Peptide Absolute Quantification Service

Peptide absolute quantification analysis is a mass spectrometry-based method aimed at accurately measuring the concentration of specific peptides, widely used in protein quantification, biomarker validation, and metabolic research. This service introduces synthetic peptides labeled with stable isotopes as internal standards, combined with high-sensitivity mass spectrometry platforms, and performs analysis using multiple reaction monitoring (MRM) or parallel reaction monitoring (PRM) modes. By establishing a standard curve, it precisely determines the absolute concentration of target peptides in biological samples, supporting high-precision quantification for various biological sample types.

 

The peptide absolute quantification service is widely applied in biomarker validation, metabolic research, and sample quality control. Compared to relative quantification methods, peptide absolute quantification offers advantages such as higher sensitivity, stronger accuracy, and traceability, making it suitable for key stages of quantitative verification of specific proteins or modification sites.

 

Services at MtoZ Biolabs

Based on high-sensitivity mass spectrometry platforms, MtoZ Biolabs offers the peptide absolute quantification service focusing on precise and absolute quantification of target peptides. This service uses stable isotope-labeled peptides as internal standards, combined with MRM or PRM modes for detection, making it suitable for absolute quantification of peptides in complex biological samples. Through this service, clients can obtain accurate concentration information for specific peptides, supporting applications such as biomarker validation, metabolic research, and modification site verification.

 

Analysis Workflow

1. Peptide Design

Based on the target peptide sequence, corresponding stable isotope-labeled peptides are synthesized and accurately prepared into standard concentrations for subsequent quantification analysis.

 

2. Sample Mixing

The peptide sample to be analyzed is mixed with internal standard peptides of known concentration to ensure that both the endogenous peptides and internal standards are detected within the same analysis system.

 

3. LC-MS/MS Analysis

High-efficiency liquid chromatography is coupled with high-sensitivity mass spectrometry to simultaneously monitor the characteristic ion pairs of the target peptides and internal standard peptides in MRM or PRM modes.

 

4. Data Processing and Quantification Calculation

The absolute concentration of the target peptides in the sample is calculated based on the internal standard signal intensity and the standard curve, ensuring accurate and traceable results.

 

5. Result Output and Reporting

A comprehensive analysis report is provided, including quantification results, standard curves, mass spectrometry spectra, and method parameters, meeting the needs of both research and application.

 

Sample Submission Suggestions

1. Sample Type

Accepted peptide samples should be digested and purified, suitable for a variety of sources including cells, tissues, serum, plasma, urine, and more.

 

2. Sample Storage and Transport

Samples should be stored at low temperatures, and transported with dry ice to prevent degradation. Repeated freeze-thaw cycles should be avoided to maintain the integrity of the peptides and ensure accurate quantification results.

 

3. Additional Information

It is recommended to provide sample preprocessing information and target peptide sequences (if available) to optimize internal standard design and mass spectrometry parameter settings.

 

Service Advantages

1. High-Resolution Mass Spectrometry Platform

Using advanced mass spectrometry instruments, we provide high-quality, high-resolution quantification data, ensuring the accuracy and stability of the quantitative results.

 

2. High Sensitivity and Broad Coverage

By adopting MRM or PRM modes, this service enables precise detection of low-abundance peptides in complex samples, suitable for quantitative analysis across various biological sample types and abundance ranges.

 

3. Customized Analysis Solutions

We support clients in customizing target peptide sequences and provide personalized quantification solutions, including internal standard peptide design, standard curve establishment, and parameter optimization, to meet diverse research objectives.

 

4. One-Stop Quantification Service

MtoZ Biolabs offers a comprehensive service workflow, covering peptide configuration, sample analysis, data processing, and report interpretation, helping clients efficiently conduct high-quality quantitative research.

 

Applications

1. Biomarker Quantification Validation

The peptide absolute quantification service can be used to accurately measure the absolute abundance of specific peptides under different physiological conditions, providing reliable data support for biomarker screening and functional studies.

 

2. Protein Expression Level Assessment

By quantifying target peptides, this service reflects changes in protein expression, making it suitable for research on cellular regulation, metabolic states, and response mechanisms.

 

3. Precise Quantification of Post-Translational Modifications (PTMs)

The peptide absolute quantification service can be used for absolute quantification of peptides at specific modification sites such as phosphorylation and acetylation, revealing the role of these modifications in signal transduction and functional regulation.

 

4. Standard Curve Methodology Establishment and Validation

This service is used to establish standard curves and quantification methods for specific peptides, validating the accuracy and reproducibility of quantification strategies, supporting the optimization and standardization of experimental systems.

 

FAQ

Q1: What Is the Difference between Absolute Quantification and Relative Quantification?

A1: Relative quantification can only compare the relative expression differences of peptides between different samples, whereas absolute quantification directly measures the actual concentration of peptides in the sample, making it suitable for precise measurement of specific targets and cross-sample data comparison.

 

Q2: Can Multiple Peptides Be Detected?

A2: Yes, we support the absolute quantification of multiple target peptides simultaneously. It is important to plan the internal standard configuration and method parameters in advance to ensure that each peptide has good specificity and response.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Data Analysis, Preprocessing, and Estimation

4. Bioinformatics Analysis

5. Raw Data Files