Quantitative Peptidomics Service

Quantitative peptidomics analysis is a research approach that utilizes mass spectrometry to systematically and quantitatively analyze endogenous peptides, aiming to reveal changes in peptide expression under different physiological or experimental conditions. This technique employs various quantification strategies to achieve relative or absolute quantification of peptides across multiple samples, and, combined with high-resolution LC-MS/MS platforms, enables the analysis of abundance information for thousands of peptides simultaneously.

Quantitative peptidomics service is widely applied in fields such as neuroscience, immunology, metabolic research, stress response mechanism exploration, and biomarker discovery. Through quantitative peptidomics, the roles of endogenous peptides in processes such as signal transduction, proteolysis, and hormone regulation can be deeply understood, providing critical data support for further basic research.

Services at MtoZ Biolabs

Based on high-resolution mass spectrometry platforms, MtoZ Biolabs offers a quantitative peptidomics service focused on the systematic quantification of peptides. This service can apply various quantification strategies according to specific needs and is suitable for differential expression analysis of peptides in samples such as cells, tissues, and body fluids. Through this service, clients can obtain peptide identification data, relative or absolute abundance information, and potential functional annotations, supporting mechanism studies and biomarker discovery. The services we provide include, but are not limited to, the following:

1. Semi-Quantitative Peptidomics Analysis Service

Compares the relative abundance changes of peptides across different samples based on mass spectrometric signal intensity.

2. MRM-Based Peptide Quantification Service

Uses Multiple Reaction Monitoring (MRM) mode to achieve high-sensitivity quantification of specific peptides.

3. iTRAQ-Based Peptidomics Analysis Service

Applies iTRAQ labeling for relative quantification of peptides across multiple samples, ideal for multi-group comparison.

4. PRM-Based Peptide Quantification Service

Utilizes Parallel Reaction Monitoring (PRM) for accurate detection of target peptides with enhanced specificity and precision.

5. SILAC-Based Peptidomics Analysis Service

Employs metabolic labeling in cells to perform relative quantification of peptides, suitable for in vitro cellular samples.

6. Shotgun Peptidomics Analysis Service

Applies untargeted mass spectrometry to broadly identify and semi-quantify endogenous peptides in complex samples.

7. Peptide Absolute Quantification Service

Combines stable isotope-labeled internal standards with standard curves to provide accurate concentration data of target peptides.

8. Isotope Labeling Peptidomics Analysis Service

Introduces isotopes of different masses to enable quantitative comparison of peptides across multiple samples.

9. TMT-Based Peptidomics Analysis Service

Uses TMT tags to label multiple samples, allowing high-throughput and multiplexed quantitative analysis.

10. Label-Free Peptide Quantification Service

Performs relative quantification by directly comparing peptide signal intensities without the need for external labeling.

11. Isobaric Labeling Peptidomics Analysis Service

Uses equal-mass isobaric tags to simultaneously quantify peptides from multiple samples within a single experiment.

Service Advantages

1. Flexible Quantification Strategies

Offers a variety of quantification methods including Label-free, TMT/iTRAQ, SILAC, and MRM/PRM to meet diverse research needs with flexibility.

2. High Throughput and Sensitivity

Utilizes advanced mass spectrometry platforms such as Orbitrap, enabling sensitive detection of low-abundance peptides and efficient parallel analysis of multiple samples.

3. Comprehensive Data Coverage

Capable of profiling thousands of peptides, providing multidimensional data including identification, quantification, modification sites, and functional annotation.

4. One-Stop Service Workflow

MtoZ Biolabs delivers end-to-end solutions from experimental design and sample analysis to bioinformatics interpretation, ensuring efficient advancement of peptidomics research.

Applications

1. Differential Expression Analysis

By quantitatively comparing peptide abundance under different conditions, this service helps identify key regulatory factors and reveals dynamic biological responses.

2. Post-Translational Modification Research

Quantitative peptidomics service enables precise quantification of modified peptides such as phosphorylation and acetylation, supporting studies on dynamic protein regulation in cellular processes.

3. Biomarker Discovery

Systematic quantification of peptides associated with specific physiological or pathological states provides a basis for biomarker development and functional validation.

4. Signaling Pathways and Protein Degradation Studies

Quantitative peptidomics service can be used to analyze peptide expression trends in signal transduction and proteolysis, aiding in mechanistic investigations.