Protein Trimethylation Analysis
Post-translational modifications (PTMs) refer to the chemical alterations that occur to proteins either during or after translation. PTMs are essential in regulating numerous cellular processes, including cell differentiation, protein degradation, signal transduction, gene expression regulation, and protein-protein interactions. Protein trimethylation, a key post-translational modification, involves the covalent attachment of three methyl groups (-CH3) to specific residues, typically lysine or arginine. Trimethylation plays critical biological roles, particularly in gene regulation, epigenetic modulation, and signal transduction pathways. Comprehensive analysis of protein trimethylation (target protein trimethylation analysis and trimethylation proteomics) not only enhances our understanding of fundamental cellular processes but also identifies promising therapeutic targets for diseases such as cancer.
Services at MtoZ Biolabs
1. Target protein trimethylation analysis
MtoZ Biolabs provides Target Protein Trimethylation Analysis designed to detect and characterize trimethylation modifications on specific proteins of interest. By combining immunoprecipitation (Co-IP) with high-resolution LC–MS/MS, this service enables precise identification of trimethylated lysine or arginine residues and accurate quantification of modification levels. Using modification-specific antibodies (anti-Kme3 or anti-Rme3), we selectively enrich methylated proteins or peptides, ensuring high detection sensitivity. This targeted approach supports detailed mechanistic studies and functional validation of protein post-translational methylation events.
2. Trimethylation proteomics analysis
MtoZ Biolabs' Trimethylation Proteomics Analysis offers a global and systematic solution to profile protein trimethylation across biological samples. Utilizing high-sensitivity mass spectrometry combined with pan–Kme3 and pan–Rme3 antibody–based enrichment, this service enables large-scale identification and quantification of trimethylated peptides and proteins. Through optimized sample preparation and bioinformatics-driven analysis, we reveal modification landscapes, site distributions, and functional pathways associated with methylation regulation. This comprehensive analysis provides valuable insights into epigenetic regulation, signaling dynamics, and protein interaction networks.
Analysis Workflow
Service Advantages
1. Advance Analysis Platform: Mtoz Biolabs established an advanced protein trimethylation analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time Charge: Our pricing is transparent, with no hidden fees or additional costs.
3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all protein trimethylation analysis data providing clients with acomprehensive data report.
4. Comprehensive Analysis: Both target protein trimethylation analysis and trimethylation proteomics can be conducted in MtoZ Biolabs.
Sample Submission Requirements
1. If you are providing tissue samples, please ship them to us on dry ice.
2. If you are providing protein samples, standard tissue or cell lysis buffers can be used during protein extraction.
3. Sample transportation: Please ensure that samples are shipped with sufficient dry ice and choose the fastest possible shipping way to minimize the risk of sample degradation during transit.
4. Before proceeding with the formal experiment, we will test the samples you provide, and the formal analysis will begin once the samples pass the quality check.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. The Detailed Information of Protein Trimethylation Analysis
4. Mass Spectrometry Image
5. Bioinformatics Analysis
6. Raw Data
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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