Mass Spectrometry-Based Quantitative Proteomics Service
- Sample Preparation: Receive cell, tissue, or biofluid samples, followed by lysis, protein extraction, and enzymatic digestion according to the experimental design.
- Separation and Labeling: Choose from label-free, chemical labeling, or isotope labeling strategies based on project requirements.
- Mass Spectrometry Detection: Perform analysis using Orbitrap Fusion Lumos, Q Exactive HF, Q-TOF, or MALDI-TOF platforms.
- Data Acquisition and Processing: Extract peaks, perform database searches, and conduct quantitative analysis using professional software.
- Result Delivery: Generate comprehensive protein quantification reports and analysis results.
- Sample Types: Cells, tissues, serum/plasma, urine, cerebrospinal fluid, etc.
- Storage and Transport: Samples should be stored at -80°C and shipped on dry ice.
- Information Details: Please provide sample concentration, volume, source, processing method, and experimental design information.
- Special Considerations: If the sample quantity is limited or contains interfering substances, please notify us in advance so we can provide optimized solutions.
- Experimental Report: Includes protein quantification lists, differential analysis results, and database search information.
- Data Files: Raw mass spectrometry files and processed data files.
- Analytical Support: Basic statistics, data visualization, and technical consultation support.
Quantitative protein analysis is one of the core aspects of modern life science and biopharmaceutical research. By comparing differences in protein abundance across different samples or treatment conditions, researchers can gain deeper insights into cellular signaling pathways, disease mechanisms, and potential biomarkers. Traditional protein detection methods, such as Western blot or ELISA, although highly specific at the single-protein level, have clear limitations when analyzing large-scale protein groups simultaneously.
The rapid advancement of mass spectrometry has brought breakthroughs to proteomics research. Mass spectrometry-based quantitative proteomics integrates high-resolution mass spectrometry with advanced sample separation strategies, enabling the quantification of thousands of proteins in a single experiment. It supports both relative and absolute quantification. With extensive experience in mass spectrometry platforms and standardized workflows, MtoZ Biolabs provides a reliable mass spectrometry-based quantitative proteomics service to researchers worldwide, facilitating both fundamental and applied research.
Services at MtoZ Biolabs
MtoZ Biolabs’ mass spectrometry-based quantitative proteomics service covers a wide range of technical approaches to meet diverse research needs.
Analysis Workflow
Service Advantages
1. Multi-Platform Technical Integration
MtoZ Biolabs is equipped with multiple mass spectrometry instruments, including Orbitrap Fusion Lumos, Q Exactive HF, Q-TOF, and MALDI-TOF. These instruments complement each other, ensuring both broad coverage and high sensitivity, delivering results with optimal resolution, speed, and depth.
2. Diverse Quantification Strategies
We provide multiple quantitative approaches, including label-free, labeled, targeted, and untargeted methods. Researchers can choose the most suitable strategy based on their project scale, budget, and objectives.
3. Expertise in PTM Quantification
For post-translational modifications (PTMs) such as phosphorylation, acetylation, ubiquitination, and glycosylation, we offer specialized enrichment and quantitative analysis services. Our standardized workflows, combined with mass spectrometry, enable in-depth exploration of protein modifications in cellular regulation.
4. Standardization and Quality Control
From sample preparation to mass spectrometry acquisition and data processing, our mass spectrometry-based quantitative proteomics service follows strict SOPs and QC standards to ensure stability and reproducibility. All processes are performed under controlled conditions to guarantee data reliability.
5. One-Time Charge
Our pricing is transparent, with no hidden fees or additional costs.
FAQs
Q1: How long does the mass spectrometry-based quantitative proteomics service take to deliver results?
A: Typically within 2–4 weeks, depending on sample quantity and analysis complexity.
Sample Submission Suggestions
Deliverables
MtoZ Biolabs is dedicated to providing a high-quality, standardized mass spectrometry-based quantitative proteomics service. Whether for exploratory studies or validation experiments, we deliver reliable data support and tailored solutions. Contact us today to make MtoZ Biolabs your trusted partner in advancing proteomics research.
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