Glycomics Service
Glycomics Service aims to systematically characterize the structural complexity, biological functions, and interaction networks of glycans in living systems. Glycans, as essential constituents of the cell membrane and extracellular matrix, play critical roles in cellular recognition, signal transduction, immune regulation, and pathogen interaction. Owing to these multifaceted functions, glycans are often referred to as the "Sugar Code" of life.
The glycome comprises the full spectrum of glycan structures and modifications that vary across physiological and pathological conditions. Its pronounced chemical heterogeneity and spatial-temporal dynamics offer unique insights into complex biological processes. By leveraging multi-platform analytical strategies, glycomics enables in-depth elucidation of glycan composition, glycosidic linkages, branching patterns, and expression profiles.
MtoZ Biolabs offers Glycomics Service through a comprehensive platform that integrates high-resolution mass spectrometry, lectin microarrays, metabolic biotin labeling, and spatial imaging technologies. These services are widely applied across biomedical research, pharmaceutical development, biotechnology, food science, and environmental monitoring, enabling researchers to uncover the functional roles of glycans in health and disease and to identify novel biomarkers and therapeutic targets.
Cummings, R. D. et al. Chem. Biol. 2014.
Figure 1. General Glycomics Strategies
Services at MtoZ Biolabs
1. Glycan Release Service
We provide enzymatic approaches such as PNGase F and O-glycosidase, as well as chemical methods including beta-elimination and acid hydrolysis, to release various glycan types such as N-glycans, O-glycans, and glycolipids from diverse biological samples. These strategies are selected based on sample type and analytical purpose.
2. Glycan Separation and Purification Services
Glycans are efficiently separated and purified using HPLC and solid-phase extraction (SPE) techniques, ensuring high accuracy and reproducibility for downstream structural and quantitative analyses.
3. Glycan Structure Analysis Service
MtoZ Biolabs utilizes MALDI-TOF-MS, LC-MS/MS, GC-MS, and other complementary platforms to achieve high-resolution glycan profiling, isomer differentiation, and three-dimensional conformational elucidation from monosaccharides to intact glycan structures.
Glycan Modification Analysis Service
Glycan Linkage Position Analysis Service
Glycan Linkage Analysis Service
Glycan Linkage Type Analysis Service
Glycan Branching Analysis Service
Glycan Cross-linking Analysis Service
4. Basic Glycan Composition Analysis Service
We provide foundational analyses of glycan composition, classification, and functional group properties to support structural and functional studies.
Monosaccharide Composition Analysis Service
Neutral and Amino Sugar Analysis Service
5. Quantitative Glycomics Service
Comprehensive quantitative analysis covering monosaccharides, oligosaccharides, and total sugars is available through multiple strategies, including label-free quantification, isotopic labeling, and fluorescent tagging, to meet diverse analytical needs.
Single Glycan Quantitative Analysis Service
Total Sugar Quantitative Analysis Service
Why Choose MtoZ Biolabs?
✔ Integrated Multi-Platform Technologies: We combine high-resolution LC–MS/MS, MALDI-TOF, GC-MS, lectin microarrays, metabolic labeling, and spatial imaging to comprehensively resolve the structural complexity of glycans.
✔ Exceptional Structural Resolution: Our Glycomics Service enables precise analysis of glycan linkages, branching topologies, substitution sites, and modification states, supporting multi-level structural elucidation from individual residues to complete glycan chains.
✔ Broad Sample Compatibility: Our platform supports a wide range of biological matrices including serum, tissues, cultured cells, microbial samples, food-based materials, glycoproteins, and polysaccharides.
✔ One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
✔ Customized Service: Tailored to the specific research needs of our clients, we offer flexible experimental design and personalized data analysis to ensure the achievement of research goals to the fullest extent.
Sample Submission Suggestions
MtoZ Biolabs supports glycomics analysis of various sample types, such as serum/plasma, tissues, cultured cells, food samples, glycoproteins, and polysaccharides. Sample volume and preparation requirements may vary depending on the specific service. Please contact us for a detailed sample submission guideline, and our technical specialists will help you determine the most suitable submission strategy for your project.
Applications
Glycomics Service offers powerful analytical support across numerous research areas and industrial applications, including but not limited to:
· Mechanistic Studies of Disease: Investigate glycosylation alterations and regulatory pathways associated with cancer, infection, immune disorders, and neurological diseases.
· Biomarker and Diagnostic Development: Identify disease-specific glycan biomarkers through expression profiling to support early diagnosis, subtype classification, and disease monitoring.
· Vaccine and Therapeutic Design: Reveal pathogen-associated glycan epitopes and host glycosylation targets to inform glycan-based vaccine development and therapeutic innovation.
· Quality Control of Therapeutic Glycoproteins: Characterize glycan heterogeneity in biotherapeutics to ensure batch-to-batch consistency and meet regulatory quality standards.
· Food and Environmental Research: Analyze functional oligosaccharides, microbial glycan metabolites, and sugar contaminants in food products and environmental samples.
FAQ
Q1: How do I choose between N-glycan and O-glycan release strategies?
N-glycans are typically released using specific enzymes such as PNGase F. In contrast, O-glycans often require chemical methods such as beta-elimination due to their high structural heterogeneity. MtoZ Biolabs will evaluate your sample type and research objectives to recommend the most suitable release strategy that ensures data integrity and analytical accuracy.
Q2: Can your glycomics platform distinguish glycan linkage types and positional isomers?
Yes. Our high-resolution mass spectrometry platform, combined with tandem MS, chemical derivatization, and chromatographic separation, enables reliable identification of glycosidic linkage types and positional isomers. This allows for accurate glycan profiling even in highly complex biological systems.
Q3: Do you support absolute quantification of glycans?
Yes. MtoZ Biolabs provides absolute quantification of monosaccharides and glycan structures using internal standards (e.g., isotopically labeled glycans), fluorescence labeling, or external calibration. These methods are ideal for comparative studies, quality control, and quantitative functional analysis.
What Could be Included in the Report?
Upon completion of glycomics analysis, MtoZ Biolabs will provide the following deliverables:
1. Glycan extraction and derivatization protocol records
2. Qualitative and quantitative analysis reports of glycan structures
3. Annotated glycan structure diagrams
4. Technical summary report with functional interpretation insights
5. Raw mass spectrometry data files
Partnering with MtoZ Biolabs for Glycomics Service ensures access to high-resolution structural data and functional insights that can drive both fundamental discoveries and translational outcomes. Contact us today to request a proposal or project quote.
How to order?
