Peptide Quantification Service

Peptide quantification is a critical approach in proteomics that enables precise measurement of peptide abundance to reflect protein expression levels and dynamic changes under specific biological conditions. By accurately quantifying peptides, researchers gain valuable insights into protein regulation, cellular pathways, and post-translational modifications (PTMs), such as phosphorylation or acetylation. Peptide quantification plays an essential role in understanding cellular processes, identifying disease biomarkers, and facilitating drug development. Advanced technologies now allow high-throughput peptide quantification with exceptional sensitivity and accuracy, even in complex biological samples.

 

Service at MtoZ Biolabs

MtoZ Biolabs delivers Peptide Quantification Service with high resolution and sensitivity, leveraging cutting-edge mass spectrometry platforms. MtoZ Biolabs’ Peptide Quantification Service starts with biological specimen collection, followed by protein extraction, enzymatic digestion, and peptide preparation. Using high-resolution LC-MS/MS platforms, we generate raw data that undergoes rigorous database searches and FDR control to ensure high-confidence peptide identification. Quantification is achieved through relative or absolute measurement strategies, comparing signal intensities to determine peptide abundances across different conditions. Identified peptides are further mapped to their corresponding proteins, allowing for protein inference and comprehensive biological interpretation. Results are presented as precise peptide quantities, providing insights into protein expression dynamics, post-translational modifications, and biological pathways. 

 



 

Shuken, S. R. J Proteome Res. 2023.

 

Figure 1. Workflow for Peptide Quantification Service

 

Our Peptide Quantification Service supports a variety of quantification strategies to meet diverse research needs. We provide label-based strategies, including SILAC, TMT/iTRAQ, and ICAT, for high-precision and multiplexed quantification. For cost-effective and large-scale studies, our label-free quantification (LFQ) delivers reliable results without sample labeling. Additionally, we specialize in targeted quantification using MRM/SRM and PRM for accurate measurement of specific peptides. For absolute quantification, we utilize stable isotope-labeled synthetic peptides as internal standards to ensure precise peptide abundance determination. By combining robust workflows and stringent quality control, our Peptide Quantification Service provides reproducible and accurate data to advance your research.

 



 

Shuken, S. R. J Proteome Res. 2023.

 

Figure 2. Peptide Quantification Methods

 

Service Advantages

1. Advanced Analysis Platform: MtoZ Biolabs established an advanced Peptide Quantification Service platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. Comprehensive Quantification Solutions: MtoZ Biolabs offers a full range of peptide quantification strategies, including label-free, label-based (SILAC, TMT/iTRAQ), targeted methods (MRM/SRM, PRM), and more to meet diverse research needs. 

3. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

4. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all Peptide Quantification Service data, providing clients with a comprehensive data report.

5. Fast Turnaround: The process from sample handling to report generation is efficient, shortening the analysis cycle.

 

Applications

Applications of Peptide Quantification Service span multiple research areas, including disease mechanism studies, drug target analysis, post-translational modification quantification, and biomarker discovery. For example, identifying differentially expressed peptides in tumor tissues can reveal critical biomarkers for cancer diagnosis and prognosis. Similarly, quantifying PTM-specific peptides provides insights into regulatory mechanisms in signaling pathways.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Peptide Quantification

5. Mass Spectrometry Image

6. Raw Data

 

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our Peptide Quantification Service is designed to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details.