Neutral and Amino Sugar Analysis Service
Neutral and amino sugar analysis involves comprehensive profiling of the composition, quantification, and structure of neutral sugars (such as glucose, fructose, galactose) and amino sugars (such as glucosamine, galactosamine) using a range of advanced techniques. Neutral sugars are uncharged monosaccharides found in plant, animal, and microbial cell walls, glycoproteins, and glycolipids. They play a critical role in energy metabolism and serve important biological functions. Amino sugars, which contain amino groups, are typically present in the cell walls of bacteria, fungi, and archaea and possess unique biological properties.
Figure 1. D-Glucosamine Molecular Structure
By precisely analyzing these sugar molecules, researchers can gain valuable insights into their roles in cell recognition, signal transduction, immune responses, and microbial activity. MtoZ Biolabs offers high-accuracy Neutral and Amino Sugar Analysis Service by integrating mass spectrometry, chromatography, NMR, and other analytical platforms. These services support a wide range of applications, including glycomics research, pharmaceutical development, environmental monitoring, and food quality assessment.
Analysis Workflow
MtoZ Biolabs offers Neutral and Amino Sugar Analysis Service powered by advanced analytical technologies to ensure precise profiling and structural insights. Our Neutral and Amino Sugar Analysis Service includes comprehensive sugar composition analysis, accurate quantification of amino sugars, classification of glycosidic bond types, and detection of key modification sites. The workflow is designed to deliver reliable and detailed characterization of complex carbohydrate samples.
1. Sample Preparation and Derivatization
Samples such as blood, urine, plant tissue, or soil are subjected to extraction and purification protocols tailored to each matrix. The PMP (1-phenyl-3-methyl-5-pyrazolone) derivatization method is employed to enhance MS detection sensitivity and ensure data reproducibility.
2. Sugar Separation and Detection
We utilize HPLC, GC, and MS techniques to achieve efficient separation and precise analysis:
HPLC–MS/MS: Enables effective separation and quantification of various neutral and amino sugars.
GC–MS: Ideal for gas-phase analysis, particularly for determining the molecular weights and structural characteristics of derivatized sugars.
3. Amino Sugar Modification Analysis
By combining enzymatic digestion with MS, we analyze glycosidic linkage types, identify connection points between amino sugars and glycan chains, and elucidate structural details of glycosidic bonds.
4. Data Analysis and Reporting
Quantitative and structural data are processed using GlycoWorkbench, Proteome Discoverer, and other specialized software. A detailed technical report is provided, including sugar composition, glycosidic linkage types, and modification site information.
Why Choose MtoZ Biolabs?
✔ Cutting-Edge Instrumentation: Powered by Thermo Fisher Q Exactive HF and Orbitrap Fusion Lumos mass spectrometers, along with Nano-LC systems, our platform ensures high sensitivity and resolution for accurate sugar detection in complex biological matrices.
✔ Comprehensive Qualitative and Quantitative Support: We apply stable isotope labeling and internal standards to deliver precise quantification of both neutral and amino sugars, ensuring reproducibility and data consistency.
✔ Isomer-Specific Analysis Capability: Our approach combines LC, CE, and GC–MS to achieve efficient separation and accurate analysis of sugar isomers, including glycosidic linkage and structural configuration differentiation.
✔ Customized Service Options: We provide flexible, tailored analytical workflows to meet specific research goals, such as quantification needs or detailed glycan structure analysis.
✔ One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Sample Submission Suggestions
Neutral and Amino Sugar Analysis Service is compatible with a wide range of sample types, including glycoproteins, free glycans, tissue extracts, cell lysates, and biological fluids (e.g., serum, plasma, urine). Sample amount and handling requirements vary by project. Please contact our technical team for a complimentary submission guide tailored to your specific application.
Applications
Neutral and Amino Sugar Analysis Service supports diverse applications across multiple fields:
· Biomarker Discovery: Identifying and quantifying sugar distributions in biological samples to support disease biomarker development.
· Pharmaceutical Development and Quality Control: Ensuring consistency in sugar composition, especially for glycosylated drugs.
· Plant and Environmental Glycomics: Studying sugars in plant and soil samples to explore links between microbial activity, environmental health, and agricultural productivity.
· Food Industry and Nutrition Science: Verifying sugar composition in food products and supporting nutritional analysis and quality assurance.
· Microbial and Soil Health Research: Profiling sugar distributions within microbial communities to investigate their relationship with soil health and crop yield.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
FAQ
Q1: Should neutral and amino sugars be analyzed separately? Can they be analyzed together?
Yes, they can be analyzed either separately or concurrently, depending on your project goals. MtoZ Biolabs can optimize derivatization reagents and separation strategies to simultaneously analyze both types of sugars, reducing sample consumption and turnaround time while maintaining high resolution and quantification accuracy.
Q2: Does PMP derivatization affect quantitative accuracy?
PMP derivatization is a widely used and validated method for enhancing LC–MS detection of sugars. At MtoZ Biolabs, we optimize derivatization conditions and apply internal standard strategies to minimize variability, ensuring reliable and reproducible quantification.
Related Services
Glycan Separation and Purification Services
Monosaccharide Composition Analysis Services
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