Cryo-EM Feasibility Assessment Service
- Imaging quality analysis
- Ice layer evaluation
- Particle density and consistency assessment
- 2D class averages and representative micrographs
- Preliminary 3D reconstruction (if applicable)
- Sample optimization advice and feasibility conclusion
- Sample screening and optimization for single-particle analysis (SPA)
- Feasibility evaluation for membrane proteins, viruses, antibody–antigen complexes
- Pre-screening for Cryo-Electron Tomography (Cryo-ET) projects
- Preparation for studying complex multi-protein assemblies
- Investigating binding mechanisms and conformational dynamics for drug design
Cryo-electron microscopy (Cryo-EM) feasibility assessment is a critical preliminary step conducted prior to initiating high-resolution 3D reconstruction projects. It involves a systematic evaluation of sample imaging quality, particle distribution, ice thickness, and data acquisition potential. By implementing a scientific and standardized assessment approach, potential issues can be identified early, allowing for precise optimization strategies that significantly increase the success rate and data quality of subsequent Cryo-EM studies.
With the rapid advancement of Cryo-EM in terms of resolution and scope, Cryo-EM feasibility assessment has become indispensable in drug discovery, structural biology, vaccine development, and beyond. An effective feasibility assessment saves resources, shortens timelines, and accelerates scientific discovery. MtoZ Biolabs, equipped with advanced instrumentation and extensive sample processing experience, delivers comprehensive and accurate Cryo-EM Feasibility Assessment Service to efficiently support your research projects.
Services at MtoZ Biolabs
MtoZ Biolabs conducts the Cryo-EM Feasibility Assessment Service through a standardized yet flexible workflow that includes the following key steps:
1. Negative Staining TEM Screening
Rapid evaluation of sample integrity, aggregation, and dispersion using negative-stain transmission electron microscopy to guide subsequent vitrification decisions.
2. Cryo-Grid Preparation and Optimization
Tailored grid preparation under various vitrification conditions to optimize ice thickness, particle distribution, and orientation based on sample properties.
3. Cryo-EM Data Acquisition
High-throughput micrograph collection on advanced Cryo-EM platforms to comprehensively characterize sample behavior.
4. 2D Classification and Preliminary Modeling
Automated particle picking and 2D classification to assess particle consistency; preliminary 3D models may be generated when data quality permits.
5. Comprehensive Assessment Report
Delivery of a detailed report including:
Through professional and standardized Cryo-EM Feasibility Assessment Service, MtoZ Biolabs supports the efficient launch of your structural biology projects.
Why Choose MtoZ Biolabs?
✅ Advanced Instrumentation: High-end Cryo-EM systems and direct electron detectors ensure superior micrograph quality and reliable data.
✅ Experienced Technical Team: Extensive expertise in sample handling, grid optimization, and data analysis for various challenging specimens.
✅ Fast Turnaround: Standardized workflows allow assessment and report delivery within one week.
✅ Customized Follow-up Support: Optimization strategies and high-resolution acquisition plans tailored to assessment outcomes ensure seamless project progression.
✅ One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Applications
The Cryo-EM Feasibility Assessment Service supports diverse structural biology and drug development applications, including but not limited to:
FAQ
Q1: Why is negative staining TEM screening necessary before vitrification?
Although resolution is limited, negative staining provides a fast, low-cost method to assess sample aggregation, degradation, and dispersion. This step helps identify problematic samples before committing to more expensive Cryo-EM procedures. MtoZ Biolabs includes this step in its Cryo-EM Feasibility Assessment Service to ensure rigorous quality control.
Q2: Can a preliminary 3D model be generated during feasibility assessment?
Yes. If micrograph quality and particle orientation are sufficient, MtoZ Biolabs can generate an initial 3D reconstruction to provide insights into structural features and potential resolution limits, supporting informed decision-making.
Q3: Is the Cryo-EM Feasibility Assessment Service suitable for small, dynamic samples?
Yes, though they are more challenging. MtoZ Biolabs has extensive experience with small, flexible molecules such as ion channels and GPCRs. By employing advanced optimization strategies and acquisition techniques, we ensure improved imaging outcomes and a solid foundation for structural analysis.
MtoZ Biolabs' Cryo-EM Feasibility Assessment Service provides the technical depth and operational efficiency required to launch high-quality Cryo-EM projects with confidence. Contact us today to learn how we can help ensure your structural biology study begins on the right foot.
How to order?
