Cyro-EM Services
Cryogenic electron microscopy (Cryo-EM) is a cutting-edge imaging technique that enables the visualization of three-dimensional structures of biological macromolecules at near-atomic resolution. Based on the rapid cryo-fixation of samples in their native state, Cryo-EM captures thousands of two-dimensional projections using a transmission electron microscope. These images are computationally reconstructed to generate high-resolution 3D structures of proteins, nucleic acid complexes, and other macromolecules. Unlike traditional crystallography, Cryo-EM eliminates the need for crystallization, making it especially suitable for large complexes, flexible assemblies, and molecules that are difficult to crystallize.
Cryo-em has become a core technology in structural biology, playing an essential role in revealing protein conformational changes, deciphering disease mechanisms, and aiding drug design. As a professional CRO for structural analysis, MtoZ Biolabs leverages advanced Cryo-EM platforms and an experienced structural biology team to provide high-quality, end-to-end cryo-em services for researchers and drug developers worldwide. We empower our clients with critical structural insights to accelerate innovative drug discovery and mechanistic research.
Services at MtoZ Biolabs
MtoZ Biolabs offers comprehensive cryo-electron microscopy (Cryo-EM) services, covering core imaging technologies, macromolecular structure characterization, and drug development applications. Our platform supports single particle analysis, cryo-electron tomography, MicroED, and lipid nanodisc assembly, enabling high-resolution analysis of membrane proteins, protein complexes, small proteins, nucleic acid complexes, receptors, viruses, LNPs, and exosomes. We also provide structural insights for gene delivery systems, vaccine development, and drug stability testing. Combined with X-ray crystallography, NMR, and mass spectrometry, our Cryo-EM services deliver integrated solutions for structural biology and pharmaceutical research.
1. Core Technology
2. Macromolecular Structure Characterization
We still offer other sample analysis by cryo-em, please contact us for more detailed information.
3. Drug Development Services
4. Other Service
X-ray crystallography, nuclear magnetic resonance (NMR), and mass spectrometry are indispensable tools in modern structural biology. MtoZ Biolabs also offers services including Epitope Mapping Service, Model Reconstruction of Crystal Structures Using Cryo-EM and NMR, Combinational Applications of Cryo-EM, X-ray Crystallography and NMR, Combinational Applications of Cryo-EM and Mass Spectrometry and related studies, meeting the diverse needs of researchers. We warmly welcome researchers to contact us for more information about our cryo-em services.
Analysis Workflow
Our cryo-em services follows a standardized, traceable workflow to ensure reproducibility and data reliability:
1. Project Consultation and Planning – Assessment of sample characteristics and research goals to design a tailored experimental strategy
2. Sample Preparation and Quality Control – Evaluation of sample concentration, homogeneity, and initial negative-stain screening
3. Grid Preparation and Cryo-Fixation – Preparation of cryo-grids using a Vitrobot or equivalent system followed by rapid plunge-freezing
4. Image Acquisition and Preprocessing – Automated data collection on Cryo-EM platforms; drift correction and particle picking
5. 3D Reconstruction and Structural Modeling – 3D map generation using advanced algorithms; heterogeneity classification and model building
6. Result Delivery and Report – Final delivery includes maps, structural models, and detailed technical documentation
Service Advantages
1. Comprehensive Platform Integration
We are equipped with advanced cryo-electron microscopes and detectors, supported by a robust computational infrastructure. Our platform supports high-throughput sample processing and high-resolution reconstructions, meeting the demands of both academic and industrial projects.
2. Cross-Disciplinary Technical Expertise
We integrate expertise in structural biology, protein expression and purification, sample preparation, and image processing. Dedicated project managers ensure coordinated workflows that reduce trial-and-error costs and shorten turnaround time.
3. Broad Sample Compatibility
Our cryo-em services support a wide variety of samples, including soluble proteins, membrane proteins, protein complexes, viruses, nanoparticles, and flexible or heterogeneous assemblies. Each is addressed with a tailored preparation and data analysis approach.
4. Customized Service Solutions
We tailor services based on research stage (e.g., discovery, target validation, preclinical development). Whether for one-time structural analysis or long-term support, we provide adaptable solutions suitable for publication, patent filing, or regulatory use.
5. Transparent Pricing
Our pricing model is clear and straightforward. One-time-charge with no hidden fees or unexpected costs.
Sample Submission Suggestions
1. High-purity protein or protein complexes (≥ 0.5 mg/mL, in buffer systems without high salt or glycerol)
2. Particulate samples such as viruses, ribosomes, or nanoparticles (good homogeneity, no visible precipitation)
3. Samples should be shipped under cold chain conditions. Please provide detailed information including sample name, concentration, buffer composition, and pH.
If you require sample preparation assistance, please contact us for submission guidelines and custom recommendations.
Deliverables
1. Raw Cryo-EM micrographs and pre-processing data
2. 3D reconstructed density maps (.mrc files)
3. 3D atomic models (.pdb files, where applicable)
4. Comprehensive project report (including workflow, parameters, resolution assessments)
5. Structural interpretation and application guidance (optional)
In the era of precision medicine and structure-driven drug discovery, cryo-em has become an indispensable tool for unraveling molecular mechanisms and advancing therapeutic development. At MtoZ Biolabs, our advanced infrastructure, rigorous workflows, and expert technical team are dedicated to delivering high-resolution structural insights through reliable cryo-em services.
Whether you're looking to resolve critical protein structures, optimize molecular designs, or validate mechanism-of-action, we invite you to contact us for tailored service plans and expert support.
Related Services
Cryo-EM De Novo Structure Analysis Service
Cryo-EM Feasibility Assessment Service
Cryo-Electron Microscopy Based Service
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