Exosome Profiling Service

Exosome Profiling Service systematically analyzes exosomes based on their physical characteristics and molecular markers, including particle size distribution, surface protein profiles, RNA composition, and functional features. As key mediators of intercellular communication, exosomes carry diverse biomolecules such as proteins, nucleic acids, and lipids, making them a "gold mine" for liquid biopsy in disease diagnosis, mechanism research, and drug development. However, the heterogeneity, low abundance, and complex backgrounds of exosomes pose great challenges for accurate analysis.  

 

Our Exosome Profiling Service leverages advanced exosome isolation technologies and multidimensional molecular profiling, combining ultracentrifugation, immunoaffinity capture, and nano-flow cytometry for high-purity separation, integrated with mass spectrometry, sequencing, and molecular imaging. We provide high-precision exosome subpopulation characterization, revealing dynamic changes in disease progression and treatment response, accelerating biomarker discovery and precision medicine strategies.   

√Comprehensive Analysis: From basic characterization (concentration, particle size) to in-depth molecular profiling (protein markers, miRNA, lncRNA), fully deciphering functional features.  

√Sample Flexibility: Applicable to plasma, serum, urine, cerebrospinal fluid, and cell culture media.  

√Low-Abundance Detection: Optimized workflows for trace exosome samples to ensure high recovery rates and low background interference.  

 



Wang, X. et al. Cancer Gene Ther. 2023.

Figure 1. Schematic Diagram of the Exosome Isolation Techniques

 

Analysis Workflow  

1. Sample Evaluation and Pre-processing  

• Free guidance on sample collection and preservation (e.g., centrifugation conditions, anticoagulant selection).  
• Stability testing to assess exosome integrity post-shipping.  

 

2. Exosome Isolation and Purification  

• Tailored choice of ultracentrifugation, polymer precipitation, or immunomagnetic capture based on sample type.  
• Ultrafiltration to remove free proteins and impurities, with exosome purity assessed via quantification kits.  

 

3. Phenotypic Analysis and Quality Control  

• Physical Characterization: Nano-particle tracking analysis (NanoFCM), transmission electron microscopy (TEM) to verify size and morphology.  
• Proteomics: Multiplex immunofluorescence and mass spectrometry targeting 50+ markers.  
• RNA Profiling: Small RNA-seq for miRNA and targeted mRNA/lncRNA panels.  
• Data Analysis: Differential expression screening, functional enrichment (KEGG/GO), and interaction network construction.  

 

4. Report Delivery and Interpretation  

• Standardized experimental report (raw data + visualization).  
• Complimentary one-on-one bioinformatics consultation for biological insight mining.  

 

Why Choose MtoZ Biolabs?  

1. Expert Team  

• Over 10 years of exosome research experience, handling 3,000+ samples covering 20+ disease models.  
• PhD-level team familiar with industry standards and cutting-edge technologies.  

 

2. Advanced Platforms  

• Equipped with ultracentrifuges, nano-flow cytometers, high-resolution mass spectrometers, ensuring accurate and reproducible data.  
• GLP-grade lab certified by ISO 9001 quality system.  

 

3. Flexible Collaboration Models  

• Customized Solutions: Tailored analysis combinations based on research goals (biomarker discovery/mechanism study), avoiding unnecessary costs.  
• Fast Turnaround: Standard delivery in 3-6 weeks (from sample receipt to final report), with expedited options available.  

 

4. One-Time-Charge

Transparent pricing without hidden fees.  

 

Applications  

Exosomes, as critical mediators of intercellular communication, offer vast applications in biomedical research and clinical translation. Our Exosome Profiling Service delivers systematic and high-sensitivity analysis for key areas:  

1. Disease Mechanism and Pathology Research  

• Microenvironment Analysis: Elucidate exosome-mediated signaling in disease progression (e.g., cancer, neurodegeneration, immune disorders).  
• Biomarker Discovery: Identify exosome-specific markers correlated with disease stages and prognosis, offering vital functional clues.  

 

2. Clinical Diagnosis and Health Monitoring  

• Non-invasive Liquid Biopsy: Develop early screening and disease classification tools from exosomal molecular profiles in blood and urine.  
• Therapeutic Monitoring: Track changes in exosome markers during treatment to assess drug response and resistance evolution.  

 

3. Drug Development and Therapeutic Exploration  

• Delivery Vehicle Design: Analyze natural exosome targeting properties to guide engineered drug carriers.  
• Target Validation: Identify and validate exosome-derived pathological targets to accelerate novel therapies.  

 

4. Precision and Personalized Medicine  

• Subpopulation Analysis: Characterize molecular diversity among patient-derived exosome subtypes to guide personalized therapies.  
• Prognostic Modeling: Integrate multi-omics data to build exosome-based prediction models for clinical outcomes and stratified patient management.  

 

Case Study

1. Profiling Surface Proteins on Individual Exosomes Using a Proximity Barcoding Assay

This study presents a novel proximity barcoding assay (PBA) for high-throughput profiling of surface proteins on individual exosomes. By combining antibody-DNA conjugates with next-generation sequencing, the method enables precise analysis of the protein composition on single exosomes, revealing their heterogeneity. The approach was first validated using artificial streptavidin-oligonucleotide complexes and then applied to exosomes isolated from human body fluids and cell culture media. The results demonstrated that exosomes from different sources exhibit specific combinations and abundances of surface proteins, allowing detailed profiling of exosome subpopulations and highlighting their potential as markers for tissue-specific processes in disease. Exosome Profiling Service offers barcode sequencing-based analysis of exosomal surface proteins, enabling high-throughput profiling of individual exosome protein composition to uncover their diverse origins. Applicable to various sample types, it supports deeper exploration of exosome heterogeneity and complex phenotypes.

 



Wu, D. et al. Nat Commun. 2019.

Figure 2. Bulk Protein Counts and Visualization of Individual Exosomes

 

2. Exosomes Multiplex Profling, a Promising Strategy for Early Diagnosis of Laryngeal Cancer

This study focuses on developing a minimally invasive method for early diagnosis of laryngeal cancer (LCa) through exosome profiling. Exosomes isolated from serum of 30 LCa patients and 20 healthy controls using a CD63-based affinity method were analyzed by multiplex flow cytometry to characterize membrane epitopes. The results revealed a distinct overexpression of markers such as CD1c, CD2, CD3, CD4, CD11c, CD14, CD20, CD44, CD56, CD105, CD146, and CD209 in LCa patients compared to healthy individuals. Additionally, CD24, CD31, and CD40 were identified as correlating with nodal involvement. This approach demonstrates the potential of using exosomal surface markers to establish a blood-based, cost-effective method for early detection of LCa. Exosome Profiling Service provides comprehensive profiling of exosomal surface markers from biofluids, enabling analysis of disease-associated signatures. Suitable for identifying distinct exosome phenotypes, it facilitates studies focused on early detection and disease characterization.

 



Bocchetti, M. et al. J Transl Med. 2024.

Figure 4. Profling of Exosomal Surface Epitopes

 

Deliverables  

1. Particle size distribution, concentration, and marker expression profiles

2. Surface membrane protein and intracellular protein analysis results

3. miRNA sequencing, mRNA/lncRNA targeted panel analysis results

 

Exosomes carry molecular codes that are redefining the boundaries of disease diagnosis and treatment. With professional expertise and efficient service, we help you unlock the vast potential of this microscopic world. Whether for basic research or clinical translation, we offer full-cycle support from experimental design to data interpretation. Contact us now for a tailored analysis plan and advance toward the future of precision medicine!