Exosomes Identification Service
Exosomes are small, membrane-bound extracellular vesicles (30-150 nm in diameter) actively secreted by cells. They carry a variety of functional molecules, including proteins, lipids, and nucleic acids, and are widely found in biological fluids such as blood, urine, saliva, and cerebrospinal fluid. As key mediators of intercellular communication, exosomes play critical roles in tumor progression, immune regulation, neurological disorders, cardiovascular disease, and regenerative medicine. Accurate and comprehensive identification of exosomes is essential for functional studies, omics-based analyses, drug delivery research, and the discovery of diagnostic biomarkers.
Zhang, Q. et al. Int J Nanomedicine. 2024.
Figure1. Structure and Contents of Exosome
Exosome identification involves a multi-level analysis encompassing physical properties, molecular markers, and functional contents:
1. Particle Size and Concentration Analysis
Nanoparticle Tracking Analysis (NTA) is used to dynamically measure the size distribution and particle concentration of exosomes, ensuring that vesicles fall within the typical 30-150 nm range.
2. Morphological Characterization
Transmission Electron Microscopy (TEM) provides direct visualization of exosome morphology, confirming their characteristic cup-shaped appearance and lipid bilayer structure.
3. Surface Marker Profiling
Classical exosomal surface proteins—such as CD9, CD63, CD81, TSG101, and Alix—are detected via Western blotting, flow cytometry, or immunodetection-based assays to verify vesicle identity.
4. Proteomic and RNA Content Analysis
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) and RNA sequencing are employed to analyze internal cargo, including proteins and small RNAs (e.g., miRNAs), offering insights into exosome origin and potential biological functions.
Saleem, M. et al. Stem Cell Res Ther. 2024.
Figure2. Different Techniques for Characterization of Exosomes
Service at MtoZ Biolabs
MtoZ Biolabs offers a comprehensive, one-stop exosome identification service powered by advanced analytical platforms, including high-resolution mass spectrometry, nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and flow cytometry. Our integrated workflow enables full-spectrum exosome validation, from morphological assessment to molecular profiling, ensuring the quality and reproducibility required for downstream research and translational applications.
We offer modular exosome identification service that can be provided individually or as part of a complete package. These services are tailored to meet a wide range of experimental needs:
✅ Particle Size and Concentration Measurement (NTA-based)
✅ Morphological Analysis (High-resolution TEM imaging)
✅ Surface Marker Detection (Western blot, flow cytometry, or ELISA)
✅ Proteomic Profiling (LC-MS/MS-based protein identification)
✅ RNA Content Analysis (miRNA, mRNA, via qPCR or RNA-Seq)
Why Choose MtoZ Biolabs
1. Multi-platform Integration:
Cross-validation using NTA, TEM, Western blotting, and flow cytometry minimizes false positives and sample contamination, ensuring robust and reliable exosome characterization.
2. High-sensitivity Mass Spectrometry Platform:
Advanced instruments such as the Orbitrap Fusion Lumos enable deep profiling and accurate quantification of exosomal proteins and peptides with high resolution and sensitivity.
3. One-Time-Charge:
Our pricing is transparent, no hidden fees or additional costs.
4. High-Data-Quality:
Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all exosomes identification service data, providing clients with a comprehensive data report.
Application
· Exosome Functional StudiesInvestigating the role of exosomes in intercellular communication and signal transduction.
· Biomarker DiscoveryIdentifying exosome-derived biomarkers associated with cancer, cardiovascular disease, and neurodegenerative disorders.
· Therapeutic Carrier DevelopmentEvaluating exosomes as delivery vehicles for therapeutic molecules such as miRNA, siRNA, and proteins.
· Pre-analytical Quality Control for OmicsAssessing sample integrity prior to downstream proteomic or transcriptomic analysis.
· Quality Control for Exosome-based ProductsSupporting the development and quality assessment of exosome reagents, diagnostics, and extracellular vesicle therapeutics.
Sample Submission Suggestions
*Other sample types, such as cerebrospinal fluid (CSF), amniotic fluid, bile, breast milk, tissue homogenates, and plant-derived fluids, are also supported. Please contact us for specific handling recommendations.
Deliverables
✔ Report on exosome particle size and concentration
✔ TEM images or flow cytometry profiles
✔ Protein and/or RNA analysis data (including raw files and statistical summaries)
✔ Surface marker expression results (e.g., Western blot images)
✔ Documentation of experimental procedures and analytical platforms used
Related Service
Exosomal Small RNA/miRNA Sequencing Service
Transmission Electron Microscope (TEM) based Exosome Characterization Service
Nanoparticle Tracking Analysis-based Exosome Characterization Service
How to order?
