High-Throughput Glycan Screening Service
- UHPLC-FLD-Based High-Throughput Glycan Screening
- Hydrophilic Interaction Chromatography-Based High-Throughput Glycan Screening
- Automated LC-MS-Based High-Throughput Glycan Screening
- MALDI-TOF-MS-Based High-Throughput Glycan Screening
- CE-MS-Based High-Throughput Glycan Screening
- ESI-MSn-Based High-Throughput Glycan Screening
High-throughput glycan screening is a strategy that utilizes automated platforms and multidimensional separation-detection techniques to conduct systematic, rapid, and reproducible structural and functional analyses of glycans in a large number of samples within a short period of time. Glycans, as critical modifications of proteins, lipids, and cell surfaces, play essential roles in cell recognition, signal transduction, immune regulation, and disease progression. Due to their structural complexity and heterogeneity, glycan research has become an indispensable area in life sciences and biomedicine. High-throughput screening not only accelerates basic research but also has broad applications in drug development, disease biomarker discovery, and biopharmaceutical quality control.
Büll, C. et al. STAR Protoc. 2020.
Figure 1. Cell-Based Glycan Arrays
Equipped with advanced mass spectrometry, chromatography, and automated analytical platforms, MtoZ Biolabs is committed to providing a high-quality High-Throughput Glycan Screening Service to both research and industrial clients. Our service helps customers efficiently obtain reliable glycomics data, supporting scientific advancement and practical applications.
Services at MtoZ Biolabs
MtoZ Biolabs’ high-throughput glycan screening service integrates multidimensional separation and multi-platform detection strategies to achieve comprehensive characterization of glycan composition, structure, and modifications. Our service offerings include:
1. HPLC-Based High-Throughput Glycan Screening
2. Mass Spectrometry-Based High-Throughput Glycan Screening
3. High-Throughput N-Glycan Screening
Leveraging advanced analytical platforms and extensive glycobiology expertise, MtoZ Biolabs provides a systematic high-throughput N-glycan screening service covering everything from initial screening to validation, helping to advance scientific and industrial projects efficiently.
4. High-Throughput Glycan Derivatives Screening
Backed by advanced analytical platforms and extensive glycoscience expertise, MtoZ Biolabs provides a comprehensive high-throughput glycan derivatives screening service to efficiently support both research and industrial programs.
Analysis Workflow
1. Sample Preparation: Purification, glycan release, and derivatization of samples.
2. Separation Analysis: Glycan separation using HPLC, CE, and related techniques.
3. Mass Spectrometry Detection: High-resolution detection by MALDI-TOF MS or ESI-MS.
4. Data Analysis: Structural identification and quantification using databases and software.
5. Result Delivery: Comprehensive reports including raw data and analytical results.
Service Advantages
1. Comprehensive Multi-Platform Integration
MtoZ Biolabs is equipped with a variety of separation and mass spectrometry platforms, enabling flexible combinations according to sample characteristics. From basic glycan profiling to complex isomer analysis, we provide comprehensive solutions for diverse research needs.
2. High-Throughput and Reproducibility
Through automation and standardized workflows, hundreds to thousands of samples can be analyzed in parallel within a single batch. This ensures reproducibility and consistency of results, making the high-throughput glycan screening service suitable for large-scale projects.
3. Customized Service
Recognizing the diverse goals of research and industry projects, MtoZ Biolabs offers highly flexible customization. Whether the focus is on glycan structural elucidation, isomer discrimination, or large-scale quantitative comparisons, workflows and data outputs can be tailored to meet specific research objectives.
4. Transparent Pricing
Applications
✅ Disease Biomarker Discovery: Identifying glycan modifications associated with diseases.
✅ Drug Development: Supporting candidate drug screening and glycan–protein interaction studies.
✅ Biopharmaceutical Quality Control: Ensuring glycosylation consistency and product traceability.
✅ Food and Nutrition Research: Analyzing glycans in food to evaluate their impact on quality and physiological function.
✅ Basic Life Science Research: Exploring glycan roles in signal transduction and immune regulation.
FAQs
Q1: What types of glycans can be screened by the high-throughput glycan screening service?
A1: The service covers N-glycans, O-glycans, and a variety of complex oligosaccharides, suitable for serum, cell, tissue, and other biological samples.
Q2: How much sample is required?
A2: Typically, tens to hundreds of micrograms of protein or glycan are required, depending on the sample type and experimental design.
Q3: Do you provide customized analysis?
A3: Yes. Screening schemes can be tailored based on client research objectives, including depth of quantification, focus of structural analysis, and data presentation formats.
Sample Submission Suggestions
1. Sample Types: Protein samples, serum/plasma, cells, tissues, or purified glycans.
2. Sample Amount: At least 50 µg of protein or an equivalent amount of glycans is recommended.
3. Storage and Shipping: Samples should be stored at –80°C and shipped on dry ice.
4. Pre-Submission Consultation: We recommend discussing sample conditions and project goals in advance to optimize experimental design.
Deliverables
1. Raw mass spectrometry data files
2. Glycan composition and structural annotation results
3. Quantitative matrices and statistical data
4. Visualized charts (e.g., glycan distribution maps, abundance plots)
5. Complete experimental and analytical reports
High-throughput glycan screening is a powerful tool for advancing glycomics research and its biomedical applications. Leveraging advanced analytical platforms and standardized workflows, MtoZ Biolabs provides reliable and efficient high-throughput glycan screening service to both research and industrial clients.
If you have related requirements, please contact us. We will design a tailored experimental plan and provide comprehensive technical support to ensure the success of your research and projects.
Related Services
High-Throughput N-Glycan Screening Service
High-Throughput Glycan Derivatives Screening Service
High-Throughput Glycan-Binding Proteins Screening Service
High-Throughput Glycoengineered Cell Line Glycan Screening Service
How to order?
