Quantitative Acetylomics Service

Acylation is a post-translational modification involving the attachment of acyl groups, such as acyl-CoA, to proteins. This modification plays a pivotal role in regulating key biological processes including epigenetics, energy metabolism, protein trafficking, and molecular interactions. Acylation research is a dynamic field in life sciences, with studies expanding beyond acetylation to include propionylation, malonylation, glutarylation, succinylation, and crotonylation. Quantitative proteomics of acylation provides insights into the vital link between protein acylation changes and biological functions, offering valuable information for uncovering life mechanisms, identifying clinical disease markers, and targeting drug discovery.

 

The acylation quantitative proteomics process begins by enzymatically digesting protein samples into peptides. These peptides are then separated using liquid chromatography to simplify the mixture. Specific acylation modifications are enriched using targeted antibodies or kits, followed by quantification with LC-MS/MS.

 

MtoZ Biolabs employs the Thermo Fisher’s Orbitrap Fusion Lumos mass spectrometry system alongside nanoLC platform for comprehensive acylation quantitative proteomics services. We invite researchers to share their experimental goals and submit their samples, after which our team manages all aspects of the analysis. This includes protein extraction, digestion, peptide enrichment, chromatographic separation, mass spectrometric analysis, and subsequent bioinformatics evaluations.

 



Figure 1. Acylation Quantitative Proteomics Analysis

 

Sample Submission Requirements

1. For Tissue Samples 

Ship on dry ice.

 

2. For Protein Samples 

Use standard lysis solutions for tissue and cell extraction.

 

3. Sample Shipping

Transport samples with adequate dry ice and opt for expedited shipping to minimize degradation risks during transit.

 

4. Sample Testing

We evaluate all samples before commencing the actual experiments. Testing must confirm sample integrity before proceeding with formal analyses.

 

Deliverables

In the technical report, MtoZ Biolabs will provide you with detailed technical information, including: 

1. Experimental Procedures

2. Relevant Spectrometric Parameters

3. Comprehensive Details on Identified Acylation Sites

4. Mass Spectrometry Images

5. Raw Data