Differential Peptide Expression Analysis Service

Differential peptide expression analysis is a systematic research approach designed to compare differences in endogenous peptide expression across multiple biological samples, revealing potential biological regulatory mechanisms, protein degradation pathways, and disease-related molecular events. This analysis is crucial for exploring cellular response states, discovering disease biomarkers, and evaluating therapeutic effects.

Unlike proteomics, which focuses on larger proteins, peptidomics concentrates on functional or regulatory peptides generated after protein degradation. These small peptides change rapidly and are highly sensitive, making them ideal for tracking subtle dynamics in complex biological processes. MtoZ Biolabs offers both standardized and customized Differential Peptide Expression Analysis Service that leverages years of experience in mass spectrometry analysis and bioinformatics to provide precise and reliable support for life sciences, pharmaceutical, and clinical research worldwide.

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Puchalski, M. et al. Int. J. Mol. Sci. 2024.

Figure 1. Peptide Expression Comparison Across Tumor and Healthy Tissue Samples

Services at MtoZ Biolabs

MtoZ Biolabs uses the Thermo Fisher Q Exactive HF and Orbitrap Fusion Lumos high-resolution mass spectrometry systems, combined with nano-liquid chromatography (Nano-LC), to create a stable and highly sensitive differential peptide expression analysis platform. We apply various quantification methods, including Label-Free, TMT, and iTRAQ, to meet the diverse needs of different experimental designs and groupings. Our data processing stage uses statistical models (e.g., t-tests, ANOVA, multiple hypothesis correction) to identify significantly different peptides. We further analyze these peptides through GO annotations, KEGG pathway enrichment, and protein interaction network analysis to explore their biological functions in disease mechanisms, signaling pathways, and protein regulation.

Analysis Workflow

Our Differential Peptide Expression Analysis Service follows a standardized six-step workflow, ensuring accurate execution of each experimental step and high-quality data:

1. Sample Collection

We collect representative samples that meet the biological requirements of the experiment, ensuring reliability for subsequent analysis.

2. Peptide Enrichment

Ultrafiltration and solid-phase extraction are used to enrich peptides smaller than 10 kDa, removing high-molecular-weight impurities to enhance the sensitivity and accuracy of mass spectrometry.

3. Peptide Identification and Quantification

We use high-resolution mass spectrometry to accurately identify peptides, measuring their expression levels with techniques like TMT, SILAC, or Label-Free.

4. Differential Analysis and Data Interpretation

Statistical analysis identifies significant peptide expression differences between experimental groups. Bioinformatics tools are used to perform functional enrichment, pathway analysis, and network construction, revealing the biological roles of differentially expressed peptides.

5. Report and Data Visualization

We provide detailed analysis reports with quantitative data, statistical charts (e.g., heatmaps, volcano plots, PCA), ensuring results are easy to interpret and ready for further application.

Why Choose MtoZ Biolabs?

✔ Top-tier Mass Spectrometry Platforms for High Sensitivity

We use state-of-the-art systems like Orbitrap, Exploris 480, and TimsTOF to ensure accurate peptide identification and sensitive quantification, ideal for low-abundance peptide analysis.

✔ Multiple Quantification Methods for Enhanced Accuracy

TMT, SILAC, and Label-Free technologies are applied for precise quantification of peptide expression differences, ensuring the reliability of our data.

✔ Support for a Wide Range of Samples

We accommodate various sample types, including tissues, cells, plasma, cerebrospinal fluid, and more, catering to research needs in cancer, metabolic diseases, and beyond.

✔ Comprehensive Data Interpretation for Mechanism Research

We provide extensive data interpretation through bioinformatics and functional enrichment, helping clarify the role of different peptides in disease processes.

✔ Efficient Visualization for Clearer Results

Our data visualization tools, including heatmaps and volcano plots, provide clear insights into your research, making the results easy to understand and apply.

Sample Submission Suggestions

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Note: Provide details on sample collection and handling. If you need further details, our technical support team is happy to assist and provide comprehensive guidance on sample submission.

Applications

The Differential Peptide Expression Analysis Service is ideal for various research areas:

· Disease Mechanism Analysis: Comparing peptide expression differences in diseased vs. normal tissues to identify key pathway abnormalities.

· Drug Intervention Evaluation: Analyzing peptide changes before and after treatment to evaluate drug target activation or inhibition.

· Biomarker Discovery: Identifying peptides with specific expression patterns for early diagnosis or efficacy prediction.

· Species and Tissue Differences: Exploring peptide expression differences across species or tissues and understanding their regulatory mechanisms.

· Integrative Omics Research: Combining peptidomics with proteomics and metabolomics to provide deeper system-level insights.

MtoZ Biolabs’ Differential Peptide Expression Analysis Service offers advanced analysis technology and professional support, helping you make breakthroughs in studying biological processes, disease mechanisms, and biomarker discovery. Whether researching disease mechanisms, drug development, or functional peptides, we offer customized, precise solutions. Contact us for more information and professional service support.