Label Free Shotgun Proteomics Service

Label Free shotgun proteomics is a high-throughput protein identification and quantification technique that combines liquid chromatography (LC) with high-resolution mass spectrometry (MS) to analyze the protein composition and relative expression levels in complex biological samples, all without the need for external labeling. The "shotgun" approach utilizes random enzymatic digestion to generate peptides, which are then directly separated and detected in the peptide mixture. This strategy greatly enhances both experimental efficiency and the depth of analysis, providing a comprehensive overview of the proteome.

 

Label Free shotgun proteomics offers a powerful solution to the challenge of detecting proteins across a wide dynamic range, especially low-abundance proteins, by leveraging its high sensitivity, broad coverage, and efficient peak intensity-based quantification via mass spectrometry. The absence of labeling significantly reduces experimental costs, streamlines workflows, and is especially suitable for large-scale analyses across multiple sample conditions. This technique enables in-depth proteomic analysis, providing robust data for studying molecular mechanisms, investigating disease-associated protein alterations, and discovering potential biomarkers.

 

MtoZ Biolabs offers high-quality Label Free shotgun proteomics service with state-of-the-art instruments like the Orbitrap Fusion Lumos and Q Exactive HF, supported by a highly experienced team. We support clients through the entire process, from sample preparation to data analysis, ensuring high sensitivity, broad coverage, and precise data, all of which facilitate groundbreaking discoveries in proteomics research.

 

Analysis Workflow

Our optimized workflow for Label Free shotgun proteomics service ensures reproducible and high-quality results. Each step of the process is carried out by a skilled team with rigorous scientific validation, from sample preparation to data analysis.

1. Sample Preparation: Proteins are extracted and enzymatically digested using standardized methods, ensuring sample purity and integrity.
2. Peptide Separation: Efficient nano-liquid chromatography (Nano-LC) systems separate peptides, optimizing resolution and sensitivity.
3. Mass Spectrometry Detection: High-resolution mass spectrometers provide accurate peptide mass spectra, ensuring high sensitivity and precision.
4. Data Analysis: Proteins are identified and quantified using specialized bioinformatics tools and databases, producing reliable and detailed reports.

 

 

Service Advantages

1. Label Free Approach

MtoZ Biolabs offers a label free approach that reduces costs and simplifies workflows, making it ideal for large-scale multi-sample analyses.

 

2. High Sensitivity

Our service enables the detection of low-abundance proteins while ensuring broad coverage of high-abundance proteins, providing comprehensive proteomic profiles.

 

3. Advanced Analysis Platform

MtoZ Biolabs established an advanced Label Free shotgun proteomics platform, guaranteeing reliable, fast, and highly accurate analysis service.

 

4. One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

 

5. High-Data-Quality

Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all proteomics analysis data, providing clients with a comprehensive data report.

 

Sample Submission Suggestions

To ensure optimal performance, sample preparation is crucial. MtoZ Biolabs offers comprehensive guidance to help clients achieve the best experimental conditions, maximizing data accuracy and sensitivity.

• Sample Types: Suitable for a variety of biological samples, including cells, tissues, serum, and plasma.
• Sample Quantity: A recommended protein concentration of 1 μg/μL, with a total protein amount of at least 20 μg per sample.
• Sample Purity: Minimize interference from lipids, polysaccharides, and salts, as these can compromise data quality.

 

Applications

The Label Free shotgun proteomics service enables high-throughput, label-free protein analysis, providing deep insights into biological systems. It is a key tool for investigating disease mechanisms, drug efficacy, and biomarker discovery.

• Basic Research: Investigating protein function and regulatory mechanisms in life sciences.
• Disease Research: Identifying protein changes associated with disease states to uncover pathophysiological mechanisms.
• Drug Development: Screening targets and evaluating therapeutic effects in precision medicine.
• Biomarker Discovery: Identifying key molecules for clinical diagnosis and treatment.

 

Case Study

Case1

Label Free shotgun proteomics identified collagen proteins in soft-shell turtles as regulators of HaCaT cell proteins, supporting research on skin health.

 

 

Yamamoto, T. et al. J. Biomed. Mater. Res. Part B Appl. Biomater. 2017.

 

Case2

Label Free shotgun proteomics was used to identify potential protein biomarkers related to beef tenderness in muscle and plasma.

 

Boudon, S. et al. J Proteomics. 2020.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment

4. Data Analysis, Preprocessing, and Estimation

5. Bioinformatics Analysis

6. Raw Data Files

 

MtoZ Biolabs' Label Free shotgun proteomics service featuring high sensitivity, broad coverage, and precise quantification provides reliable data and comprehensive technical support. Our optimized workflow and expert team ensure the success of every experiment, enabling key breakthroughs in basic research, disease mechanism exploration, and biomarker discovery. Choose MtoZ Biolabs to propel your proteomics research to new heights and unlock limitless possibilities.