaw cycles. Ship samples on dry ice.
Additional Notes: Please contact our technical support team prior to submission for detailed and project-specific sample preparation guidelines.
Applications
Application Examples of Endoplasmic Reticulum Metabolomics Analysis Services:
Endoplasmic Reticulum Stress Research
Analyze metabolic changes in the ER during stress responses.
Lipid Metabolism Regulation Analysis
Investigate the role of the ER in lipid synthesis, transport, and metabolism.
Metabolic Interactions Between the ER and Other Organelles
Elucidate the metabolic coordination and interplay between the ER and organelles such as mitochondria, endosomes, and the Golgi apparatus.
Disease Mechanism Elucidation
Study disease mechanisms associated with ER dysfunction.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
Related Services
Endoplasmic Reticulum Proteomics Services
Endoplasmic Reticulum Isolation and ER Membrane Protein Purification Service
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