CE-MS-Based High-Throughput Glycan Screening Solution
Glycans play pivotal roles in essential biological processes, including cell recognition, immune regulation, and signal transduction. With their increasing significance in biological research, drug development, and biopharmaceutical manufacturing, precise and efficient glycan analysis technologies are more critical than ever.
MtoZ Biolabs offers CE-MS-Based High-Throughput Glycan Screening Solution that integrates the unique strengths of capillary electrophoresis (CE) and mass spectrometry (MS) to deliver efficient, accurate glycan profiling and structural analysis. This platform enables rapid screening and precise characterization of diverse glycans, meeting the demands of both research and industrial applications.
Technical Principles
CE-MS merges the high resolution separation of CE with the mass accuracy and structural insight of MS.
● Capillary Electrophoresis (CE) separates charged molecules according to their migration behavior in an electric field, achieving high resolution based on size and charge differences.
● Mass Spectrometry (MS) measures the mass-to-charge ratio (m/z) of ions, providing precise molecular weight and structural information.
The combined approach allows efficient separation of complex glycans with simultaneous acquisition of accurate mass, structural features, and glycosylation modification data.
In glycan analysis, CE-MS excels in high resolution separation, sensitive detection, and rapid profiling, particularly for charged glycans such as N-glycans and O-glycans. Parameters including capillary material, buffer composition, and pH can be optimized for efficient separation and quantification of complex mixtures.
Analysis Workflow
1. Sample Preparation and Glycan Release
Removal of proteins and impurities, enzymatic or chemical release of glycans, followed by optional derivatization for enhanced detection.
2. Capillary Electrophoresis Separation
Injection into the CE system, where glycans are separated under an electric field according to charge and size differences.
3. Mass Spectrometry Detection
Direct introduction of separated glycans into the MS for acquisition of high-precision mass spectra and fragmentation data.
4. Data Acquisition and Quantitative Analysis
Specialized software performs peak identification, quantitative measurement, and preliminary structural characterization.
5. Result Integration and Visualization
Delivery of glycan composition profiles, migration time and accurate mass data, quantitative results, and visual reports.
Shah, U. et al. Int J Pharm Chem Anal. 2021.
Figure 1. CE-MS-Based Glycan Screening Workflow Overview
Sample Submission Suggestions
Our CE-MS-Based High-Throughput Glycan Screening Solution accepts a wide variety of sample types, including glycoproteins, glycolipids, blood, serum, urine, cell lysates, and tissue homogenates. Samples may be submitted in liquid or lyophilized form. High salt, high sugar backgrounds, or strong ion-interfering components should be avoided.
For complex or special matrices, please consult the MtoZ Biolabs technical team in advance. Detailed sample preparation and submission guidelines are available to ensure reliable and reproducible results.
Why Choose MtoZ Biolabs?
✅ Advanced Analysis Platform: Equipped with industry-leading CE-MS systems for high-resolution, rapid glycan separation and high-throughput screening, minimizing sample consumption and analysis time.
✅ Broad Applicability: Suitable for diverse glycan types, including various modification patterns of N-glycans and O-glycans, with applications in proteomics, metabolomics, and drug development.
✅ Multi-Platform Integration: In addition to CE-MS, we offer LC-MS, MALDI-MS, and HILIC for flexible solutions tailored to specific research needs.
✅ Expert Technical Support: Experienced specialists deliver end-to-end services from sample preparation to data interpretation, ensuring high quality and actionable outcomes.
✅ One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Applications
● Drug Development: Screening glycosylation profiles of drug candidates to accelerate design and optimization.
● Biopharmaceutical Quality Control: Monitoring glycan consistency to ensure product quality and stability.
● Metabolomics and Disease Research: Investigating disease related glycosylation changes for biomarker discovery.
● Food and Nutritional Science: Analyzing carbohydrate components in food to evaluate nutritional value and health benefits.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Electropherogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs’ CE-MS-Based High-Throughput Glycan Screening Solution delivers efficient, accurate glycan analysis, enabling faster and more cost-effective acquisition of high quality data. Whether your goal is drug discovery, disease research, or biopharmaceutical quality control, we provide reliable results and customized technical solutions. Contact us to learn how our CE-MS expertise can advance your research and development.
FAQ
Q1: What are the main differences between CE-MS and LC-MS in glycan analysis?
CE-MS is particularly effective for separating charged small molecules and resolving complex glycan modification patterns, while LC-MS offers broader sample compatibility and higher sensitivity for large molecules. MtoZ Biolabs can combine both techniques to create a comprehensive glycan analysis strategy tailored to specific research objectives.
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