Exosome Engineering Services
- Targeted Drug Delivery: Deliver chemotherapeutics, peptides, or small molecules to specific tissues or cells
- Gene Therapy: Transport mRNA, siRNA, or miRNA for gene modulation
- Cancer Immunotherapy: Display tumor antigens or immunostimulatory molecules for vaccine development
- Regenerative Medicine: Promote tissue repair via targeted exosome delivery
- Isolated Exosomes: ≥200 μg total protein or ≥100 μL in PBS
- Plasma/Serum: ≥2 mL per sample
- Cell Culture Supernatants: ≥5 mL per sample
- Other Body Fluids: Please inquire for specific requirements
Exosomes are nano-sized extracellular vesicles naturally secreted by nearly all cell types, playing pivotal roles in intercellular communication through the transport of proteins, lipids, and nucleic acids. Due to their endogenous origin, biocompatibility, low immunogenicity, and ability to cross biological barriers, exosomes have emerged as highly promising candidates for therapeutic delivery systems and diagnostic applications.
However, native exosomes often lack the specificity or cargo loading efficiency required for targeted biomedical use. To address this limitation, exosome engineering has become an essential strategy to improve exosome targeting, cargo capacity, stability, and functionality. Exosome engineering involves the modification of exosomal membranes, cargo loading, or both—either during biogenesis (endogenous methods) or after isolation (exogenous methods).
Service at MtoZ Biolabs
MtoZ Biolabs offers advanced Exosome Engineering Services to meet the growing demand for customized and functionalized exosomes in research and therapeutic development. We combine state-of-the-art engineering technologies with a deep understanding of exosome biology to deliver high-performance vesicles tailored to your objectives. Clients only need to inform us of the desired surface markers and cargo molecules, and our expert team will take care of the rest—from strategy design to delivery of high-quality engineered exosomes. Our Exosome Engineering Services encompass the following key components:
1. Exosome Labeling and Tracking
We offer customizable labeling strategies to facilitate real-time monitoring and functional tracing of engineered exosomes:
💠Lipid labeling using fluorescent dyes or click-modified lipids
💠Protein surface labeling with affinity tags, biotin, or antibodies
💠RNA labeling via fluorophore-conjugated oligonucleotides or metabolic precursors
2. Cargo Loading
Exosome engineering encompasses a wide array of strategies that can be broadly classified into two categories. Each method has distinct advantages and is selected based on the intended application, cargo type (e.g., mRNA, siRNA, protein, small molecules), and exosome origin.
💠Endogenous Engineering: Modification of parent cells through genetic editing or metabolic labeling so they produce exosomes carrying desired proteins, RNAs, or targeting motifs during vesicle biogenesis.
💠Exogenous Engineering: Direct modification of isolated exosomes using techniques such as electroporation, sonication, extrusion, freeze–thaw cycling, click chemistry, or membrane fusion.
3. Engineered Exosome Production
We provide end-to-end solutions for scalable production of engineered exosomes:
💠Establishment of engineered cell lines for stable exosome secretion
💠High-purity exosome isolation and purification using ultracentrifugation, SEC, or immunoaffinity
💠Comprehensive quality control, including size distribution, morphology (TEM), marker validation (WB, flow cytometry), and functional assays
4. Cargo Loading Evaluation
To ensure engineering efficiency and functional relevance, we offer:
💠Quantitative assessment of cargo loading efficiency via LC-MS/MS or qPCR
💠Integrity and stability checks of loaded cargo under storage or physiological conditions
💠Functional delivery validation in cell-based or biochemical assays
Figure 1. Engineering of Exosomes
Analysis Workflow
1. Project Consultation: Define project goals, sample origin, and desired modifications
2. Design Strategy: Choose optimal engineering method (endogenous/exogenous)
3. Experimental Execution: Conduct engineering, loading, and/or surface modifications
4. Quality Control: Characterize size, concentration, marker expression, and cargo loading
5. Data Delivery: Provide detailed reports including engineering parameters and QC results
Service Advantages
✔️Customizable Modification Strategies
Flexible surface functionalization and cargo-loading methods tailored to specific applications, targets, and delivery routes.
✔️Rigorous Quality Control
Each batch of engineered exosomes undergoes strict QC, including size distribution, marker validation, and loading efficiency assessments.
✔️Multi-Omics Integration
Support for proteomics, transcriptomics, lipidomics, and metabolomics to validate exosome functionality and composition.
✔️End-to-End Technical Support
From project design and method selection to delivery of engineered exosomes and data reports, our expert team ensures smooth project execution.
Applications
Engineered exosomes have broad and expanding applications:
Sample Submission Suggestions
We accept the following sample types:
Samples should be stored at -80°C and shipped on dry ice. Avoid repeated freeze–thaw cycles. Pre-consultation is encouraged for custom design.
Deliverables
1. Purified engineered exosomes (optional delivery formats: lyophilized or in suspension)
2. Detailed technical report covering engineering strategy, methodology, QC results, and storage instructions
3. Supporting raw data from characterization assays (e.g., Western blot, NTA, LC-MS/MS)
4. Optional: downstream omics data, functional assay results, or scale-up documentation based on request
Exosome engineering is transforming the landscape of targeted therapy, diagnostics, and precision medicine. MtoZ Biolabs is committed to supporting innovation through high-quality, customizable exosome engineering services that meet the demands of modern biomedical research. Contact us today to discuss your project and request a customized proposal!
Related Services
Exosome Isolation and Purification Service
Exosome Cargo Pre-loading Service
Exosome In Vitro Functional Research Services
How to order?
