Exosome Proteomics Service

Exosomes are one of the common extracellular vesicles (EVs) with diameters ranging from 30 to 150 nm. They primarily originate from the invagination of lysosomal particles into multivesicular bodies, which are released into the extracellular matrix, such as tissues and various biological fluids including serum/plasma, cerebrospinal fluid, and urine (in vivo), after the outer membrane of the multivesicular body fuses with the cell membrane. Additionally, exosomes can also exist in vitro under certain conditions in culture media. EVs play an important role in various physiological processes, and compared to general EVs, the function of exosomes in the field of clinical biomarkers is still unclear. With the development of exosome separation technologies, interest in this field is growing, and it is anticipated that more detailed studies on the function and applications of exosomes in disease pathologies will emerge.

 

Exosomes primarily contain a complex mixture of proteins, lipids, RNA, etc. The content and function of exosomes from different biological fluids vary, and compared to other contents, proteins play a crucial role in biological functions. Therefore, proteomic analysis of exosomes from different biological samples is significant for the discovery of biomarkers and clinical diagnosis.

 

MtoZ Biolabs provides professional and comprehensive exosomal proteomics analysis services, capable of identifying and quantifying proteins in exosomes. By integrating bioinformatics techniques for the analysis of proteomic data, the detection of biomarkers can be achieved.

 



Sun, Y. et al.  Anal. Chim. Acta. 2017.

Figure 1. Saliva and Serum Exosome Proteomics Workflow (Y. Sun et al., 2017)

 

Service Advantages

1. One-stop Exosome Proteomics Analysis

We can complete the entire process including exosome separation to protein identification and quantification. It is possible to separate exosomes from various types of samples, including plasma/serum, cerebrospinal fluid, saliva, snot, urine, bile, and cell culture media.

 

2. Advanced Facilities and Optimized Operation Procedures

MtoZ Biolabs uses the currently highest resolution and sensitivity mass spectrometer, the newly introduced Obitrap Fusion Lumos by Thermo company, combined with nanoLC chromatography for exosomal proteomics analysis. MtoZ Biolabs has extensive experience in the field of exosome proteomics analysis and can provide customized services.

 

3. Biomarker Detection through Bioinformatics Analysis

Our professional bioinformatics analysts are proficient in using various bioinformatics analysis tools, providing in-depth analysis and data mining.