Single Glycan Quantitative Analysis Service

Glycans, as important biomolecules on the cell surface, play a key role in biological processes such as cell recognition and signal transduction. Therefore, quantitative analysis of single glycans is of great significance for studying the biological functions of glycosylation modifications and revealing disease mechanisms. This service provides information on the types, concentrations, and distributions of target glycans in different samples, ensuring high precision and reliability in the analysis results.

 

The single glycan quantitative analysis service is widely applied in various fields. In glycomics research, quantitative analysis of single glycans helps reveal the functions of glycan chains and their changes in both physiological and pathological states. In biopharmaceutical quality control, it ensures the consistency and stability of glycan compositions. In disease biomarker screening, analyzing glycan changes can assist in identifying potential biomarkers, especially for early diagnosis in diseases such as cancer immunology and diabetes.

 

Services at MtoZ Biolabs

Based on high-resolution mass spectrometry platforms, MtoZ Biolabs offers the single glycan quantitative analysis service which provides high-precision quantitative analysis of single glycans. By isolating, enriching, and quantifying the target single glycan, the service accurately identifies the type and concentration of the single glycan and provides detailed quantitative data. The analysis results include the concentration, relative abundance, and distribution of the single glycan in the sample, helping clients gain a deeper understanding of glycan changes and their biological functions.

 

Analysis Workflow

1. Sample Preparation

The sample is first pre-treated to remove impurities, ensuring a clean sample for subsequent analysis.

 

2. Glycan Release and Enrichment

The target single glycan is released from the sample using enzymatic or chemical methods, and specific enrichment techniques are employed to extract the single glycan.

 

3. Separation and Analysis

High-performance liquid chromatography (HPLC) and mass spectrometry (MS) are used to separate, identify, and quantify the released single glycan, accurately identifying its type and concentration.

 

4. Data Processing and Quantification

Standard curves and data processing are employed to provide quantitative analysis results, including the concentration and abundance of the single glycan.

 

5. Result Interpretation and Report

A detailed analysis report is generated, offering both qualitative and quantitative data on the single glycan, with biological significance interpretations.

 

Sample Submission Suggestions

1. Sample Types

This service is suitable for a variety of biological samples containing single glycans, including glycoproteins, glycolipids, cell lysates, tissue extracts, serum, plasma, etc. Samples should be fresh or frozen to avoid glycan loss during handling.

 

2. Sample Transportation

Samples should be transported under low-temperature conditions (such as with ice packs or dry ice) to ensure they are not affected by temperature fluctuations during transit. For frozen samples, dry ice is recommended for transportation.

 

Service Advantages

1. High Precision Quantification

Using advanced liquid chromatography (HPLC) and mass spectrometry (MS) technologies, this service provides highly accurate glycan quantitative analysis, ensuring the precision and reliability of the results.

 

2. High Sensitivity

Capable of detecting low-abundance glycan components, it is suitable for the analysis of complex biological samples, delivering sensitive quantitative results.

 

3. Standardized Process

Optimized standardized analysis workflows and advanced equipment ensure efficient data acquisition, enabling clients to quickly receive analysis results, saving valuable time.

 

4. Customized Service

Personalized analytical solutions are offered based on the client’s research goals and sample characteristics, providing flexibility to address different research needs.

 

Applications

1. Glycomics Research

The single glycan quantitative analysis service can be used to analyze the types and concentrations of single glycans in biological samples, helping to reveal the structural and functional relationships of glycans and advancing the field of glycomics research.

 

2. Biopharmaceutical Quality Control

In the production of glycoproteins, glycosphingolipids, and other biopharmaceuticals, the service monitors the quantitative aspects of glycan components to ensure consistency and stability in product quality.

 

3. Disease Biomarker Screening

By quantitatively analyzing changes in single glycans, this service aids in the identification of potential disease biomarkers, with significant applications in the early diagnosis of diseases such as cancer and immune-related disorders.

 

4. Food and Nutrition Research

The single glycan quantitative analysis service is applicable in food analysis, ensuring the standardization of glycan components in food formulations, supporting the development and quality control of functional foods and nutritional products.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Data Analysis, Preprocessing, and Estimation

4. Bioinformatics Analysis

5. Raw Data Files