TMT-Based Peptidomics Analysis Service

Tandem mass tag (TMT) labeling is a mass spectrometry-based quantitative analysis technique widely used in peptidomics research. Its basic principle involves labeling peptides from different samples with isotopic tags (TMT tags), rendering them indistinguishable in mass during MS analysis. Quantitative analysis is then performed on the reporter ions of peptide tags during the MS/MS stage, enabling parallel comparison across multiple samples and providing high-precision quantitative data. This technique allows high-throughput analysis of peptides from different samples and is suitable for quantitative comparison studies involving multiple conditions.

 

TMT-based peptidomics analysis service is widely applied in fields such as cell biology, metabolomics, biomarker discovery, and drug development. It is particularly suitable for quantitative analysis of peptides in complex samples, enabling the detection of differential peptide expression under various treatments or time points. This provides robust data support for exploring biological mechanisms, pharmacodynamic studies, and clinical diagnostics.

 

Services at MtoZ Biolabs

Based on a high-resolution mass spectrometry platform, the TMT-based peptidomics analysis service launched by MtoZ Biolabs focuses on quantitative analysis of peptide samples. This service utilizes TMT isotopic labeling technology to label peptides from different samples, enabling parallel quantitative analysis across multiple conditions. Clients can obtain high-throughput and accurate quantitative data, making it suitable for analyzing peptide expression differences in complex biological samples. It is widely applied in biomarker discovery, functional peptide research, and drug development.

 

Analysis Workflow

1. Sample Preparation and Peptide Extraction

Proteins are extracted from biological samples and enzymatically digested to generate peptides, preparing them for downstream analysis.

 

2. TMT Labeling

TMT reagents are used to isotopically label different samples, enabling multiplexed quantitative analysis during mass spectrometry.

 

3. Liquid Chromatography–Mass Spectrometry Analysis (LC-MS/MS)

Labeled peptides are separated and quantitatively analyzed using a high-resolution mass spectrometry platform combined with a liquid chromatography system.

 

4. Data Processing and Quantitative Analysis

Mass spectrometry data are processed using specialized software to calculate quantitative ratios of peptides and generate expression profiles across multiple samples.

 

5. Result Interpretation and Report Delivery

A detailed data report is provided, including peptide quantification results, differential analysis, and functional annotation for subsequent research use.

 



Neagu, A N. et al. Molecules, 2022.

Figure 1. Overview of Analysis by Tandem Mass Spectrometry.

 

Sample Submission Suggestions

1. Sample Types

A wide range of biological sample types is supported, including serum, plasma, tissue homogenates, and cell lysates. Samples should be provided in sufficient quantity and with ensured protein extraction quality to guarantee the accuracy of subsequent analyses.

 

2. Sample Storage and Transportation

Samples should be stored at −80°C and transported on dry ice to prevent repeated freeze-thaw cycles that could affect mass spectrometry analysis.

 

3. Sample Information

Detailed background information on the samples should be provided, including sample origin, processing methods, and whether exogenous labeling was applied, to help optimize the analysis workflow and ensure data accuracy.

 

Service Advantages

1. High Throughput and High Precision

By employing TMT labeling technology, multiple samples can be analyzed simultaneously with high throughput, and mass spectrometry enables the acquisition of high-precision quantitative data, making it suitable for peptide analysis in complex biological samples.

 

2. High Sensitivity and Accuracy

TMT labeling combined with mass spectrometry technology allows for accurate detection of changes in low-abundance peptides, ensuring both sensitivity and accuracy of the quantitative results.

 

3. Flexible Customized Solutions

Personalized experimental design solutions are provided based on the client's research needs, including sample grouping, labeling strategies, and data analysis modules, to meet the requirements of research across various fields.

 

4. One-Stop Service

A full-process service is offered, covering sample preparation, TMT labeling, mass spectrometry analysis, data processing, and biological interpretation, simplifying experimental procedures and improving research efficiency.

 

Applications

1. Multi-Sample Comparative Analysis

TMT labeling technology is widely applied to multi-sample comparisons between different experimental groups, enabling the analysis of relative abundance changes of peptides under varying conditions. It is suitable for dynamic changes and time-series research scenarios.

 

2. Cell Signaling Pathway Research

TMT-Based Peptidomics Analysis Service can be used to analyze key peptide changes in cellular signaling pathways, helping to reveal cellular functional regulation and its biological roles.

 

3. Biomarker Discovery

Through TMT-labeled peptidomics analysis, researchers can efficiently identify potential disease-associated biomarkers, providing a foundation for early diagnosis and personalized therapy.

 

4. Protein Function and Post-Translational Modification Research

TMT-Based Peptidomics Analysis Service can be used to explore peptide functions, especially for investigating the impact of post-translational modifications (PTMs) on peptide expression and function, facilitating a deeper understanding of protein regulatory mechanisms.

 

FAQ

Q1: Is TMT Labeling Applicable to All Types of Peptides?

A1: TMT labeling is applicable to most peptides, especially those containing lysine and N-terminal residues. Different peptides and samples may vary in labeling efficiency, so for peptides that are difficult to label, it may be necessary to optimize experimental conditions. We recommend providing relevant sample information to develop the optimal experimental plan.

 

Q2: Does this Service Support the Analysis of Post-Translationally Modified (PTM) Peptides?

A2: We support the analysis of post-translationally modified (PTM) peptides, such as phosphorylation, acetylation, and glycosylation. By incorporating PTM enrichment strategies, the detection sensitivity and accuracy of these modified peptides can be improved.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Data Analysis, Preprocessing, and Estimation

4. Bioinformatics Analysis

5. Raw Data Files