Protein Monomethylation Analysis
Protein methylation, a common post-translational modification (PTM), plays a pivotal role in regulating biological processes such as gene expression, signal transduction, and chromatin organization. Based on the level of methyl group addition, protein methylation occurs in three forms: monomethylation, dimethylation, and trimethylation. Monomethylation, specifically, involves the addition of one methyl group to lysine (Lys) or arginine (Arg) residues, facilitating precise regulation of processes such as gene control, chromatin state modulation, and signal transduction. Compared to dimethylation and trimethylation, monomethylation exhibits greater versatility in modulating various cellular functions.
Figure 1. Lysine Monomethylation
MtoZ Biolabs offers comprehensive Protein Monomethylation Analysis services utilizing high-performance liquid chromatography-mass spectrometry (HPLC-MS). This advanced technique integrates the separation capabilities of HPLC with the high sensitivity and specificity of MS, enabling accurate identification and quantification of monomethylation sites. Our service assists researchers in gaining a deeper understanding of monomethylation's roles in cellular metabolism, gene regulation, and disease mechanisms. Covering the entire analytical workflow, from sample preparation to data interpretation, we ensure high-quality experimental results and actionable insights for our clients.
Analysis Workflow
Services at MtoZ Biolabs
1. Targeted Protein Monomethylation Analysis
MtoZ Biolabs offers Targeted Protein Monomethylation Analysis designed to characterize specific methylated proteins provided by the client, such as histones H3 and H4 or other regulatory proteins of interest. Through high-resolution LC-MS/MS combined with immunoprecipitation (IP)-based enrichment, we accurately identify and quantify monomethylation sites on target proteins. This targeted approach allows researchers to elucidate how protein-specific monomethylation modulates gene expression, chromatin dynamics, and cellular signaling. The service is ideally suited for in-depth studies in fields such as epigenetics, oncology, and metabolic regulation.
2. Monomethylation Proteomics
Our monomethylation proteomics service offers a comprehensive analysis of monomethylation across the entire proteome. Leveraging a high-throughput mass spectrometry platform, we deliver a panoramic view of methylation modifications across various samples, uncovering extensive methylation patterns. This service is extensively used in epigenetic studies, cancer research, and metabolomics, aiding researchers in identifying potential biomarkers and regulatory networks, ultimately providing a complete protein modification map to better understand disease mechanisms.
Service Advantages
1. Advance Analysis Platform
MtoZ Biolabs established an advanced protein PTM analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. High-Data-Quality
Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate PTM proteomics analysis data providing clients with a comprehensive data report.
3. Comprehensive Coverage
Using both TMT/iTRAQ labeling and Label-Free techniques for sample analysis significantly enhances experimental efficiency while ensuring high-quality data.
4. One-Time-Charge
Our pricing is transparent, no hidden fees or additional costs.
Sample Submission Requirements
For more sample details, please consult our technical team.
Applications
1. Disease Mechanism Research: Investigating monomethylation's role in complex diseases such as cancer and neurodegenerative disorders, helping to identify biomarkers and potential therapeutic targets.
2. Gene Expression Regulation: Exploring the influence of protein monomethylation on chromatin architecture and gene expression activity.
3. Signal Transduction: Elucidating the regulatory effects of monomethylation in cellular signaling pathways, shedding light on key molecular communication processes.
4. Drug Development: Supporting drug discovery and optimization by targeting specific monomethylation modifications.
Application Case
Characterization of Lysine Monomethylome and Methyltransferase in Model Cyanobacterium Synechocystis sp. PCC 6803
Lin, X. H. et al. Genomics Proteomics Bioinformatics, 2020.
With our cutting-edge mass spectrometry platform and extensive expertise, MtoZ Biolabs offers not only precise monomethylation analysis but also services for a range of methylation modifications (monomethylation, dimethylation, trimethylation) and other PTMs, including propionylation, acetylation, and phosphorylation. We are committed to providing comprehensive technical support to advance your research. Please contact us for further information.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
Related Services
Protein Trimethylation Analysis
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