Succinylation Analysis Service

Succinylation, a dynamic post-translational modification (PTM), involves the covalent attachment of a succinyl group (-CO-CH₂-CH₂-COO-) to lysine residues, altering protein charge, conformation, and functionality. This modification critically regulates metabolic pathways, epigenetic processes, and cellular signaling and connects metabolic intermediates (e.g., succinyl-CoA) to disease mechanisms. Dysregulated succinylation has been implicated in cancer progression, immune disorders, and neurodegenerative diseases (e.g., Alzheimer’s disease). Emerging therapeutic strategies targeting succinylation regulators (e.g., SIRT5/SIRT7 inhibitors) or restoring succinylation homeostasis highlight its clinical relevance. MtoZ Biolabs offers advanced succinylation analysis service to elucidate molecular mechanisms of this PTM, enabling the discovery of biomarkers, therapeutic targets, and disease pathways for precision medicine.

 



Liu, Z. et al. Biomed Pharmacother. 2023.

Figure 1. The Process and Functions of Lysine Succinylation

 

Services at MtoZ Biolabs

Our succinylation analysis service integrates cutting-edge mass spectrometry, bioinformatics pipelines, and PTM expertise to deliver:

1. Global Succinylation Profiling: High-throughput identification and quantitation of succinylation sites across proteomes.

2. Targeted Analysis: In-depth characterization of specific proteins or pathways (e.g., metabolic enzymes, histones).

3. Functional Validation: Multi-omics integration to explore roles of succinylation in protein stability, enzymatic activity, and disease pathology.

 



Figure 2. The Workflow of Succinylation Analysis

 

Service Advantages

✅ High Sensitivity: Orbitrap Fusion™ and TIMS-TOF platforms enable detection of low-abundance succinylated peptides.

✅ Cross-Species Compatibility: Applicable to human, murine, plant, and microbial systems.

✅ End-to-End Support: Streamlined workflows from experimental design to bioinformatic analysis.

✅ Efficiency: Rapid turnaround times to expedite research timelines.

✅ One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

 

Applications

✅ Cancer Biology: Investigating succinylation-driven metabolic reprogramming in tumors.

✅ Drug Discovery: Identifying inhibitors targeting succinylation regulatory enzymes (e.g., SIRT5 inhibitors).

✅ Immunotherapy: Studying succinylation’s role in immune checkpoint modulation.

✅ Neurological Disorders: Linking succinylation aberrations to neurodegenerative disease markers.

✅ Agricultural Biotechnology: Engineering crops or microorganisms via succinylation-based modifications.

 

How Can We Help You?  

1. Communication of Requirements: Fill out an inquiry form for a customized solution.  

2. Sample Submission: We accept dry ice shipments and samples such as cells (1×10⁷), body fluids (>200 µL), and tissues (>50 mg). Contact us for specific requirements.  

3. Experiment and Analysis: Tailored lipidation analysis is completed within 15 business days, with real-time project updates for complex studies.  

4. Report Delivery: Reports include succinylation site identification, quantitative modification data, raw MS files, peptide spectra (MS/MS), and bioinformatics analysis results.  

 

As a leader in PTM analysis, MtoZ Biolabs facilitates translational research by combining methodological rigor with actionable insights. Our customizable succinylation analysis service empowers researchers exploring cancer immunometabolism, targeted therapies, or mechanistic studies. For project-specific consultations or feasibility assessments, contact our team to leverage succinylation analysis for pioneering discoveries.