Quantitative Glutarylation Service
- Elucidating the role of glutarylation in fatty acid metabolism, mitochondrial function, and energy regulation.
- Examining its effects on nucleosome assembly, chromatin organization, and gene expression control.
- Investigating the regulation of tumor cell proliferation, migration, and apoptosis through glutarylation.
- Exploring the role of glutarylation in neurodegenerative diseases such as Alzheimer's disease.
- Studying its effects on sperm motility and the regulation of reproductive cell function.
Protein glutarylation is a post-translational modification (PTM) where glutaryl groups, such as those derived from glutaryl-CoA, are covalently attached to lysine residues on proteins through enzymatic catalysis. Lysine residues typically carry a positive charge, but glutarylation converts this to a negative charge, altering the charge state of the protein. This change can affect the protein's conformation and interactions with other molecules. Glutarylation is a reversible modification, playing a significant role in nucleosome assembly, chromatin structure regulation, gene expression, DNA damage repair, and cell cycle progression. Moreover, similar to other acylation modifications like acetylation, glutarylation is involved in key metabolic pathways, such as fatty acid synthesis, mitochondrial function, and cellular respiration.
Tan, M. J. et al. Cell Metab. 2014.
Figure 1. Lysine Deglutarylation and Glutaryl-CoA Pathways
Recent studies have underscored the importance of glutarylation in various biological and pathological contexts, including cellular metabolism, cancer progression, neurodegenerative diseases, and reproductive health. Understanding the mechanisms and functions of glutarylation is essential for clarifying its role in both biological and disease-related processes. MtoZ Biolabs offers a comprehensive Quantitative Glutarylation Service based on high-resolution mass spectrometry, enabling accurate detection and quantification of glutarylation modifications on proteins, providing robust support for cutting-edge research.
Analysis Workflow
MtoZ Biolabs' Quantitative Glutarylation Service utilizes high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) in conjunction with specific antibody enrichment methods to precisely identify and quantify glutarylation modification sites on lysine residues in proteins. We use TMT (Tandem Mass Tag) and iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) labeling technologies, alongside label-free approaches, to ensure high-throughput analysis of multiple samples. Our service encompasses every stage from sample preparation and glutarylated peptide enrichment to mass spectrometry analysis and bioinformatics interpretation, ensuring high sensitivity and accuracy to help researchers investigate the functional roles of glutarylation under different biological conditions.
Service Advantages
1. Advance Analysis Platform
MtoZ Biolabs established an advanced protein PTM analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. Comprehensive Coverage
Using both TMT/iTRAQ labeling and Label-Free techniques for sample analysis significantly enhances experimental efficiency while ensuring high-quality data.
3. Quantitative Accuracy
Our cutting-edge quantification methods ensure precise and reliable data, even under varying experimental conditions.
4. One-Time-Charge
Our pricing is transparent, no hidden fees or additional costs.
Sample Submission Requirements
For more sample details, please consult our technical team.
Applications
Quantitative glutarylation analysis has diverse applications across several fields:
Deliverables
What Could be Included in the Report?
1. Comprehensive Experimental details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs is dedicated to advancing your research and clinical projects by providing detailed insights into protein glutarylation's extensive physiological roles through top-tier analytical techniques and expert services. Free project evaluation, welcome to learn more details! Our technical specialists are available to provide a free business assessment.
How to order?
