High-Throughput Glycan-Binding Proteins Screening Service
Glycan-binding proteins (GBPs) are a class of proteins that recognize and bind to specific glycan structures, playing crucial roles in biological processes such as cell signaling, pathogen recognition, immune regulation, and tumor progression. Through interactions with glycans, different GBPs regulate cell adhesion, communication, and metabolic networks, making them important entry points for understanding life processes and identifying disease targets.
With the advancement of glycoscience and glycomics, researchers have shown increasing interest in GBPs. However, due to the complexity and diversity of glycan structures, traditional detection methods are limited in throughput and coverage, making it difficult to meet the demands of systematic studies. The emergence of high-throughput glycan-binding proteins screening provides researchers with the ability to rapidly and comprehensively analyze the interactions between GBPs and diverse glycans. This approach not only improves research efficiency but also expands the analytical depth of glycan-binding networks, laying the foundation for drug development and biomarker discovery.
Purohit, S. et al. Nat Commun, 2018.
Figure 1. Multiplex Glycan Bead Array for High-throughput Analyses of Glycan-binding Proteins
As a CRO service provider dedicated to high-throughput screening, MtoZ Biolabs leverages advanced mass spectrometry platforms and systematic experimental design to deliver high-quality High-Throughput Glycan-Binding Proteins Screening Service for both research and industrial clients. Our goal is to help clients gain in-depth insights into the functions and mechanisms of GBPs, thereby driving both fundamental research and clinical translation.
Services at MtoZ Biolabs
The high-throughput glycan-binding proteins screening service integrates glycan libraries, automated experimental systems, and advanced detection platforms to systematically analyze the binding characteristics between large sets of GBPs and diverse glycans. By combining multi-dimensional detection data, this approach reveals the binding profiles, affinities, and specificities of GBPs, providing reliable data support for downstream functional studies and applications.
MtoZ Biolabs offers the following main screening strategies:
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Glycan microarrays immobilize diverse glycan structures on chip surfaces, allowing simultaneous detection of GBP binding signals. This approach quickly provides binding profiles and specificity and is currently the most widely used high-throughput method. |
Bead-Based Screening Using beads or magnetic particles conjugated with glycans, this method enables interactions with GBPs in solution. Signals are read via flow cytometry or mass spectrometry, offering multiplexed detection and high flexibility. |
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Fluorescent Probe–Based Screening Fluorescently labeled glycans or proteins generate detectable signal changes upon binding, enabling rapid and sensitive monitoring of GBP interactions. This method is suitable for preliminary screening. |
Label-Free Kinetics (SPR/BLI) Techniques such as SPR and BLI provide real-time monitoring of GBP-glycan binding and dissociation processes. They not only detect binding events but also yield affinity and kinetic parameters, making them valuable in drug development studies. |
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Mass Spectrometry–Based Screening Mass spectrometry can directly detect GBP–glycan complexes, offering molecular weight and structural information. This strategy is especially suited for studying binding properties and binding sites in complex systems. |
Analysis Workflow
1. Sample preparation and quality assessment: Initial quality check and concentration evaluation of submitted samples.
2. Glycan array/library construction: Establish glycan detection platforms tailored to research objectives with broad coverage.
3. Protein–glycan interaction detection: Record binding signals using high-throughput screening systems.
4. Data acquisition and processing: Capture and normalize signals with advanced data processing software.
5. Results analysis and reporting: Perform statistical analysis and database comparison to generate intuitive binding profiles and explanatory reports.
Service Advantages
1. Comprehensive screening coverage
By integrating diverse glycan arrays with highly sensitive detection platforms, MtoZ Biolabs provides broad coverage of glycan structures. This ensures that researchers can obtain a full binding landscape while minimizing omissions or bias.
2. Qualitative and quantitative detection
Our high-throughput glycan-binding proteins screening service identifies GBP binding profiles and delivers quantitative data on binding strengths. This dual capability supports both exploratory and application-oriented research.
3. Compatibility with multiple sample types
The high-throughput glycan-binding proteins screening service supports purified proteins, natural samples, antibodies, and cell lysates, ensuring flexible solutions tailored to different research requirements.
4. Customized solutions
MtoZ Biolabs offers highly customizable screening services. Clients can select glycan library scope, detection method (e.g., microarray, bead-based, mass spectrometry), and output formats. We design tailored screening strategies for disease-related glycans, drug development, or mechanistic studies, ensuring both systematic coverage and targeted depth.
5. Professional data interpretation
Beyond raw data, we provide professional interpretive reports incorporating statistical analysis and literature context. Reports include intuitive binding maps and scientifically grounded explanations, helping researchers transform experimental data into meaningful conclusions.
Applications
1. Basic research: Exploring GBP functions in cell signaling, immune regulation, and pathogen recognition.
2. Disease mechanism studies: Elucidating GBP roles in cancer, infections, and autoimmune diseases.
3. Drug development: Evaluating the binding features of candidate drugs or antibodies to glycans, aiding in screening and optimization.
4. Biomarker discovery: Identifying disease-associated GBP binding patterns to support clinical diagnosis and prognosis.
5. Biopharmaceutical quality control: Monitoring antibody and glycoprotein drug binding properties to ensure production consistency and safety.
FAQs
Q1: Can results be used for quantitative comparison?
A1: Yes. Our platforms provide both qualitative binding profiles and quantitative binding strength data, enabling comparisons across different conditions.
Q2: How long is the typical project timeline?
A2: Standard projects are generally completed within several weeks, depending on sample number and project complexity.
Sample Submission Suggestions
1. Sample types: Purified proteins, recombinant proteins, antibodies, serum, or cell lysates.
2. Sample purity: ≥90% purity recommended; avoid high levels of contaminants.
3. Concentration and volume: Typically 100–500 µg of protein required, depending on project design.
4. Storage and transport: Short-term storage at low temperature; long-term storage at −80°C. Ship on dry ice and avoid repeated freeze–thaw cycles.
For special sample types or storage conditions, please contact us in advance.
Deliverables
1. Raw experimental data files.
2. Processed binding profiles and quantitative data tables.
3. Graphical results (e.g., binding profile maps, affinity curves).
4. Professional interpretive report, including result analysis and supporting references.
The high-throughput glycan-binding proteins screening service offers an efficient and systematic solution for studying glycan–protein interactions. With established experimental platforms and an expert scientific team, MtoZ Biolabs delivers reliable data and scientific insights, empowering both research and industrial applications.
If you are interested in our service, please feel free to contact us. We look forward to collaborating with you to advance glycoscience research and applications.
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High-Throughput Glycan Screening Service
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