Exosomal mRNA Microarray Service
- Biomarker Discovery: Identifying reliable biomarkers for early disease detection, prognosis, or therapeutic responses.
- Disease Mechanism Studies: Investigating molecular pathways and gene regulatory networks underlying various diseases, including cancer, neurodegenerative, cardiovascular, and autoimmune disorders.
- Drug Development and Therapeutic Evaluation: Monitoring treatment efficacy and molecular responses, accelerating personalized medicine and drug discovery.
- Clinical and Translational Research: Facilitating the transition from bench-side discoveries to clinical applications by providing precise molecular profiling data.
Exosomes are nanoscale extracellular vesicles actively secreted by most cell types and are present in various biological fluids. They encapsulate a rich cargo of bioactive molecules, including proteins, lipids, and nucleic acids. Exosomal mRNA plays a pivotal role in mediating intercellular communication by being translated into functional proteins in recipient cells, thereby influencing numerous biological processes such as immune response modulation, tumor progression, drug resistance, and tissue repair. The ability to analyze mRNA content in exosomes offers a non-invasive approach to gain insights into disease mechanisms, monitor treatment response, and discover novel biomarkers. High-throughput and sensitive detection of these transcripts is therefore critical for both basic and translational research. Recognizing this potential, MtoZ Biolabs offers an advanced Exosomal mRNA Microarray Service to precisely analyze mRNA expression patterns, providing valuable insights for biomedical research and clinical applications.
Exosomal mRNA microarray is a high-throughput and reliable technique for profiling mRNA expression within exosomes. The workflow begins with the isolation of exosomes from biological fluids or cell culture supernatants, followed by RNA extraction and enrichment of the mRNA fraction. The purified mRNAs are then reverse-transcribed to generate cDNA, which is hybridized to a microarray chip containing pre-labeled, gene-specific probes. Fluorescence signals generated from probe-target hybridization are scanned and quantified, enabling comprehensive analysis of exosomal transcriptomic patterns and identification of differentially expressed genes relevant to disease and therapy.
Source: https://en.m.wikipedia.org/wiki/Microarray_analysis_techniques#
Figure 1. The Steps Required in a Microarray Experiment
Service at MtoZ Biolabs
MtoZ Biolabs offers a specialized Exosomal mRNA Microarray Service, providing end-to-end solutions from exosome isolation to comprehensive transcriptomic profiling. We employ standardized protocols and high-efficiency isolation methods to ensure the integrity and purity of exosomal preparations. Total RNA extracted from exosomes undergoes rigorous quality assessment, followed by mRNA enrichment and labeling. Using leading microarray platforms, we enable sensitive hybridization and high-throughput detection of thousands of mRNA transcripts. Our integrated bioinformatics pipeline delivers reliable differential expression analysis, functional annotation, and pathway enrichment, helping clients uncover biologically meaningful patterns from exosomal mRNA data. Whether for disease biomarker discovery, therapeutic monitoring, or mechanistic studies, MtoZ Biolabs provides customizable and reproducible services tailored to your research needs. If you are interested in our Exosomal mRNA Microarray Service, please feel free to contact us. Our technical specialists are available to provide a free business assessment.
Service Advantages
☑️High Sensitivity with Low Input
Optimized workflows enable accurate detection of exosomal mRNA from minimal starting material, making it suitable for limited or precious samples.
☑️Standardized Exosome and RNA Processing
Rigorous protocols for exosome isolation and RNA extraction ensure sample consistency and data quality across batches.
☑️Advanced Microarray Platforms
Utilization of high-resolution and high-density microarray chips allows broad transcriptome coverage and reliable detection of expression changes.
☑️Integrated Bioinformatics Support
Comprehensive downstream analysis includes normalization, differential expression, pathway enrichment, and data visualization, providing deep biological insights.
☑️Customizable Solutions
Services can be tailored to specific research goals, including disease models, tissue types, or targeted transcript panels.
Applications
MtoZ Biolabs’ Exosomal mRNA Microarray Service is ideal for:
Case Study
Exosomal mRNA Microarray Reveals RNA-Mediated Communication Between Mast Cells and Progenitor Cells
A pioneering study investigated whether exosomes derived from human mast cells could mediate intercellular communication through RNA transfer. Using exosomes secreted by the HMC-1 human mast cell line, researchers employed both mRNA and microRNA microarray platforms to profile their RNA content. The analysis revealed approximately 1,800 distinct mRNAs and 116 microRNAs within the exosomal cargo, representing about 15% of the mRNA population in the donor cells. Functional assays, including co-culture, confocal microscopy, and radioactive labeling, confirmed that these RNA-containing exosomes were not only taken up by neighboring mast cells but also delivered their RNA payloads to CD34+ hematopoietic progenitor cells. This demonstrated that exosomal shuttle RNA (esRNA) serves as an active mediator of cell-to-cell communication, potentially influencing cell differentiation and maturation processes.
This study highlights the power of exosomal mRNA microarray analysis in uncovering transcriptomic signatures and validating the biological functions of extracellular RNA. At MtoZ Biolabs, our Exosomal mRNA Microarray Service supports similar investigations by offering high-throughput, sensitive, and reproducible analysis of exosomal mRNA profiles, enabling researchers to explore RNA-mediated signaling mechanisms and identify novel biomarkers in both physiological and disease contexts.
Figure 2. Characterization of Exosomal mRNA
FAQ
Q1: Do I need to isolate exosomes or RNA before submission?
Not necessarily. MtoZ Biolabs offers full workflow services including exosome isolation and RNA extraction. However, we also accept pre-isolated exosomes or RNA if clients prefer to perform those steps in-house.
Q2: Can you provide custom microarray panels for specific research needs?
Yes, we provide customizable microarray chip configurations to match your unique research requirements. Whether you're focusing on disease-associated gene panels or need probe sets designed for specific mRNA targets, our team can assist in developing a solution tailored to your project.
How to order?
