Exosomics Analysis Service

Exosomes are a class of extracellular vesicles with diameters ranging from 30 to 150 nm. They are actively secreted by various cells and are widely present in body fluids such as blood, urine, and saliva. As key messengers for intercellular communication, exosomes carry a rich “molecular fingerprint” of biomolecules—including proteins, nucleic acids (such as microRNAs and lncRNAs), and lipids—that can accurately reflect the physiological or pathological state of their parent cells. Exosomics Analysis is a high-throughput strategy for systematically deciphering the molecular composition and functions of exosomes. By isolating, purifying, and detecting the molecular characteristics of exosomes, this approach uncovers disease biomarkers or therapeutic targets, providing scientific evidence for studies on disease mechanisms, early diagnosis, and personalized treatment.

 

Services at MtoZ Biolabs

MtoZ Biolabs offers comprehensive exosomics analysis service covering the entire process from sample preprocessing, exosome isolation and purification, and quality validation to molecular characterization (RNA, proteins, and lipids). Through a standardized technological system, we help clients capture high-quality exosomes from complex samples and uncover their potential biomedical value. Our key focuses include:

1. Isolation and High-Purity Preparation of Exosomes

Using a combined strategy of differential ultracentrifugation and size exclusion chromatography, we have established a “standardized + customized” isolation system tailored for various sample types (starting from as little as 0.5 mL of blood, urine, or cell supernatant). This approach ensures exosome purity >95% and maintains exosome integrity and bioactivity throughout the process, effectively addressing interferences from platelet fragments, lipoproteins, etc.

 

2. Accurate Characterization and Quality Verification

• Physical Properties: Nanoparticle tracking analysis (NTA) is used to determine particle size distribution (30–150 nm) and concentration.
• Morphological Verification: Transmission electron microscopy (TEM) confirms the intact vesicular structure.
• Molecular Markers: Western Blot (for markers such as CD63/TSG101) and flow cytometry (FACS) quantitatively assess specific surface proteins.

 

3. Multidimensional Molecular Content Analysis

• Nucleic Acid Analysis: Small RNA/mRNA sequencing is employed to explore the functional profiles of miRNAs and lncRNAs.
• Proteomics Analysis: Utilizing ultra-sensitive mass spectrometry and targeted ELISA, we quantify 20+ clinically relevant biomarkers (e.g., PD-L1, HER2).
• Lipid Metabolism Studies: Integrated mass spectrometry is used to analyze lipid molecules and their association with diseases.

 

4. Functional Validation

Exosome uptake experiments and cellular functional modulation validation (e.g., migration, apoptosis).

 

5. Customized Development

Tailor-made detection schemes based on client-specific marker combinations.

 

Analysis Workflow

1. Sample Submission and Preprocessing

Clients provide samples which are subjected to standardized treatments such as centrifugation to remove debris and the addition of protease inhibitors.

 

2. Exosome Isolation and Purification

The optimal method is selected based on sample characteristics (e.g., combining ultracentrifugation with density gradient purification) to maximize the removal of contaminating proteins and lipids.

 

3. Quality Verification

NTA is used for size distribution analysis (30–150 nm), TEM confirms morphological integrity, and Western Blot validates marker proteins.

 

4. Molecular Characterization

• RNA Analysis: Small RNA-seq is performed to profile miRNA/lncRNA expression and identify differential molecules.
• Protein Analysis: Targeted quantification of 20+ clinical markers is carried out.

 

5. Data Delivery and Interpretation

Original data, visual charts, and personalized analysis recommendations (such as the association of biomarkers with disease) are provided.

 



Figure 1. Workflow for the Proteomic Characterization of Exosomes

 

Why Choose MtoZ Biolabs?

1. High Sensitivity and Specificity

• Detection limits as low as 10 exosomes/μL, suitable for low-abundance samples.
• Proprietary techniques for removing contaminant proteins ensure accurate and reliable results.

 

2. One-Stop Solution

Comprehensive management of the entire process from sample processing to data interpretation, eliminating the need for additional equipment investments.

 

3. Flexible Technological Combinations

Supports both single-target detection and integrated omics analyses, adaptable to different research budgets.

 

4. Strict Quality Control System

Each batch includes built-in positive/negative controls, with traceable quality control data.

 

5. Fast Turnaround and Professional Support

• Typical project cycles range from 10–15 working days (from sample receipt to report delivery).
• Our experienced technical team provides solution design, experimental optimization, and result consultation.

 

Applications

• Early Diagnosis: Non-invasive detection using exosome biomarkers from body fluids, enabling early warnings for tumors, neurological diseases, etc.
• Therapeutic Monitoring: Dynamic tracking of exosomal molecular changes to assess drug efficacy or resistance in real time.
• Mechanism Elucidation: Unraveling the regulatory networks of exosomes in disease progression to accelerate the development of targeted therapies.
• Drug Development: Validating the encapsulation efficiency of exosomes as drug carriers and optimizing delivery system designs.

 

Case Study

1. Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the Highest Abundant Protein and a Putative Universal Biomarker

 



Kugeratski, FG. et al. Nat Cell Biol. 2021.

Figure 2. Super-SILAC-based Proteomics Identifies the Core Proteome of Exosomes

 

2. One-Pot Exosome Proteomics Enabled by a Photocleavable Surfactant

 



Buck, KM. et al. Anal Chem. 2022.

Figure 3. Exosome Proteomics Results Enabled by the Azo Surfactant

 

Exosomics is rapidly emerging as the next growth engine in the life sciences field. At MtoZ Biolabs, we are committed to technological innovation and service quality, helping you unlock the limitless potential of exosomes. Whether you are exploring disease mechanisms or developing novel diagnostic and therapeutic tools, we provide precise data and efficient delivery to support your research and clinical translation. Contact us today to receive a personalized solution!