Particular Post-Translational Modifications Quantitative Analysis Service
Proteins are the central executors of life activities, and their functions are determined not only by amino acid sequences but also by post-translational modifications (PTMs). PTMs refer to structural and functional changes in proteins caused by chemical modifications after translation, including phosphorylation, acetylation, methylation, ubiquitination, glycosylation, acylation, lactylation, and many others. These modifications are widely involved in signal transduction, metabolic regulation, gene expression, protein stability, and degradation.
In recent years, with the rapid development of mass spectrometry and high-throughput technologies, quantitative studies of PTMs have become crucial for elucidating biological mechanisms, exploring disease-related processes, and advancing drug development. Quantitative PTM analysis not only reveals dynamic changes at modification sites but also helps researchers understand the molecular basis of protein functional regulation.
As a CRO specializing in proteomics research, MtoZ Biolabs is committed to providing high-quality mass spectrometry services for global academic and pharmaceutical clients. Using multiple quantitative strategies, including SILAC, iTRAQ/TMT, Label-Free, DIA/SWATH, and MRM/PRM, we offer a particular post-translational modifications quantitative analysis service that covers a broad spectrum of PTMs such as phosphorylation, acetylation, methylation, ubiquitination, glycosylation, acylation, and lactylation, enabling researchers to comprehensively analyze modification networks and accelerate both basic and applied studies.
Services at MtoZ Biolabs
MtoZ Biolabs’ particular post-translational modifications quantitative analysis service is designed to meet the needs of both academic and industrial clients in PTM quantification research. Our service includes, but is not limited to:
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 Lysine 2-hydroxyisobutyrylation Quantitative Analysis Service |
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In addition to the PTM types listed above, we are also able to perform quantitative analysis of other post-translational modifications. If you have requirements for PTM types not mentioned here, please contact our staff.
At MtoZ Biolabs, the particular post-translational modifications quantitative analysis service is carried out using multiple complementary strategies, including SILAC-based metabolic labeling, iTRAQ/TMT isobaric labeling, label-free quantification, DIA/SWATH data-independent acquisition, and targeted validation with MRM/PRM. By integrating high-resolution LC-MS/MS platforms with optimized enrichment techniques, these methods enable sensitive, accurate, and reproducible analysis of diverse post-translational modifications across various biological samples.
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Analysis Workflow
1. Sample preparation: Protein, cell, or tissue samples are extracted and digested.
2. Modification enrichment: Specific enrichment methods (IMAC, antibody-based, etc.) are applied for different PTMs.
3. Mass spectrometry detection: High-resolution LC-MS/MS platforms are used for detection.
4. Data analysis: Database searching and algorithms identify and quantify modification sites.
5. Reporting: Comprehensive results and data files are delivered to clients.
Service Advantages
1. Wide PTM coverage
Our particular post-translational modifications quantitative analysis service covers a broad spectrum of PTMs, ranging from classical phosphorylation and acetylation to emerging modifications such as lactylation and glutarylation. This ensures effective support across diverse research areas.
2. Multi-platform quantification
By combining SILAC, iTRAQ/TMT, Label-Free, DIA/SWATH, and MRM/PRM strategies, we select the most suitable approach for each project, balancing sensitivity, coverage, and reproducibility.
3. Customized service
MtoZ Biolabs provides tailored PTM quantification analysis. We can perform targeted studies for specific modifications such as phosphorylation, acetylation, methylation, ubiquitination, or glycosylation, and also offer combined PTM detection. Experimental strategies are designed based on sample properties and research objectives, ensuring reliable and research-oriented data.
4. Experienced technical team
Our team has extensive PTM research experience and offers professional consultation to optimize study design, improving efficiency and data reliability.
5. One-Time-Charge
Our pricing is transparent, with no hidden fees or unexpected costs.
Sample Submission Suggestions
1. Sample types: Protein, cell, tissue, serum, plasma, etc.
2. Sample purity: Protein purity >80% is recommended.
3. Storage: Samples should be frozen at low temperature and avoid repeated freeze–thaw cycles.
4. Additional notes: Please provide sample origin, preparation details, and research goals to optimize the experimental strategy.
Deliverables
1. Protein and Modification Site Identification List
2. Quantitative Analysis Results (Differentially Modified Sites)
3. Statistical and Visualization Data
4. Raw Mass Spectrometry Files
5. Comprehensive Analysis Report
Quantitative analysis of post-translational modifications is essential for understanding protein functional regulation and biological processes. With advanced mass spectrometry platforms, validated quantitative strategies, and a skilled technical team, MtoZ Biolabs provides reliable particular post-translational modifications quantitative analysis service. If you are seeking high-quality PTM quantification solutions, please contact us. We are dedicated to supporting your research and development needs.
Related Services
PTM Mass Spectrometry Analysis Service
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