ESI-MSn-Based High-Throughput Glycan Screening Solution
ESI-MSn-Based High-Throughput Glycan Screening Solution combines the high ionization efficiency of electrospray ionization (ESI) with the structural elucidation capabilities of multi-stage mass spectrometry (MSⁿ), providing a rapid, accurate, and reproducible approach for analyzing glycans. Within a single experiment, it delivers detailed information on glycan composition, linkage patterns, branching structures, and modifications, making it well suited for high-throughput screening and comprehensive characterization of complex glycan systems.
Leveraging high-resolution mass spectrometry and automated sample handling, MtoZ Biolabs offers complete end-to-end support, from rapid analysis of large sample sets to result visualization and structural interpretation. Our ESI-MSn-Based High-Throughput Glycan Screening Solution is widely applicable in glycomics research, metabolomics studies, glycan-based drug development, and modification site verification, delivering high sensitivity and deep structural insights to advance your projects.
Technical Principles
ESI gently converts molecules in solution into charged ions by applying a high-voltage electric field. The sample passes through a spray needle or capillary to form fine droplets, and as the solvent evaporates, molecules become ionized and enter the gas phase, preserving native glycan structures to the greatest extent.
MSⁿ performs consecutive tandem mass spectrometry scans, sequentially selecting and fragmenting precursor ions to generate a complete series of fragment ions. This enables not only molecular weight determination but also detailed analysis of glycan composition, linkage patterns, branching architectures, ring structures, stereochemistry, and precise localization of modifications such as acetylation, methylation, and phosphorylation.
By integrating ESI with MSⁿ, the platform achieves both highly sensitive quantification and comprehensive structural elucidation, making it ideal for systematic, high-throughput characterization of complex glycans.
Gu, Y. et al. Front. Anal. Sci. 2022.
Figure 1. ESI-MS Principle
Analysis Workflow
1. Experimental Design and Parameter Optimization
Tailor ionization voltage, flow rate, collision energy, and multi-stage scanning parameters to the glycan type, modification profile, and research objectives, ensuring optimal signal intensity and structural detail.
2. Sample Preparation
Remove interfering components through purification, concentration, or derivatization when necessary to enhance ionization efficiency and detection sensitivity.
3. ESI Ionization
Nebulize the sample under high voltage to produce charged droplets, which generate gas-phase ions during desolvation and are transferred into the mass spectrometer.
4. MSⁿ Analysis
Sequentially isolate and fragment precursor ions using techniques such as collision-induced dissociation (CID) or higher-energy collisional dissociation (HCD) to obtain complete and informative structural fragment spectra.
5. Data Processing and Reporting
Apply glycan-specific databases and advanced algorithms to match and annotate fragment ions, define glycan composition, linkage arrangements, and modification types and positions, and compile a detailed, structured analytical report.
Why Choose MtoZ Biolabs?
✅ Advanced Analysis Platform: Equipped with high-resolution ESI-MSn instrumentation and proven multi-stage data acquisition workflows to deliver deep structural insights into complex glycans.
✅ High Throughput and Sensitivity: Capable of rapid screening and quantitative analysis of large sample sets, with reliable detection of low-abundance glycans.
✅ Precise Modification Mapping: Concurrent identification and localization of acetylation, methylation, phosphorylation, and other modifications, providing critical information for functional glycomics studies.
✅ Broad Analytical Scope: Applicable to N-glycans, O-glycans, neutral and acidic glycans, and various modifications, supporting research in biopharmaceuticals, disease mechanisms, and metabolomics.
✅ One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Sample Submission Suggestions
Our ESI-MSn-Based High-Throughput Glycan Screening Solution accommodates a wide range of sample types, including glycoproteins, glycolipids, blood, serum, urine, cell lysates, and tissue homogenates, and is compatible with both purified preparations and complex biological matrices. Avoid high salt concentrations and substances that severely inhibit ionization.
For special or challenging samples, contact the MtoZ Biolabs technical team in advance. We will provide comprehensive submission guidelines and customized pre-treatment protocols to ensure analytical accuracy and reproducibility.
What Could be Included in the Report?
1. Comprehensive experimental details
2. Materials, instruments, and methods
3. MSⁿ spectra and quality control assessments
4. Data analysis, preprocessing, and quantitative estimation
5. Bioinformatics analysis
6. Raw data files
With an established multi-stage mass spectrometry platform and extensive expertise in glycomics, MtoZ Biolabs’ ESI-MSn-Based High-Throughput Glycan Screening Solution enables efficient and accurate glycan structural elucidation, modification detection, isomer differentiation, and low-abundance component analysis. By integrating advanced separation techniques and bioinformatics tools, we deliver complete workflow support—from data acquisition to structural interpretation—empowering impactful outcomes in disease research, drug development, and functional glycan discovery.
Contact MtoZ Biolabs today to discuss your research goals with our expert team, and let our solutions drive technical excellence and innovation in your projects.
FAQ
Q1: How effective is this method for resolving glycan isomers?
ESI-MSn offers exceptional isomer differentiation capabilities. Its multi-stage fragmentation spectra generate characteristic fragment ions that distinguish glycans with identical molecular weights but different structures, including branch position isomers, stereoisomers, and linkage isomers. Optimized collision energy gradients further enhance the sensitivity and precision of isomer discrimination.
Q2: Can it be combined with other separation techniques?
Yes. ESI-MSn can be seamlessly integrated with separation techniques such as HPLC, CE, and UHPLC to achieve efficient glycan separation and precise structural elucidation. This multi-dimensional analytical approach increases characterization depth for complex samples and provides a comprehensive data foundation for high-throughput glycomics research.
Related Services
High-Throughput Glycan Screening Solution
Automated LC-MS-Based High-Throughput Glycan Screening Solution
CE-MS-Based High-Throughput Glycan Screening Solution
MALDI-TOF-MS-Based High-Throughput Glycan Screening Solution
How to order?
