Lysine 2-hydroxyisobutyrylation Quantitative Analysis Service
Lysine 2-hydroxyisobutyrylation (Khib) is a novel post-translational modification (PTM) characterized by the addition of carboxylic acid-derived groups to lysine residues, significantly altering protein conformation and function. Khib modifications are widely distributed across histone and non-histone proteins and play pivotal roles in regulating the cell cycle, chromatin dynamics, and metabolic pathways. Due to their evolutionary conservation and functional plasticity, Khib marks are emerging as key targets in epigenetics and disease mechanism research.
MtoZ Biolabs offers dedicated Lysine 2-hydroxyisobutyrylation Quantitative Analysis Service that integrates pan-specific anti-Khib antibody enrichment with high-resolution LC-MS/MS analysis. This service enables confident site identification, precise quantitation, and functional insight into Khib-regulated signaling networks, supporting a wide array of biological and biomedical investigations.
Analysis Workflow
To ensure high reproducibility and data quality in our Lysine 2-hydroxyisobutyrylation Quantitative Analysis Service, we have established a well-validated workflow that encompasses the entire process—from sample preparation to in-depth data mining.
1. Protein Extraction and Enzymatic Digestion
Biological samples (cells or tissues) are subjected to lysis, reduction, and alkylation, followed by trypsin digestion to generate peptides suitable for modification enrichment.
2. Khib Peptide Enrichment
Modified peptides are selectively enriched using pan-specific anti-Khib antibodies conjugated to magnetic beads, enhancing downstream sensitivity and selectivity.
3. High-Resolution LC-MS/MS Detection
Enriched samples are analyzed using instruments such as Orbitrap Fusion Lumos in DDA or DIA modes to capture high-confidence fragmentation data.
4. Bioinformatic Identification of Modification Sites
Data are processed through software such as MaxQuant, enabling accurate Khib site assignment with strict quality control filtering.
5. (Optional) Functional Annotation and Pathway Analysis
GO/KEGG enrichment and protein interaction networks can be conducted to reveal the biological relevance of Khib modifications.
Bo F. et al. Scientific Reports. 2021.
Figure 1. Systematic Analysis of Khib in Wheat Roots
Why Choose MtoZ Biolabs?
✅ Ultra-Sensitive Detection
Enabled by Orbitrap Fusion Lumos and Q Exactive HF-X platforms, ensuring high-accuracy identification of low-abundance modified peptides.
✅ High-Specificity Antibody Enrichment
Utilization of pan-Khib antibodies guarantees strong enrichment performance and excellent signal clarity.
✅ Flexible Quantification Strategies
Multiple labeling and acquisition options (DIA, TMT, iTRAQ) available to support varied experimental designs and biological replicates.
✅ Broad Sample Compatibility
Suitable for tissues, cultured cells, microbial samples, biofluids, and IP-derived materials.
✅ Custom Bioinformatics Support
Optional downstream functional interpretation, including pathway enrichment and network visualization.
✅ Expert-Driven Support
Our PTM specialists provide end-to-end consultation and tailored data interpretation to meet your research objectives.
Sample Submission Suggestions
Our Lysine 2-hydroxyisobutyrylation Quantitative Analysis Service supports a wide variety of sample types. Please follow the submission suggestions below:
Sample Types: Tissue lysates, cell lysates, purified proteins, IP products
Recommended Protein Amount: ≥1 mg total protein (BCA quantification recommended)
Storage and Shipping: Store at −80°C and ship on dry ice; avoid repeated freeze-thaw cycles
Note: Please inform us in advance if samples contain MS-inhibitory substances (e.g., detergents, glycerol) so we can adjust preprocessing accordingly. For complete sample preparation instructions, contact MtoZ Biolabs technical support.
Applications
Our Lysine 2-hydroxyisobutyrylation Quantitative Analysis Service empowers researchers across a spectrum of biological questions:
· Global PTM Mapping and Annotation: Systematic identification of Khib-modified proteins and construction of modification atlases.
· Quantitative Differential Analysis: Comparative Khib profiling under different physiological or treatment conditions.
· Epigenetic Regulation Studies: Assessment of Khib marks on histones to elucidate chromatin-level regulatory mechanisms.
· Metabolic Pathway Exploration: Investigation of non-histone Khib roles in central metabolism, including glycolysis and the TCA cycle.
· Disease Mechanism Research and Biomarker Discovery: Linking Khib changes to disease progression in cancer, neurodegeneration, and other pathologies.
What Could be Included in the Report?
1. Raw MS files (.raw or .mzML)
2. Identified Khib site list with localization scores
3. Quantitative data tables and graphical statistics
4. Functional annotation results (GO, KEGG, PPI, as requested)
5. Methodological documentation and QC metrics
Related Services
Protein 2-Hydroxyisobutyrylation Analysis
How to order?
