Endoplasmic Reticulum Isolation and ER Membrane Protein Purification Service
- Functional and structural studies of ER membrane proteins.
- Investigation of ER involvement in signal transduction pathways.
- Research on ER stress and apoptosis-related signaling pathways.
- Drug target screening and studies on membrane protein–drug interactions.
- Mechanistic studies of diseases associated with protein misfolding.
Endoplasmic Reticulum Isolation and ER Membrane Protein Purification Service uses advanced subcellular fractionation technology to efficiently and purify endoplasmic reticulum structures from cell or tissue samples, and further uses a mild and efficient elution method to extract functionally active membrane protein populations.
The endoplasmic reticulum (ER) is one of the most critical organelles in eukaryotic cells, playing essential roles in protein folding and modification, lipid synthesis, calcium storage, and detoxification. In particular, ER membrane proteins are key molecules involved in signal transduction, molecular transport, and membrane structural integrity. They are also frequently implicated as disease-associated targets within pharmacological pathways. In-depth characterization of ER components, especially membrane proteins, is vital for understanding cellular homeostasis, ER stress responses, and the molecular mechanisms of various diseases, including neurodegenerative disorders, metabolic syndromes, and cancers.
By integrating density gradient centrifugation, differential centrifugation, and optimized buffer systems, MtoZ Biolabs provides Endoplasmic Reticulum Isolation and ER Membrane Protein Purification Service that ensures the recovery of structurally intact and highly purified ER fractions and associated membrane proteins. This service supports ER isolation from diverse sample types and can be further extended to include protein concentration, quantification, and mass spectrometry analysis, enabling clients to systematically investigate ER functions, identify potential target proteins, and advance disease mechanism research and drug discovery.
Analysis Workflow
Wang X. et al. eLife. 2017.
Figure 1. Schematic diagram of tubular ER isolation.
The main steps of Endoplasmic Reticulum Isolation and ER Membrane Protein Purification Service are as follows:
1. Sample Preprocessing
Preparation of cell or tissue homogenates to release organelles.
2. Differential Centrifugation
Removal of non-target components such as nuclei and mitochondria to obtain microsomal fractions.
3. Density Gradient Separation
Use of sucrose density gradient centrifugation to further separate rough and smooth ER fractions.
4. Membrane Protein Extraction and Concentration
Extraction of membrane proteins under mild conditions, followed by concentration using ultrafiltration or protein precipitation.
5. Purity Validation and Quantification
Confirmation of ER and membrane protein purity using Western blot (WB), SDS-PAGE, and mass spectrometry.
Service Advantages
1. High-Purity ER Isolation: Combines multi-step centrifugation and gradient separation to minimize cross-contamination from mitochondria, plasma membrane, and other organelles.
2. Preservation of Native Protein Conformation: Membrane proteins are extracted under non-denaturing conditions, enabling compatibility with downstream functional assays and mass spectrometry analysis.
3. Experienced and Quality-Controlled Workflow: Extensive expertise in organelle isolation and membrane protein handling, with built-in quality validation steps to ensure research-grade deliverables.
4. Strong Downstream Compatibility: Isolated ER fractions are directly compatible with SDS-PAGE, Western blotting, LC-MS/MS, and structural analysis.
Sample Submission Suggestions
Sample Types: Compatible with a wide range of samples, including animal tissues, cultured cells, yeast, and plant cells.
Storage and Shipping: Samples should be flash-frozen in liquid nitrogen immediately after processing and stored at −80°C. Avoid repeated freeze-thaw cycles. Ship samples on dry ice.
Additional Notes: Please contact our technical support team prior to submission for detailed and tailored sample preparation guidance.
Applications
Endoplasmic Reticulum Isolation and ER Membrane Protein Purification Service is applicable to a variety of basic and translational research needs, including:
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. ER Microsomes/Membrane Protein Extracts (Available in Lyophilized or Liquid form Upon Request)
4. Sample Purity Validation Report (Western Blotting/Image Data/SDS-PAGE)
5. Optional Mass Spectrometry Analysis and Other Reports
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