Total Sugar Quantitative Analysis Service

Total sugar refers to the sum of all carbohydrate substances, including monosaccharides, disaccharides, and polysaccharides, which are widely found in various organisms and foods in nature. Using high-resolution technologies combined with derivatization treatments, efficient quantification of total sugars in samples can be achieved. This service provides a comprehensive analysis of the sugars in samples, helping to study the functional changes and metabolic processes of sugars in organisms, ensuring the accuracy and reliability of the data.

 

The total sugar quantitative analysis service has broad applications across multiple fields. In glycomics research, quantifying total sugars helps reveal the role of sugars in cell functions, signal transduction, and disease mechanisms. In the food industry, quantitative analysis of total sugar content effectively controls the quality and safety of food products. In biomedicine, by analyzing the total sugar content in body fluids, the service provides data support for disease monitoring, diagnosis, and prevention. In environmental monitoring, it can be used to detect environmental samples, providing analytical support for environmental protection data.

 



Agius, C. et al. Methods X, 2018.

Figure 1. Analysis of Sugars by HPLC.

 

Services at MtoZ Biolabs

Based on advanced high-resolution instruments, MtoZ Biolabs' total sugar quantitative analysis service can accurately analyze the total content of all carbohydrate components in a sample. Through optimized analytical methods, the service can quantitatively analyze total sugars in different types of samples, covering monosaccharides, disaccharides, and polysaccharides. The analysis results include the total sugar content, the distribution of sugars, and concentration data in various samples. Through precise quantitative analysis, this service helps clients understand the changes in sugars and their functional characteristics in different biological samples, providing strong data support for glycan research, quality control, and disease monitoring. The methods provided by MtoZ Biolabs for quantitative analysis of total sugars include, but are not limited to, the following:

 

1. High-Performance Liquid Chromatography (HPLC)

With optimized chromatographic columns and detection systems, HPLC can precisely measure the total sugar content in samples, making it suitable for the quantitative analysis of sugars in complex samples.

 

2. Mass Spectrometry (MS)

In total sugar quantitative analysis, MS, when coupled with liquid chromatography, enables the rapid and accurate quantification of sugar components, making it ideal for analyzing low-abundance sugars.

 

3. Glycan Microarray Analysis

Glycan microarray analysis can simultaneously detect multiple sugar components and their concentrations, making it suitable for large-scale glycan screening and quantitative analysis.

 

4. Enzymatic Assay

The enzymatic assay method uses specific enzymes to catalyze reactions of sugars, precisely measuring the total sugar content in samples. It is ideal for detecting specific types of sugars and provides quick, reliable quantitative results.

 

Analysis Workflow

1. Sample Preparation

The sample is first pre-treated to remove impurities, ensuring a clean and suitable sample for subsequent analysis.

 

2. Carbohydrate Extraction

Carbohydrates are extracted from the sample using appropriate chemical methods or enzymatic treatment, ensuring complete release of the carbohydrate components for further analysis.

 

3. Derivatization

If necessary, the carbohydrate samples undergo derivatization to enhance their detectability and analysis sensitivity, ensuring more accurate quantification.

 

4. Analysis Method Selection

Based on the sample type and analysis requirements, an appropriate analysis method is selected to separate and quantify the total sugar content in the sample.

 

5. Quantitative Analysis and Data Processing

Standard curves and instrumental data are used to quantify the carbohydrate content in the sample, ensuring the accuracy and reliability of the analysis results.

 

6. Data Interpretation and Report Generation

The analysis results are processed and interpreted, generating a detailed quantitative report that provides the carbohydrate content and related data, supporting subsequent research or quality control.

 

Sample Submission Suggestions

1. Sample Types

This service is suitable for a variety of biological samples containing carbohydrate components, including food products, biological tissues, environmental samples, and fermentation broths. Samples can be either fresh or frozen.

 

2. Sample Purity

Carbohydrates in the sample should be as pure as possible to avoid interference with the analysis. For complex samples, it is recommended to perform preliminary removal of proteins or lipids to ensure accurate results.

 

3. Sample Storage and Transportation

Samples should be transported under low-temperature conditions. Liquid samples can be transported using ice packs or dry ice, while solid samples should be securely sealed and protected from moisture. To ensure sample quality, it is recommended to keep the samples frozen or cold during transportation.

 

Service Advantages

1. High Sensitivity

The service can detect low-abundance carbohydrate components, making it suitable for glycan analysis in complex biological samples, providing sensitive and accurate quantitative data.

 

2. Standardized Process

By utilizing an optimized standardized analytical workflow, the service ensures the efficiency of each analysis step, significantly improving data acquisition efficiency and shortening the analysis cycle.

 

3. Customized Service

Personalized analytical solutions are provided based on the client's specific needs and sample characteristics, offering flexibility to address diverse research challenges and requirements.

 

4. One-Stop Service

From sample preparation, carbohydrate extraction, and analysis to result interpretation, we offer a complete one-stop service, streamlining the experimental process and helping clients efficiently obtain the required data.

 

Applications

1. Glycomics Research

The total sugar quantitative analysis service can be used to analyze the total sugar content in different biological samples, helping to reveal the role of sugars in various biological processes and advancing research in the field of glycomics.

 

2. Biopharmaceutical Quality Control

During the production of biopharmaceuticals such as glycoproteins and glycolipids, total sugar quantification ensures the consistency and stability of product quality, improving the reliability and standardization of the production process.

 

3. Food Industry Applications

The total sugar quantitative analysis service can be used to analyze the total sugar components in food, ensuring the standardization and safety of food formulations, while supporting the development and quality control of functional foods and nutritional products.

 

4. Environmental and Agricultural Research

In plant biology and agricultural research, total sugar quantification of plant samples helps to understand the role of sugars in plant growth, development, and stress resistance, advancing agricultural research and crop improvement.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Data Analysis, Preprocessing, and Estimation

4. Bioinformatics Analysis

5. Raw Data Files