Quantitative Methylomics Service

    Methylation represents one of the most prevalent post-translational modifications, chiefly affecting transcription factors and histones, though it also modifies a subset of cytoplasmic proteins. This modification includes monomethylation, symmetric/asymmetric dimethylation, and trimethylation on arginine (R) and lysine (K) residues. Arginine methylation plays critical roles in RNA processing, gene transcription, DNA damage repair, protein translocation, and signal transduction, whereas lysine methylation primarily influences histone function and epigenetic regulation of gene transcription. Targeted enrichment of methylated peptides using modification-specific antibodies, followed by LC-MS/MS analysis, facilitates extensive quantitative and qualitative assessment of protein methylation.

     

    MtoZ Biolabs employs the Orbitrap Fusion Lumos mass spectrometer and nanoLC from Thermo Fisher for comprehensive protein methylation identification services. Clients need only submit their experimental aims and samples; MtoZ Biolabs handles all subsequent steps, including protein extraction, digestion, methylation specific peptide enrichment, peptide separation, mass spectrometric analysis, analysis of raw data, and bioinformatics.

     

    Analysis Workflow

     

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    Sample Submission Requirements

    • Plant Tissues (roots, xylem, phloem): > 5 g
    • Animal Tissues: > 200 mg wet weight
    • Microorganisms: > 2 g wet weight
    • Body Fluids (saliva, amniotic fluid, CSF): > 10 mL
    • Serum: > 500 µL
    • Urine: > 50 µL
    • Protein Extracts: > 2 mg/mL concentration, minimum total 1 mg, free of interfering substances like nucleic acids, lipids, and polysaccharides.

    1. For Tissue Samples 

    Ship on dry ice.

     

    2. For Protein Samples 

    Use standard lysis solutions for tissue and cell extraction.

     

    3. Sample Shipping

    Transport samples with adequate dry ice and opt for expedited shipping to minimize degradation risks during transit.

     

    4. Sample Testing

    We evaluate all samples before commencing the actual experiments. Testing must confirm sample integrity before proceeding with formal analyses.

     

    Our team, seasoned in protocol optimization, ensures top-quality results in protein methylation studies. For specific inquiries, please contact us directly.

     

    Service Advantages

    1. High-Throughput

    Capability to identify and quantify thousands of proteins simultaneously.

     

    2. Complete Coverage

    Digestion with 2-3 enzymes guarantees comprehensive protein analysis.

     

    3. Applicability to Low-Abundance Methylated Proteins

     

    4. State-of-the-Art Equipment

    The Orbitrap Fusion Lumos, noted for its superior resolution and sensitivity.

     

    Services at MtoZ Biolabs

    1. Ion Chromatography

    2. High Performance Liquid Chromatography (HPLC)

    3. Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS)

     

    Deliverables

    In the technical report, MtoZ Biolabs will provide you with detailed technical information, including: 

    1. Experimental Procedures

    2. Relevant Mass Spectrometry Parameters

    3. Detailed Information on Identified Methylation Sites

    4. Mass Spectrometry Images

    5. Raw Data

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