Exosome Isolation and Purification Service
Exosomes are small extracellular vesicles secreted by most cell types and found in a variety of biofluids. Rich in proteins, lipids, RNAs, and metabolites, they play a key role in intercellular communication and are widely studied for their potential in diagnostics, therapeutics, and biomarker discovery. However, the value of exosome research critically depends on the ability to isolate high-purity vesicles free from contaminants. Biological samples contain numerous interfering substances such as cell debris, protein aggregates, apoptotic bodies, and lipoproteins, which closely resemble exosomes in size and density. These impurities can compromise downstream applications like omics profiling, structural analysis, and functional assays. To address this challenge, MtoZ Biolabs offers a comprehensive Exosome Isolation and Purification Service designed to deliver high-purity exosomes suitable for a wide range of downstream applications, including omics profiling, structural analysis, and functional assays.
Service at MtoZ Biolabs
MtoZ Biolabs' Exosome Isolation and Purification Service employs cutting-edge platforms and multiple isolation technologies to ensure high purity, yield, and structural integrity of exosomes from various biological matrices. Our available methods include ultracentrifugation, polymer-based precipitation, microfluidic separation, field-flow fractionation, size exclusion chromatography (SEC), tangential flow filtration (TFF), ultrafiltration, and immunoaffinity capture. Each technique can be selected or combined based on the sample type, target application, and desired purity level.
Dilsiz, N. Transl Oncol. 2024.
Figure 1. Schematic Representation of Common Exosomal Isolation and Purification Techniques
In addition to isolation and purification, we offer comprehensive downstream services including exosome characterization, proteomic, lipidomic, and metabolomic profiling, as well as transcriptomic analysis, enabling researchers to obtain a full spectrum of functional and molecular insights. Our expert team will help you choose the most appropriate isolation strategy and provide end-to-end technical support, from method selection to exosome recovery, quality validation, and integrated omics analysis.
Analysis Workflow
1. Sample Assessment and Pre-processing
Biological matrices such as plasma, serum, urine, or culture supernatants are assessed for quality and pre-cleared by low-speed centrifugation to remove cells and large debris.
2. Exosome Isolation
Depending on the sample type and research goal, we apply appropriate isolation methods such as differential centrifugation, density gradient centrifugation, ultrafiltration, TFF, SEC, or microfluidics.
3. Purification and Concentration
Further purification is performed using gradient separation, filtration, or immunoaffinity capture to achieve high-purity exosome fractions.
4. Quality Control and Validation
Isolated exosomes are evaluated for size distribution, concentration, morphology (via TEM or NTA), and marker expression (e.g., CD9, CD63, CD81).
5. Optional Downstream Services
Upon request, samples can be submitted for exosome-based proteomics, lipidomics, metabolomics, or transcriptomics to enable in-depth functional analysis.
Service Advantages
☑️Comprehensive Platform Selection: Multiple isolation methods including ultracentrifugation, TFF, SEC, and immunoaffinity capture to meet diverse sample and research needs.
☑️High Purity and Structural Integrity: Optimized protocols ensure the recovery of intact, contaminant-free exosomes suitable for sensitive downstream analyses.
☑️Flexible and Customizable Workflow: Tailored strategies based on sample type, target application, and desired purity level.
☑️Experienced Technical Team: Skilled scientists provide end-to-end support, including method selection, troubleshooting, and data interpretation.
Applications
💠Biomarker Discovery: Enables accurate detection of exosome-associated disease markers in clinical samples.
💠Multi-Omics Analysis: Provides high-quality starting material for exosome proteomics, lipidomics, metabolomics, and transcriptomics.
💠Functional Studies: Supports exosome-based research in intercellular communication, immune modulation, and signaling pathways.
💠Therapeutic Development: Facilitates the production of pure exosomes for drug delivery and vaccine design.
💠Diagnostics and Liquid Biopsy: Improves the sensitivity and specificity of exosome-based diagnostic assays.
Sample Submission Suggestions
1. Sample Types
We accept various sample types, including serum/plasma, urine, saliva, CSF, cell culture, and other biological samples.
2. Storage and Shipping Conditions
Samples should be stored at -80℃ and shipped with dry ice. Avoid repeated freeze-thaw cycles.
*Note: If you have specific requirements or need guidance on sample preparation, please do not hesitate to contact us.
Deliverables
1. Comprehensive experimental details
2. Isolated and purified exosome samples
3. Quality control report (particle size, concentration, purity markers)
4. Electron microscopy images and NTA data (Optional)
5. Downstream-ready material for omics profiling or functional assays (Optional)
6. Customized report based on project requirements
Reliable exosome isolation is the cornerstone of downstream exosome research. With advanced technologies and a flexible service portfolio, MtoZ Biolabs ensures high-quality, contaminant-free exosomes tailored to your research needs. Free project evaluation, welcome to learn more details!
Related Services
Exosomes Identification Service
How to order?
