Nanoparticle Tracking Analysis-based Exosome Characterization Service

Nanoparticle tracking analysis-based exosome characterization is an exosome characterization service based on nanoparticle tracking analysis technology, which is dedicated to detecting and analyzing the size distribution, concentration and dynamic changes of nanoscale vesicles such as exosomes. Exosomes are small vesicles secreted by cells that have important biological functions, such as intercellular information transmission and material exchange. Nanoparticle tracking analysis (NTA) uses the principle of light scattering and Brownian motion to track the dynamic behavior of particles, and can efficiently provide detailed information such as the size distribution, concentration and morphology of exosomes.

 

Nanoparticle tracking analysis-based exosome characterization service is widely used in the field of biomedical research, especially in oncology, neuroscience, and immunology. Nanoparticle tracking analysis (NTA) can be used for quantitative analysis of exosomes, helping researchers reveal disease markers, optimize drug delivery systems, and evaluate the potential of exosomes in disease diagnosis and treatment. It is also commonly used for quality control and standardization of exosomes to support clinical research and biotechnology product development.

 



Sharma, V. et al. Translational Neurodegeneration, 2023.

Figure 1. Schematic Representation of General Characteristics of Exosomes and their Characterization Via NTA.

 

Services at MtoZ Biolabs

Based on advanced nanoparticle tracking analysis (NTA) instruments, the nanoparticle tracking analysis-based exosome characterization service provided by MtoZ Biolabs can measure the particle size distribution and concentration with high precision. Through real-time tracking of laser scattering and Brownian motion, the particle size range, average particle size, distribution curve and particle concentration data of exosomes are accurately obtained to ensure that the results have high sensitivity and high resolution. These data are of great value for studying the biological characteristics of exosomes, optimizing drug delivery systems and discovering disease markers.

 

Service Advantages

1. High Resolution and High Precision

Nanoparticle tracking analysis can accurately measure the particle size and concentration of exosomes, especially for the characterization of low-abundance samples. By tracking the movement trajectory of nanoparticles in real time, it provides a detailed analysis of the particle size distribution of exosomes, ensuring high resolution and high accuracy of the data.

 

2. Versatility and Diversity

Not only can it be used for quantitative analysis of particle size distribution and concentration, but it can also provide support for the study of exosome surface characteristics. It can adapt to different sources of exosomes, including cell culture supernatant, blood, urine and other biological fluid samples.

 

3. High Throughput and High Efficiency

It is capable of analyzing large-scale samples, with high sample processing speed and data analysis efficiency. A large amount of accurate data can be obtained in a short time, meeting the needs of high-throughput analysis.

 

4. Customized Analysis Solutions

MtoZ Biolabs can provide personalized analysis services based on the customer's research goals and needs to ensure the most valuable metabolite data for specific research directions.

 

Applications

1. Cancer Research and Biomarker Discovery

Nanoparticle tracking analysis can accurately measure the size and concentration of exosomes in tumor patients, helping to identify exosome characteristics related to cancer occurrence, metastasis and immune escape, thereby providing data support for early diagnosis, monitoring and discovery of therapeutic targets for cancer.

 

2. Drug Delivery and Gene Therapy

Nanoparticle tracking analysis-based exosome characterization service can be used to characterize engineered exosomes, analyze their drug loading capacity and stability, optimize drug delivery efficiency, and improve treatment effects. At the same time, exosomes carrying genetic materials such as siRNA and mRNA can be analyzed to evaluate their potential for application in gene therapy.

 

3. Metabolic Disease Research

In the study of metabolic diseases such as diabetes and obesity, nanoparticle tracking analysis technology can help analyze the role of exosomes in lipid metabolism and sugar metabolism, providing support for revealing the mechanisms of metabolic disorders and developing new treatment strategies.

 

4. Intercellular Communication Research

Nanoparticle tracking analysis-based exosome characterization service is used to study the role of exosomes in intercellular information transmission, which helps to understand its mechanisms in immune regulation, cell signaling, etc.

 

Case Study

1. Characterization of Exosomes Derived from Ovarian Cancer Cells and Normal Ovarian Epithelial Cells by Nanoparticle Tracking Analysis

The study of this article aims to characterize the exosomes secreted by ovarian cancer cells and normal ovarian epithelial cells by nanoparticle tracking analysis (NTA) technology, and explore the differences in exosome characteristics between the two. The research subjects are exosomes secreted by ovarian cancer cells and normal ovarian epithelial cells. The purpose is to compare the characteristic differences of exosomes from ovarian cancer and normal cells by quantitatively analyzing the size and concentration of exosomes. The research method uses NTA to measure the particle size distribution and concentration of exosomes, and analyzes the changes in size, concentration and distribution of exosomes secreted by the two groups of cells. The results showed that there was a significant difference in the particle size distribution of exosomes derived from ovarian cancer cells and exosomes derived from normal ovarian cells. The concentration of ovarian cancer exosomes is generally higher and the particle size is smaller. In conclusion, NTA can effectively distinguish exosomes secreted by ovarian cancer cells and normal ovarian cells, and reveals the differences in size and concentration, which may be of great significance to the discovery of biomarkers and early diagnosis research of ovarian cancer.

 



Zhang, W. et al. Tumor Biology, 2016.

Figure 2. Sizes and Size Distributions of the Four Types of Exosomes as Analyzed by NTA for HOSEPiC.

 

FAQ

Q1: How to Ensure the Accuracy of Exosome Characterization?

A1: This service uses high-precision nanoparticle tracking analysis equipment, combined with standardized sample preparation and analysis processes to ensure data accuracy. Through multiple measurements and internal controls, the size distribution and concentration measurements of exosomes are ensured to be highly reproducible and consistent.

 

Q2: Are Data Analysis and Reporting Services Provided?

A2: Yes, the exosome characterization service based on nanoparticle tracking analysis not only provides particle size distribution and concentration data, but also includes detailed functional analysis reports to help customers understand the potential of exosomes in biological research and clinical applications.