Disease-Targeted Exosome Modification Service

Exosomes are membrane-bound nanovesicles secreted by cells, typically ranging from 30-150 nm in diameter. Recently, exosomes have emerged as a promising tool in drug delivery, disease diagnosis, and treatment due to their inherent stability, low immunogenicity, and unique targeting abilities. Particularly in precision medicine, exosomes can carry various bioactive molecules, such as proteins, nucleic acids, and lipids, offering great potential for targeted disease therapy. However, achieving precise and efficient exosome modification to enhance disease-specific targeting remains a significant challenge in research and clinical translation.

 

MtoZ Biolabs offers Disease-Targeted Exosome Modification Service that integrates advanced genetic and chemical modification strategies to enhance the targeting specificity and therapeutic efficacy of exosomes. Our customer-oriented service utilizes specific functionalization techniques on exosome surfaces, incorporating targeting molecules such as proteins, antibodies, peptides, and ligands, effectively increasing exosome accumulation and absorption in targeted tissues or disease sites. Our service includes but not limited to:：

1. Tumor and Cancer-Targeted Exosome Modification Service

2. Prostate Cancer-Targeted Exosome Modification Service

3. Breast Cancer-Targeted Exosome Modification Service

4. Colorectal Cancer-Targeted Exosome Modification Service

5. Lung Cancer-Targeted Exosome Modification Service

6. Ovarian Cancer-Targeted Exosome Modification Service

7. Tumor Cells-Targeted Exosome Modification Service

 

Analysis Workflow

1. Exosome Isolation and Purification

High-purity exosomes are obtained through ultracentrifugation, size exclusion chromatography (SEC), or ultrafiltration methods.

 

2. Design and Preparation of Targeting Molecules

Based on disease types and customer needs, suitable targeting molecules (e.g., specific antibodies, ligands, fusion proteins) are selected, synthesized, and validated.

 

3. Functionalization of Exosome Surfaces

Employing bioorthogonal chemistry, genetic engineering, and lipid insertion technologies, targeting molecules are stably and efficiently attached to exosome surfaces, enabling precise targeting to disease tissues.

 

4. Validation and Characterization of Modified Exosomes

High-resolution electron microscopy, nanoparticle size analysis, flow cytometry, and Western blotting are utilized to validate the efficiency and quality of the modifications.

 



Chen, H. et al. Front Bioeng Biotechnol. 2023.

Figure 1. The Production Process of Engineered Exosomes through a Schematic Diagram

 

Service Advantages

1. Customized Solutions: Tailored targeting strategies designed precisely to meet diverse research and clinical requirements.

2. High-Efficiency Targeting: Significantly enhanced specificity and delivery efficiency to disease sites, improving therapeutic outcomes.

3. Advanced Technology: Utilization of cutting-edge bioorthogonal chemistry and bioengineering techniques ensuring stable and reliable modifications.

4. Professional Validation System: Comprehensive quality control measures guarantee the activity and quality of modified exosomes.

 

Applications

1. Tumor-targeted therapy and diagnostics (e.g., breast cancer, lung cancer, glioblastoma)

2. Central nervous system diseases (e.g., Parkinson's disease, Alzheimer's disease)

3. Cardiovascular diseases (e.g., myocardial infarction, ischemia)

4. Inflammatory and autoimmune diseases (e.g., rheumatoid arthritis, lupus)

5. Tissue regeneration and repair (e.g., wound healing, cartilage regeneration)

 

Deliverables

1. Exosome isolation and purification

2. Specific targeting molecule design and synthesis (proteins, peptides, antibodies, ligands)

3. Exosome surface functionalization and engineering

4. Functional validation and quality control analysis

5. Biological function evaluation and in vitro/in vivo experiments

 

MtoZ Biolabs is committed to advancing precision medicine through sophisticated exosome modification technologies. With our professional team, state-of-the-art facilities, and extensive practical experience, we provide high-quality Disease-Targeted Exosome Modification Service to support your research, overcome current limitations, and facilitate clinical translation toward precision healthcare.