High-Throughput Glycan Derivatives Screening Service
High-throughput glycan derivatives screening leverages automation and multi-platform integrated analytics to concurrently evaluate hundreds to thousands of glycan derivatives in a single experiment, profiling their binding to biological targets, structural differences, and functional associations. Glycan derivatives include naturally occurring glycan modification products, synthetically engineered variants, and chemically or enzymatically functionalized glycans. These molecules play key roles in protein folding and stability, cell signaling, immune recognition, pathogen infection, and disease progression.
Chao, Q. et al. Front Chem. 2020.
Figure 1. Chemo-Enzymatic Methods for the Synthesis of N-glycan Derivatives
Different classes and modification patterns of glycan derivatives not only reflect cellular metabolic states but are also closely associated with cancer progression, immune dysregulation, metabolic disorders, and congenital disorders of glycosylation. Systematic, high-throughput analysis of structures and interactions enables the discovery of disease-related glyco-biomarkers, optimization of glycoform consistency in biopharmaceuticals, and identification of glycan-dependent molecular targets. Traditional approaches are often constrained by structural heterogeneity, diverse linkages, and complex modifications, resulting in limited throughput, speed, and coverage. High-throughput screening overcomes these limitations through array-based assays, automated enrichment, and multi-dimensional readouts, delivering binding profiles, differential distribution, and functional association data from a single run and markedly accelerating glycoscience research.
Backed by advanced analytical platforms and extensive glycoscience expertise, MtoZ Biolabs provides a comprehensive High-Throughput Glycan Derivatives Screening Service to efficiently support both research and industrial programs.
Services at MtoZ Biolabs
MtoZ Biolabs integrates multiple assay platforms and strategies to rapidly characterize the specific interactions between glycan derivatives and proteins, antibodies, cells, or other molecules, while providing structural context and quantitative analysis. Core strategies include:
Table 1. The Core Strategies for High-Throughput Glycan Derivatives Screening at MtoZ Biolabs
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Immobilize diverse glycan derivatives on solid supports and detect binding with fluorescent probes to map large-scale specificity profiles. |
Bead-Based Screening Couple glycan derivatives to microbeads and react in solution with targets; quantify binding by ELISA or flow cytometry. |
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Fluorescent Probe–Based Screening Use fluorescently labeled glycan probes to assess binding to targets, particularly suitable for cell-level studies. |
Label-Free Kinetics (SPR/BLI) Monitor binding in real time to obtain kinetics and affinity parameters without fluorescent or chemical labels. |
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Mass Spectrometry–Based Screening Use MALDI-TOF or LC-MS/MS to analyze bound glycan derivatives for structural features and relative quantitation. |
Analysis Workflow
1. Consultation and Experimental Design: Define objectives, select optimal platforms, and craft a tailored plan.
2. Sample Preparation and QC: Prepare and verify the quality of glycan probes and/or target molecules.
3. Automated High-Throughput Execution: Run the screening on the selected platforms with automated workflows.
4. Data Acquisition and Analysis: Process signals under standardized pipelines; perform statistics and differential analyses.
5. Reporting and Support: Deliver a detailed report, visualizations, and next-step recommendations.
Service Advantages
1. Multi-Platform Integration and Flexible Combinations
Microarray, bead, fluorescent-probe, SPR/BLI, and MS platforms can be flexibly combined to match research goals, sample properties, and throughput needs.
2. Automation with Traceable Quality
Automated systems with standardized QC checkpoints and analysis pipelines reduce human error, improve batch-to-batch consistency, and ensure full traceability—ideal for long-term or multi-batch studies.
3. Expandable Glycan Derivative Library
Broad coverage of derivative types and modifications; custom incorporation of linkages, functional groups, or labels to dissect binding mechanisms and functional properties.
5. Predictable Timelines
Routine screening can be completed in 2–3 weeks under standard conditions, supporting rapid project advancement.
Applications
1. Mechanistic studies of glycan derivative–receptor interactions
2. Vaccine antigen design and optimization using glycan derivatives
3. Discovery of cancer and metabolic disease–related glycan derivative biomarkers
4. Exploration of glycan–glycan interactions and glycomimetic drug target identification
5. Specificity profiling of antibodies or receptors toward glycan derivatives
Sample Submission Suggestions
1. Protein Samples: ≥ 100 µg, > 90% purity; lyophilized or in low-salt buffer.
2. Antibodies/Cell Lysates: Indicate source, concentration, and storage conditions.
3. Client-Provided Glycan Derivatives: Provide structure, purity, and solvent details.
4. Other Sample Types: Please contact us in advance to confirm compatibility.
Deliverables
1. Description of screening platforms and workflows
2. Raw data files and processed data tables
3. Visualized results (heatmaps, binding profiles, etc.)
4. Statistical analyses and signal evaluation report
5. Comprehensive technical report (methods, conditions, and interpretation)
Glycan derivatives are not only integral components of cell-surface glycans but also pivotal connectors between structural information and functional regulation. With multi-platform integration and automated high-throughput capability, MtoZ Biolabs rapidly reveals biologically meaningful glycan-mediated interactions to speed both discovery research and translational applications.
Contact us to design a customized high-throughput glycan derivatives screening service for your project.
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