Myristoylation Analysis Service

Myristoylation analysis service focuses on analyzing the myristoylation modification of proteins, primarily using mass spectrometry to accurately identify the covalent attachment sites of myristic acid on proteins. Myristoylation is a common covalent lipid modification that plays a critical role in cellular signal transduction, membrane localization, and protein-protein interactions. Studying the myristoylation status of proteins is crucial for understanding their functional regulatory mechanisms, especially in disease research and drug development.

 

MtoZ Biolabs, an integrated chromatography and mass spectrometry services provider, provides advanced proteomics, metabolomics, and biopharmaceuticaI analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. We offer high-precision myristoylation analysis service, focusing on the identification and quantification of myristoylation modifications in proteins using mass spectrometry technology. Through our advanced mass spectrometry platform, combined with optimized sample enrichment strategies, we can accurately identify and localize the attachment sites of myristic acid on proteins. Our service covers the entire workflow, from sample preparation to data analysis, ensuring that each step meets high standards, providing highly sensitive and high-resolution modification data to help researchers uncover the biological functions of protein modifications.

 

Analysis Workflow

 



 

Service Advantages

1. High Sensitivity Detection: Utilizing advanced mass spectrometry technology, MtoZ Biolabs efficiently identifies low-abundance myristoylation sites, ensuring precise detection of trace modifications in complex biological samples.

 

2. Accurate Localization of Modification Sites: By combining high-resolution mass spectrometers and optimized analytical methods, we can accurately determine the attachment sites of myristic acid on proteins, providing detailed information on modification sites and aiding in the understanding of protein functional regulation.

 

3. Optimized Sample Enrichment Strategy: MtoZ Biolabs employs precise sample handling and enrichment techniques to ensure the stability and integrity of myristoylation modifications, providing reliable analytical results while preventing modification loss or transformation.

 

4. Comprehensive Data Analysis Support: Leveraging a powerful data processing platform and rich database resources, we provide efficient mass spectrometry data analysis to ensure the accuracy and comprehensiveness of modification information, helping clients thoroughly investigate the impact of myristoylation on protein function and interactions.

 

Sample Submission Suggestions

1. Sample Types: We accept various types of biological samples, including cell lysates, tissue extracts, serum, plasma, culture media, and purified proteins. Depending on the research requirements, clients can provide whole protein samples or enriched phosphorylated peptides.

 

2. Sample Amount: To ensure high sensitivity and high-resolution analysis, we typically require sufficient total protein for each sample. If the sample consists of phosphorylated peptides, an adequate amount should be provided to ensure effective detection and identification.

 

3. Sample Purity: To enhance the accuracy of phosphorylation site identification, it is recommended to provide as pure a protein sample as possible. For complex samples with significant impurities or non-phosphorylated proteins, enrichment or pre-treatment steps may be required, such as immuno-enrichment of phosphorylated proteins.

 

4. Sample Solution Requirements: Samples should be dissolved in a buffer suitable for mass spectrometry analysis, avoiding solutions with high concentrations of salts, sugars, or organic solvents, as these substances may interfere with mass spectrometry analysis. Phosphate-buffered saline (PBS) or other suitable buffers are recommended, and the pH of the sample should be within the range of 7.0-8.0.

 

Sample Results

1. Protein N-Myristoylation Plays a Critical Role in the Endoplasmic Reticulum Morphological Change Induced by Overexpression of Protein Lunapark, an Integral Membrane Protein of the Endoplasmic Reticulum

 



Moriya, K. et al. PLoS One. 2013.

 

Applications

1. Research for Cardiac Hypertrophy and Heart Failure

 



Tomita, Y. et al. JACC Basic Transl Sci. 2023.

 

FAQ

1. While mass spectrometry can help identify modification sites, how can we overcome the ambiguity of modification sites and ensure the precise localization of each site?

To ensure precise localization of each modification site, we are recommended using high-resolution mass spectrometry technologies, such as Orbitrap, to provide more accurate mass information. Additionally, combining data-dependent acquisition (DDA) and data-independent acquisition (DIA) modes would enhance the identification rate of modification sites. We could also utilize specialized mass spectrometry analysis software for data processing, optimizing site validation and localization by comparing against existing protein databases.

 

2. Since myristoylation modification shares similar mass spectrometry characteristics with other lipid modifications, such as palmitoylation, how can we distinguish and accurately analyze the quantity and location of these modifications?

To address the issue of distinguishing myristoylation from other lipid modifications (such as palmitoylation), we can use more detailed mass spectrometry techniques, such as multi-stage mass spectrometry (MSn), to further analyze fragment ions of the modifications. By combining quantitative mass spectrometry techniques (such as SRM or PRM), we can differentiate between various lipid modifications and perform accurate quantification. Furthermore, optimizing the data processing workflow and algorithms, along with using specialized lipid modification databases for comparison, will improve the differentiation of modification sites and types.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Myristoylation Analysis

5. Mass Spectrometry Image

5. Bioinformatics Analysis

6. Raw Data