Subcellular Organelles Structure Characterization Service | Cryo-EM

Subcellular Organelles Structure Characterization Service based on Cryo-EM focuses on the high-resolution analysis of various subcellular organelles under near-native conditions. Utilizing cryo-electron microscopy (Cryo-EM) and cryo-electron tomography (Cryo-ET), Subcellular Organelles Structure Characterization Service enables the direct visualization of the spatial organization, internal architecture, and functional details of organelles. It provides critical insights into fundamental cellular mechanisms, disease progression pathways, and biomedical applications.

 

Driven by advances in Cryo-EM technologies, the structural elucidation of organelles has reached unprecedented resolution levels. Cryo-ET, as a powerful extension of Cryo-EM, reconstructs the three-dimensional ultrastructure of complex intracellular environments by combining multiple tilt-series images. It is particularly suited for studying organelles such as mitochondria, endoplasmic reticulum, cytoskeleton, and nuclear pore complexes under in situ, non-destructive conditions.

 



Figure 1. Schematic Overview of Major Subcellular Organelles

 

MtoZ Biolabs offers specialized Subcellular Organelles Structure Characterization Service supported by advanced Cryo-EM platform, state-of-the-art direct electron detectors and professional data processing pipelines. This service empowers researchers to accelerate life science investigations and translational medicine development.

 

Services at MtoZ Biolabs

Within the Subcellular Organelles Structure Characterization Service based on Cryo-EM, MtoZ Biolabs provides an integrated workflow covering sample preparation, imaging, data processing, and 3D reconstruction.

We offer high-quality structural characterization for:

• Mitochondria
• Golgi apparatus
• Lysosomes
• Endoplasmic reticulum
• Autophagosomes
• Nuclear pore complexes
• Exosomes and microvesicles
• Other specific subcellular organelles

 

Why Choose MtoZ Biolabs?

The Subcellular Organelles Structure Characterization Service based on Cryo-EM offers a series of key advantages:

✅ True in situ analysis: Direct observation of organelles in their native environment without excessive processing.

✅ Broad spatial scale: Suitable for organelles ranging from hundreds of nanometers to several micrometers.

✅ Versatile sample compatibility: Applicable to fresh tissues, primary cells, established cell lines, and tissue sections.

✅ Extensive experience: Proven expertise across diverse organelles including mitochondria, ER, autophagosomes, and nuclear pores.

✅ Tailored analysis solutions: Options for full 3D reconstructions, dynamic state evaluations, and localized fine-structure modeling according to project needs.

 

Applications

The Subcellular Organelles Structure Characterization Service based on Cryo-EM is widely applicable across both fundamental research and applied biomedical fields:

· Ultrastructural change detection: Investigating mitochondrial fragmentation, autophagosome formation, and Golgi disassembly under pathological conditions.

· Mechanistic studies: Elucidating interactions between organelles, transport pathways, and dynamic reorganizations.

· Biomarker discovery: Identifying potential early diagnostic markers or therapeutic targets based on organelle structural features.

· Drug development support: Assessing the effects of drug candidates on mitochondria, lysosomes, and other critical organelles.

· Synthetic biology and nanobiotechnology: Engineering biomimetic structures and developing functional nanomaterials.

 

Case Study

Case 1: Cryo-EM Unveils PML Nuclear Bodies Assembly Mechanism

In a recent study, researchers utilized Cryo-EM, cross-linking mass spectrometry (XL-MS), and AlphaFold2 structural predictions to resolve the assembly mechanism of PML nuclear bodies (PML-NBs). They discovered that PML dimers form large membraneless structures through α6 helix-mediated reverse cross-linking in an “octopus-like” configuration. This breakthrough provided crucial insights into the roles of PML in tumor suppression and antiviral responses and offered structural guidance for new therapies against acute promyelocytic leukemia.

 



Tan, Y. et al. Cell Discov. 2024.

 

This study highlights the unique advantages of Cryo-EM in elucidating the assembly mechanisms of subcellular organelles. MtoZ Biolabs offers a Subcellular Organelles Structure Characterization Service based on Cryo-EM to help researchers explore the intricate relationship between organelle structure and function.

 

FAQ

Q1: What are the critical requirements for sample preparation in Subcellular Organelles Structure Characterization Service based on Cryo-EM?

High structural integrity, minimal background contamination, and rapid vitrification are essential. MtoZ Biolabs offers tailored protocols for different organelles to ensure optimal preservation of native ultrastructure.

 

Q2: What advantages does Cryo-ET offer in studying organelle structures?

Cryo-ET allows in situ, three-dimensional visualization of organelles and their internal components without crystallization. It is particularly suited for studying highly heterogeneous and spatially complex cellular environments.

 

Q3: Is it necessary to purify organelles before analysis? Can whole-cell samples be directly imaged?

Both approaches are viable. Purified organelle samples yield higher resolution and contrast, while whole-cell imaging provides insights into the spatial organization and dynamics of organelles within the physiological cellular context.

 

By leveraging the Subcellular Organelles Structure Characterization Service based on Cryo-EM, MtoZ Biolabs is committed to advancing research into intracellular architectures, supporting life science innovations and driving new discoveries in translational medicine.


Contact us to learn more or request a customized project plan!