Protein Lysine Methylation Analysis
- Investigating disease mechanisms
- Screening and validating drug targets
- Discovering biomarkers
- Conducting epigenetic studies
- Analyzing signal transduction pathways
Protein lysine methylation is a crucial post-translational modification (PTM) catalyzed by lysine methyltransferases (KMTs), which add one or more methyl groups to lysine residues on proteins. This modification exists in three forms: monomethylation (Kme1), dimethylation (Kme2), and trimethylation (Kme3). Lysine methylation plays a vital role in regulating chromatin structure, gene expression, signal transduction, and the cell cycle. Aberrations in lysine methylation are associated with various diseases, including cancer, neurodegenerative disorders, and metabolic imbalances.
Figure 1. Lysine Methylation
MtoZ Biolabs utilizes advanced liquid chromatography-mass spectrometry (LC-MS/MS) platforms to offer highly sensitive and precise lysine methylation analysis. Our skilled team and state-of-the-art technology empower researchers to explore the biological significance and underlying mechanisms of lysine methylation in various cellular processes.
Analysis Workflow
Services at MtoZ Biolabs
1. Targeted Protein Lysine Methylation Analysis
This service focuses on the in-depth analysis of lysine methylation on specific proteins designated by clients. By combining immunoaffinity enrichment with LC-MS/MS detection, MtoZ Biolabs can accurately identify mono-, di-, and tri-methylation sites on the target protein, quantify modification levels, and reveal dynamic regulatory patterns. This approach is ideal for researchers aiming to validate modification events, study site-specific functions, or explore the regulatory roles of lysine methylation in signaling and transcriptional control.
2. Lysine Methylation Proteomics
For a comprehensive understanding of lysine methylation across the entire proteome, MtoZ Biolabs provides high-throughput proteomics analysis capable of profiling thousands of methylation sites within complex biological samples. Utilizing methyl-lysine–specific antibodies or pan-methylation enrichment combined with data-dependent or data-independent LC-MS/MS acquisition, this workflow enables global mapping and quantitative comparison of methylation states under different biological conditions. The service supports discovery-driven studies such as pathway mapping, biomarker identification, and epigenetic regulation analysis.
Why Choose MtoZ Biolabs?
1. Advance Analysis Platform: MtoZ Biolabs established an advanced protein PTM analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate PTM proteomics analysis data providing clients with a comprehensive data report.
4. Multi-Sample Compatibility: Our platform supports a wide variety of biological samples, including cells, tissues, blood, and bodily fluids, providing flexibility to accommodate diverse research demands.
5. Customized Service: Tailored to the specific research needs of our clients, we offer flexible experimental design and personalized data analysis to ensure the achievement of research goals to the fullest extent.
Sample Submission Requirements
For more sample details, please consult our technical team.
Applications
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs is dedicated to providing researchers with high-quality, reliable protein PTM analysis services. Our Lysine Methylation Analysis, powered by cutting-edge technology and deep expertise, offers the critical data needed to advance your research. Contact us today to discuss how we can support your scientific goals.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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