HPLC-Based High-Throughput Glycan Screening Solution

High Performance Liquid Chromatography (HPLC), known for its high separation efficiency, resolution, and reproducibility, is a powerful tool for glycan analysis. When combined with high-throughput automated platforms, it enables rapid screening and detailed structural characterization of large sample sets. Leveraging advanced HPLC platforms and specialized glycomics analytical systems, MtoZ Biolabs offers HPLC-Based High-Throughput Glycan Screening Solution that ensures robust, accurate, and reproducible glycan structure analysis for research and industrial needs.

 

Technical Principles

MtoZ Biolabs’ HPLC-Based High-Throughput Glycan Screening Solution employs HPLC as its core analytical technology, achieving high-resolution glycan separation and characterization through interactions between the stationary phase and the mobile phase. This approach effectively resolves structurally diverse glycans with complex modifications and enables both qualitative and quantitative analysis using parameters such as chromatographic peak shape and retention time.

 

To further enhance structural resolution and analytical precision, the solution can be coupled with multiple detection modalities. For instance, integration with Mass Spectrometry (MS) provides direct post-separation determination of molecular weight, modification groups, and ligand information, while coupling with Nuclear Magnetic Resonance (NMR) enables detailed elucidation of glycan chemical structures and conformational features. Depending on specific research objectives, additional detection methods—such as fluorescence detection, ultraviolet detection, or other targeted approaches—can be employed to optimize sensitivity, resolution, and structural insight.

 

In high-throughput configurations, automated sample injection and multi-channel detection systems facilitate continuous processing of large sample batches, ensuring analytical stability and cross-batch data consistency. This foundation supports reliable structural annotation, glycan profile construction, and subsequent statistical analysis.

 

Analysis Workflow

1. Sample Preparation and Glycan Release

Release glycans from samples using enzymatic or chemical methods.

 

2. Glycan Labeling

Derivatize glycans with detectable labels (e.g., fluorescent or chromophoric tags) to enhance detection sensitivity.

 

3. HPLC Separation

Select the appropriate chromatographic mode based on glycan physicochemical properties to achieve optimal separation.

 

4. Detection and Data Acquisition

Apply multiple detection strategies, with optional coupling to MS or NMR for enhanced structural characterization.

 

5. Data Analysis and Glycan Profile Construction

Process and annotate chromatographic data to generate glycan profiles and conduct statistical evaluation.

 

 

Shubhakar, A. et al. Chromatographia. 2015.

Figure 1. Workflow and UHPLC Profile of Automated IgG N-Glycan Analysis

 

Why Choose MtoZ Biolabs?

✅ Highly Stable Separation Platform: Optimized chromatographic conditions ensure data consistency and comparability across batches.

✅ Flexible Multi-Mode Detection: Supports coupling with UV, fluorescence, and mass spectrometry to accommodate diverse research requirements.

✅ High-Throughput Capacity: Automated sample injection and batch data processing significantly reduce analysis turnaround time.

✅ Extensive Expertise in Glycan Analysis: A seasoned technical team with deep experience in chromatographic optimization and complex data interpretation.

✅ Tailored Service Solutions: Customized analytical strategies designed to meet specific project objectives.

 

Sample Submission Suggestions

Our HPLC-Based High-Throughput Glycan Screening Solution is compatible with a wide range of sample types, including glycoproteins, glycolipids, blood, serum, urine, cell lysates, and tissue homogenates. Samples may be submitted in either liquid or lyophilized form. It is recommended to avoid high salt content, pigmented contaminants, or strong UV-absorbing components.

 

For samples with complex matrices or unique properties, please consult the MtoZ Biolabs technical team in advance. We provide comprehensive submission guidelines and customized pre-treatment recommendations to ensure reliable and reproducible results.

 

Applications

● Disease-Associated Glycan Biomarker Research: Investigating glycan structural alterations under various physiological or pathological conditions and their biological implications.

● Biopharmaceutical Quality Control: Monitoring glycan profile distribution to support process optimization and consistency verification.

● Vaccine Glycoantigen Analysis: Facilitating immunogenicity evaluation and research.

● Comparative Host Cell Glycan Profiling: Assessing modification patterns across different cell sources or culture conditions.

● Food and Nutrition Research: Identification and characterization of functional glycan components.

 

What Could be Included in the Report?

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Chromatogram & Quality Control Assessment

4. Data Analysis, Preprocessing, and Estimation

5. Bioinformatics Analysis

6. Raw Data Files

 

MtoZ Biolabs’ HPLC-Based High-Throughput Glycan Screening Solution integrates the stability and precision of high-performance liquid chromatography with the versatility of multi-mode detection. This comprehensive approach provides high-quality, high-throughput glycan analysis for applications in scientific research, drug development, food analysis, and other fields. Backed by a mature technology platform, an expert technical team, and fully customizable service models, we are dedicated to delivering scientifically rigorous, accurate, and application-ready glycan detection and characterization support.

 

Contact MtoZ Biolabs today to obtain your tailored high-throughput glycan screening solution.

 

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