High-Throughput N-Glycan Screening Service
N-glycans are oligosaccharide structures linked to the asparagine residues of proteins via N-amide bonds. They are widely present in eukaryotes and are key factors in determining biological processes such as protein folding, stability, cell recognition, signal transduction, and immune regulation. Different types of N-glycan modifications not only reflect the metabolic state of cells but are also closely related to the occurrence and development of various diseases, including tumor progression, pathogen infection, inflammatory responses, and congenital glycosylation disorders.
In basic research and applied development, systematically analyzing the structural features and distribution patterns of N-glycans is of great significance for discovering disease-related glycan biomarkers, optimizing glycoform consistency in biopharmaceuticals, and revealing glycan-dependent molecular interaction mechanisms. However, due to their high heterogeneity, complex structures, and diverse modification patterns, traditional analytical methods are often limited in sample throughput, detection speed, and diversity coverage. High-throughput N-glycan screening technology, through array-based detection, automated enrichment, and multi-dimensional readout, can process hundreds to thousands of samples in parallel in a single experiment. It enables rapid acquisition of binding profiles, differential distribution, and functional correlation information, greatly improving the efficiency and depth of glycan research.
Kinoshita, M. et al. Anal Bioanal Chem. 2021.
Figure 1. High-Throughput N-Glycan Screening
Leveraging advanced analytical platforms and extensive glycobiology expertise, MtoZ Biolabs provides a systematic High-Throughput N-Glycan Screening Service covering everything from initial screening to validation, helping to advance scientific and industrial projects efficiently.
Services at MtoZ Biolabs
MtoZ Biolabs offers a high-throughput N-glycan screening service based on multiple platforms, enabling rapid identification of specific interactions between N-glycan structures and proteins, antibodies, cells, or other molecules. Core screening strategies include:
✔ Glycan Microarray: Immobilizing multiple N-glycan structures on solid supports, detecting binding signals through fluorescently labeled probes, suitable for large-scale specificity profiling.
✔ Bead-Based Screening: Coupling N-glycans to bead surfaces, reacting with target molecules in solution, and detecting binding events via ELISA or flow cytometry.
✔ Fluorescent Probe-Based Screening: Using fluorescently labeled N-glycan probes to analyze binding with target molecules, especially for cell-level studies.
✔ SPR/BLI Label-Free Detection: Real-time monitoring of binding kinetics and affinity parameters between N-glycans and target molecules.
✔ Mass Spectrometry Screening: Using MALDI-TOF or LC-MS/MS platforms for high-throughput structural analysis of bound N-glycans.
Analysis Workflow
1. Project Consultation and Design: Define research objectives, choose the optimal screening strategy, and develop a customized plan.
2. Sample Preparation and Quality Control: Prepare and verify the quality of N-glycan probes or target molecules.
3. High-Throughput Screening Execution: Run the screening process automatically on the selected platform.
4. Data Acquisition and Analysis: Process signals and perform statistical analysis using standardized workflows.
5. Results Delivery and Technical Support: Provide detailed experimental reports, visualized data, and follow-up recommendations.
Service Advantages
1. Multi-Technology Platform Integration
Our High-Throughput N-Glycan Screening Service integrates multiple screening approaches, including microarrays, beads, fluorescent probes, SPR/BLI, and mass spectrometry, which can be flexibly combined based on research objectives, sample characteristics, and throughput requirements, ensuring methodological alignment with project goals.
2. Automated Execution
Our automated screening systems reduce human error and, together with standardized quality control points and data analysis processes, ensure batch-to-batch consistency and traceability, making them suitable for long-term or multi-batch studies.
3. Customized Service
MtoZ Biolabs has a diverse N-glycan structure library and can synthesize special structures on demand to ensure research specificity and innovation.
4. One-Time Charge
Our pricing is transparent, with no hidden fees or additional costs.
Applications
1. Mechanistic studies of N-glycan–receptor protein interactions
2. N-glycan-dependent vaccine antigen design and optimization
3. Screening of cancer- and metabolism-related N-glycan biomarkers
4. Exploration of glycan–glycan interaction mechanisms and glycomimetic drug targets
5. Analysis of antibody or receptor binding specificity to N-glycan structures
FAQs
Q1: Can screening be performed for a specific N-glycan structure?
Yes, we support custom synthesis of specific N-glycan structures or the use of client-provided compounds.
Q2: How long does the High-Throughput N-Glycan Screening Service take?
Routine screening usually takes 2–3 weeks; projects involving custom structures or complex targets may take longer.
Sample Submission Suggestions
1. Protein samples: ≥100 µg, purity >90%, lyophilized or in low-salt buffer
2. Antibodies/Cell lysates: Indicate source, concentration, and storage conditions
3. N-glycan derivatives (client-provided): Provide structure, purity, and solvent information
4. Other samples: Please contact us in advance to confirm compatibility
Deliverables
1. Description of the screening platform and workflow
2. Raw data and processed data tables
3. Visualized results (heatmaps, binding profiles, etc.)
4. Statistical analysis and signal evaluation reports
5. Complete technical report (methods, conditions, and result interpretation)
N-glycans are not only an essential component of cell surface glycans but also a crucial link between structural information and functional regulation. With integrated multi-platform and automated high-throughput capabilities, MtoZ Biolabs provides high-throughput N-glycan screening service to help rapidly identify biologically relevant N-glycan-mediated interactions and accelerate both research and translational applications.
Contact us to obtain a tailored high-throughput N-glycan screening solution for your project.
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High-Throughput Glycan Screening Service
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